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Mar. Drugs 2013, 11(4), 1256-1270; doi:10.3390/md11041256
Article

Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae

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Received: 15 January 2013; in revised form: 6 March 2013 / Accepted: 7 March 2013 / Published: 17 April 2013
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Abstract: A growing market for novel antioxidants obtained from non-expensive sources justifies educated screening of microalgae for their potential antioxidant features. Characterization of the antioxidant profile of 18 species of cyanobacteria (prokaryotic microalgae) and 23 species of (eukaryotic) microalgae is accordingly reported in this paper. The total antioxidant capacity, accounted for by both water- and lipid-soluble antioxidants, was evaluated by the (radical cation) ABTS method. For complementary characterization of cell extracts, a deoxyribose assay was carried out, as well as a bacteriophage P22/Salmonella-mediated approach. The microalga Scenedesmus obliquus strain M2-1 exhibited the highest (p > 0.05) total antioxidant capacity (149 ± 47 AAU) of intracellular extracts. Its scavenger activity correlated well with its protective effects against DNA oxidative damage induced by copper(II)-ascorbic acid; and against decay in bacteriophage infection capacity induced by H2O2. Finally, performance of an Ames test revealed no mutagenic effects of the said extract.
Keywords: 16S rDNA; ABTS•+; deoxyribose; DNA; phage P22/Salmonella; Ames test 16S rDNA; ABTS•+; deoxyribose; DNA; phage P22/Salmonella; Ames test
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Guedes, A.C.; Gião, M.S.; Seabra, R.; Ferreira, A.C.S.; Tamagnini, P.; Moradas-Ferreira, P.; Malcata, F.X. Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae. Mar. Drugs 2013, 11, 1256-1270.

AMA Style

Guedes AC, Gião MS, Seabra R, Ferreira ACS, Tamagnini P, Moradas-Ferreira P, Malcata FX. Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae. Marine Drugs. 2013; 11(4):1256-1270.

Chicago/Turabian Style

Guedes, A. C.; Gião, Maria S.; Seabra, Rui; Ferreira, A. C.S.; Tamagnini, Paula; Moradas-Ferreira, Pedro; Malcata, F. X. 2013. "Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae." Mar. Drugs 11, no. 4: 1256-1270.



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