Mar. Drugs 2013, 11(4), 1235-1255; doi:10.3390/md11041235
Article

Structural and Immunochemical Studies of the Lipopolysaccharide from the Fish Pathogen, Aeromonas bestiarum Strain K296, Serotype O18

1 Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Akademicka 19, Lublin 20-033, Poland 2 Division of Immunochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Parkallee 10, D-23845 Borstel, Germany 3 Department of Fish Diseases, National Veterinary Research Institute, Partyzantow 57, Pulawy 24-100, Poland 4 Division of Structural Biochemistry, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Airway Research Center North (ARCN), Member of the German Center for Lung Research (DZL), Parkallee 4a/c, D-23845 Borstel, Germany
* Author to whom correspondence should be addressed.
Received: 25 February 2013; in revised form: 8 March 2013 / Accepted: 18 March 2013 / Published: 17 April 2013
(This article belongs to the Special Issue Marine Lipopolysaccharides)
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Abstract: Chemical analyses and mass spectrometry were used to study the structure of the lipopolysaccharide (LPS) isolated from Aeromonas bestiarum strain K296, serotype O18. ESI-MS revealed that the most abundant A. bestiarum LPS glycoforms have a hexa-acylated or tetra-acylated lipid A with conserved architecture of the backbone, consisting of a 1,4′-bisphosphorylated β-(1→6)-linked d-GlcN disaccharide with an AraN residue as a non-stoichiometric substituent and a core oligosaccharide composed of Kdo1Hep6Hex1HexN1P1. 1D and 2D NMR spectroscopy revealed that the O-specific polysaccharide (OPS) of A. bestiarum K296 consists of a branched tetrasaccharide repeating unit containing two 6-deoxy-l-talose (6dTalp), one Manp and one GalpNAc residues; thus, it is similar to that of the OPS of A. hydrophila AH-3 (serotype O34) in both the sugar composition and the glycosylation pattern. Moreover, 3-substituted 6dTalp was 2-O-acetylated and additional O-acetyl groups were identified at O-2 and O-4 (or O-3) positions of the terminal 6dTalp. Western blots with polyclonal rabbit sera showed that serotypes O18 and O34 share some epitopes in the LPS. The very weak reaction of the anti-O34 serum with the O-deacylated LPS of A. bestiarum K296 might have been due to the different O-acetylation pattern of the terminal 6dTalp. The latter suggestion was further confirmed by NMR.
Keywords: lipopolysaccharide; LPS; O-specific polysaccharide; OPS; Aeromonas bestiarum; fish pathogen; 6dTal

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MDPI and ACS Style

Turska-Szewczuk, A.; Lindner, B.; Komaniecka, I.; Kozinska, A.; Pekala, A.; Choma, A.; Holst, O. Structural and Immunochemical Studies of the Lipopolysaccharide from the Fish Pathogen, Aeromonas bestiarum Strain K296, Serotype O18. Mar. Drugs 2013, 11, 1235-1255.

AMA Style

Turska-Szewczuk A, Lindner B, Komaniecka I, Kozinska A, Pekala A, Choma A, Holst O. Structural and Immunochemical Studies of the Lipopolysaccharide from the Fish Pathogen, Aeromonas bestiarum Strain K296, Serotype O18. Marine Drugs. 2013; 11(4):1235-1255.

Chicago/Turabian Style

Turska-Szewczuk, Anna; Lindner, Buko; Komaniecka, Iwona; Kozinska, Alicja; Pekala, Agnieszka; Choma, Adam; Holst, Otto. 2013. "Structural and Immunochemical Studies of the Lipopolysaccharide from the Fish Pathogen, Aeromonas bestiarum Strain K296, Serotype O18." Mar. Drugs 11, no. 4: 1235-1255.

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