Next Article in Journal
6″-Debromohamacanthin A, a Bis (Indole) Alkaloid, Inhibits Angiogenesis by Targeting the VEGFR2-Mediated PI3K/AKT/mTOR Signaling Pathways
Previous Article in Journal
Bioactive Compounds from the Red Sea Marine Sponge Hyrtios Species
Previous Article in Special Issue
Iodinin (1,6-Dihydroxyphenazine 5,10-Dioxide) from Streptosporangium sp. Induces Apoptosis Selectively in Myeloid Leukemia Cell Lines and Patient Cells
Mar. Drugs 2013, 11(4), 1071-1086; doi:10.3390/md11041071
Article

Sponge-Derived Kocuria and Micrococcus spp. as Sources of the New Thiazolyl Peptide Antibiotic Kocurin

1
, 1
, 1
, 1
, 1
, 2,†
, 2
, 1
, 1
 and 1,*
Received: 19 December 2012; in revised form: 22 January 2013 / Accepted: 19 February 2013 / Published: 28 March 2013
(This article belongs to the Special Issue Marine Antibiotics)
View Full-Text   |   Download PDF [360 KB, uploaded 28 March 2013]   |   Browse Figures
Abstract: Forty four marine actinomycetes of the family Microccocaceae isolated from sponges collected primarily in Florida Keys (USA) were selected from our strain collection to be studied as new sources for the production of bioactive natural products. A 16S rRNA gene based phylogenetic analysis showed that the strains are members of the genera Kocuria and Micrococcus. To assess their biosynthetic potential, the strains were PCR screened for the presence of secondary metabolite genes encoding nonribosomal synthetase (NRPS) and polyketide synthases (PKS). A small extract collection of 528 crude extracts generated from nutritional microfermentation arrays was tested for the production of bioactive secondary metabolites against clinically relevant strains (Bacillus subtilis, methicillin-resistant Staphylococcus aureus (MRSA), Acinetobacter baumannii and Candida albicans). Three independent isolates were shown to produce a new anti-MRSA bioactive compound that was identified as kocurin, a new member of the thiazolyl peptide family of antibiotics emphasizing the role of this family as a prolific resource for novel drugs.
Keywords: marine actinomycete; sponge; Kocuria; Microccocus; antibiotic; MRSA; thiazolyl peptide; NRPS; PKS marine actinomycete; sponge; Kocuria; Microccocus; antibiotic; MRSA; thiazolyl peptide; NRPS; PKS
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Palomo, S.; González, I.; de la Cruz, M.; Martín, J.; Tormo, J.R.; Anderson, M.; Hill, R.T.; Vicente, F.; Reyes, F.; Genilloud, O. Sponge-Derived Kocuria and Micrococcus spp. as Sources of the New Thiazolyl Peptide Antibiotic Kocurin. Mar. Drugs 2013, 11, 1071-1086.

AMA Style

Palomo S, González I, de la Cruz M, Martín J, Tormo JR, Anderson M, Hill RT, Vicente F, Reyes F, Genilloud O. Sponge-Derived Kocuria and Micrococcus spp. as Sources of the New Thiazolyl Peptide Antibiotic Kocurin. Marine Drugs. 2013; 11(4):1071-1086.

Chicago/Turabian Style

Palomo, Sara; González, Ignacio; de la Cruz, Mercedes; Martín, Jesús; Tormo, José R.; Anderson, Matthew; Hill, Russell T.; Vicente, Francisca; Reyes, Fernando; Genilloud, Olga. 2013. "Sponge-Derived Kocuria and Micrococcus spp. as Sources of the New Thiazolyl Peptide Antibiotic Kocurin." Mar. Drugs 11, no. 4: 1071-1086.


Mar. Drugs EISSN 1660-3397 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert