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Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae
CBQF/Biotechnology College, Catholic University of Portugal, Rua Dr. António Bernardino de Almeida, Porto P-4200-072, Portugal
CIMAR/CIIMAR—Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas nº 177, Porto P-4050-123, Portugal
IBMC—Institute for Molecular and Cell Biology, University of Porto, Rua do Campo Alegre nº 823, Porto P-4150-180, Portugal
Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, Edifício FC4, Porto P-4169-007, Portugal
ICBAS—Institute of Biomedical Sciences Abel Salazar, University of Porto, Largo Abel Salazar nº 2, Porto P-4099-003, Portugal
Department of Chemical Engineering, University of Porto, Rua Dr. Roberto Frias, Porto P-4200-465, Portugal
* Author to whom correspondence should be addressed.
Received: 15 January 2013; in revised form: 6 March 2013 / Accepted: 7 March 2013 / Published: 17 April 2013
Abstract: A growing market for novel antioxidants obtained from non-expensive sources justifies educated screening of microalgae for their potential antioxidant features. Characterization of the antioxidant profile of 18 species of cyanobacteria (prokaryotic microalgae) and 23 species of (eukaryotic) microalgae is accordingly reported in this paper. The total antioxidant capacity, accounted for by both water- and lipid-soluble antioxidants, was evaluated by the (radical cation) ABTS method. For complementary characterization of cell extracts, a deoxyribose assay was carried out, as well as a bacteriophage P22/Salmonella-mediated approach. The microalga Scenedesmus obliquus strain M2-1 exhibited the highest (p > 0.05) total antioxidant capacity (149 ± 47 AAU) of intracellular extracts. Its scavenger activity correlated well with its protective effects against DNA oxidative damage induced by copper(II)-ascorbic acid; and against decay in bacteriophage infection capacity induced by H2O2. Finally, performance of an Ames test revealed no mutagenic effects of the said extract.
Keywords: 16S rDNA; ABTS•+; deoxyribose; DNA; phage P22/Salmonella; Ames test
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MDPI and ACS Style
Guedes, A.C.; Gião, M.S.; Seabra, R.; Ferreira, A.C.S.; Tamagnini, P.; Moradas-Ferreira, P.; Malcata, F.X. Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae. Mar. Drugs 2013, 11, 1256-1270.
Guedes AC, Gião MS, Seabra R, Ferreira ACS, Tamagnini P, Moradas-Ferreira P, Malcata FX. Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae. Marine Drugs. 2013; 11(4):1256-1270.
Guedes, A. C.; Gião, Maria S.; Seabra, Rui; Ferreira, A. C.S.; Tamagnini, Paula; Moradas-Ferreira, Pedro; Malcata, F. X. 2013. "Evaluation of the Antioxidant Activity of Cell Extracts from Microalgae." Mar. Drugs 11, no. 4: 1256-1270.