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Mar. Drugs 2013, 11(4), 1140-1151; doi:10.3390/md11041140
Article

Spongiatriol Inhibits Nuclear Factor Kappa B Activation and Induces Apoptosis in Pancreatic Cancer Cells

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Received: 26 December 2012; in revised form: 14 February 2013 / Accepted: 19 March 2013 / Published: 2 April 2013
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Abstract: Pancreatic cancer, the fourth leading cause of cancer death in the US, is highly resistant to all current chemotherapies, and its growth is facilitated by chronic inflammation. The majority of pro-inflammatory cytokines initiate signaling cascades that converge at the activation of the Nuclear Factor Kappa B (NFκB), a signal transduction molecule that promotes cell survival, proliferation and angiogenesis. In an effort to identify novel inhibitors of NFκB, the HBOI library of pure compounds was screened using a reporter cell line that produces luciferin under the transcriptional control of NFκB. Seven compounds were identified through this screen, but in the case of five of them, their reported mechanism of action made them unlikely to be specific NFκB inhibitors. Spongiatriol, a marine furanoditerpenoid that was first isolated in the 1970s, is shown here to inhibit NFκB transcriptional activity in a reporter cell line, to reduce levels of phosphorylated (active) NFκB in the AsPC-1 cell line, to have an IC50 for cytotoxicity in the low micromolar range against the AsPC-1, BxPC-3, MiaPaCa-2 and Panc-1 pancreatic cancer cell lines, and to induce moderate but significant apoptosis in both the AsPC-1 and the Panc-1 cell lines.
Keywords: inflammation; pancreatic cancer; nuclear factor kappa B; apoptosis inflammation; pancreatic cancer; nuclear factor kappa B; apoptosis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Guzmán, E.; Maher, M.; Temkin, A.; Pitts, T.; Wright, A. Spongiatriol Inhibits Nuclear Factor Kappa B Activation and Induces Apoptosis in Pancreatic Cancer Cells. Mar. Drugs 2013, 11, 1140-1151.

AMA Style

Guzmán E, Maher M, Temkin A, Pitts T, Wright A. Spongiatriol Inhibits Nuclear Factor Kappa B Activation and Induces Apoptosis in Pancreatic Cancer Cells. Marine Drugs. 2013; 11(4):1140-1151.

Chicago/Turabian Style

Guzmán, Esther; Maher, Michael; Temkin, Alexis; Pitts, Tara; Wright, Amy. 2013. "Spongiatriol Inhibits Nuclear Factor Kappa B Activation and Induces Apoptosis in Pancreatic Cancer Cells." Mar. Drugs 11, no. 4: 1140-1151.


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