- freely available
Mar. Drugs 2013, 11(3), 623-642; doi:10.3390/md11030623
Published: 1 March 2013
Abstract: Conotoxins are small peptides present in the venom of cone snails. The snail uses this venom to paralyze and capture prey. The constituent conopeptides display a high level of chemical diversity and are of particular interest for scientists as tools employed in neurological studies and for drug development, because they target with exquisite specificity membrane receptors, transporters, and various ion channels in the nervous system. However, these peptides are known to contain a high frequency and variability of post-translational modifications—including sometimes O-glycosylation—which are of importance for biological activity. The potential application of specific conotoxins as neuropharmalogical agents and chemical probes requires a full characterization of the relevant peptides, including the structure of the carbohydrate part. In this review, the currently existing knowledge of O-glycosylation of conotoxins is described.
The marine predatory cone snails (genus Conus, family Conidae) comprise a large group of circa 800 different species, which are found predominantly in the West Atlantic, Caribbean, and Indo-Pacific tropical season or near coral reefs. They are a remarkable species, not only for the beauty of their conical, colorful shells (Figure 1 and [1,2]) but, in particular, for the neurotoxic compounds they use in the killing of their prey and defending themselves from predators. These compounds, called conotoxins or conopeptides, are synthesized in the venom gland of the snails [3,4]. It has been estimated that approximately 100,000 different peptides can potentially be expressed in the venoms of the entire current Conus genus [5,6,7,8]. However, recent studies have shown that venoms from individual cone snails revealed an unprecedented level of conopeptide diversity, expanding the predicted numbers to well in excess of 1000 unique peptides per Conus species [9,10,11,12]. It has to be noted that cone snail venoms also contain high molecular mass (glycosylated) proteins that participate in the envenomation cocktail [13,14], but the discussion of these compounds is out of the scope of this review.
When attacking their prey, most of the fish-hunting cone snails (e.g., C. striatus, C. magus, C. stercusmuscarum and C. consors) inject deadly venom by means of a disposable harpoon-like radular tooth into the fish (~3–50 μL at a velocity of ~200 m/s) and immobilize the fish within 2 to 3 s, before engulfing it with a large distensible rostrum . In contrast, C. geographus uses a “net strategy” to capture the prey and to sting the fish after engulfing it first. Occasionally, humans have been injured or even killed by the sting from a cone snail, mostly C. geographus [16,17,18].
For many years, it has been claimed that Conus peptides could have great promising therapeutic applications as novel pharmacological substances in neuroscience, because they can specifically target with high affinity and specificity neurotransmitter-gated receptors and voltage-gated or ligand-gated ion channels [19,20,21,22,23,24,25].Typical examples for treatment are neurological disorders such as ischemia, spinal cord injury, Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, chronic pain, stroke, epilepsy, and schizophrenia. Currently, several conotoxins are undergoing human clinical trials as therapeutic drugs [26,27,28,29,30]. One of them, ω-conotoxin MVIIA from C. magus venom (an N-type Ca2+ channel antagonist) is, as a synthetic product, already on the market (SNX-111/Ziconotide/Prialt™) for intrathecal treatment of severe chronic pain [31,32,33]; it is 10,000-fold more powerful than morphine. More than a billion people worldwide suffer from neuropathic pain syndromes, such as chronic pain frequently resulting from cancer, AIDS, arthritis, or injuries [34,35].
Most of the neurobiological active conopeptides are composed of 10 to 35 amino acid residues (molecular mass <5 kDa). The conopeptides contain multiple disulfide bonds and are decorated with a high variety of post-translational modifications (which can occur for up to 75% of the amino acids of a single conotoxin), leading to an exceptional diversity in peptide structures. The types of post-translational modifications found so far comprise: disulfide-bridge formation; hydroxylation of proline at C-4, lysine at C-5 and valine at γ-position; γ-carboxylation of glutamic acid (vitamin K-dependent); bromination of tryptophan at C-6; phosphorylation and sulfation of tyrosine; epimerization of L- to D-amino acids, including tryptophan, leucine, phenylalanine and valine; C-terminal amidation; N-terminal pyroglutamylation (cyclization); and O-glycosylation of serine or threonine.
Several comprehensive reviews have been published on the biology/biochemistry of conotoxins [36,37,38,39,40,41,42,43,44,45,46]. The review by Buczek et al. (2005)  included O-glycosylation data available up until 2005. Here, we will review in detail the O-glycosylation data of conopeptides as known in 2012.
2. The ConoServer Database
A specialized database for conotoxins, called ConoServer, is available online [8,47,48]. The ConoServer provides up-to-date information on the sixteen known conopeptide gene superfamilies and currently contains data for over 3500 conopeptide sequences. The ConoMass tool matches peptide masses predicted from transcripts with a list of masses obtained experimentally by proteomics analysis of cone snail venoms. Several post-translational modifications can be selected. However, it should be noted that glycosylation is not included in ConoMass because of the enormous number of possible structures glycosylation can present. Although the O-glycosylated conopeptides discussed in this review are included in the ConoServer, glycan information is available only for contulakin-G.
3. General Structural Data of Mucin-Type Glycoconjugate O-Glycans
O-Glycosylation is a common type of post(co)-translational modification of proteins in nature, resulting in the attachment of carbohydrates to hydroxyl groups of certain hydroxyl amino acid residues in the peptide backbone. Much is known about the genes and enzymes responsible for the biosynthesis of these kinds of glycoconjugates in mammalian cells . The types of glycans discussed in this review belong to the so-called mucin-type glycoconjugate O-glycans. They are characterized by the occurrence of a carbohydrate-amino acid bond between N-acetyl-α-D-galactosamine and the hydroxyl function of L-serine or L-threonine. In general, the carbohydrate chains are built up from the N-acetyl-hexosamines (HexNAc) N-acetyl-D-galactosamine (GalNAc) and N-acetyl-D-glucosamine (GlcNAc), the hexose (Hex) D-galactose (Gal), the 6-deoxyhexose L-fucose (Fuc), and members of the sialic acid family (mainly N-acetyl-neuraminic acid and N-glycolyl-neuraminic acid). In addition, inorganic sulfate has been found as a substituent of N-acetyl-D-glucosamine and D-galactose. In the mucin-type O-glycans, three structural domains can be distinguished: the core structure, the backbone structure, and the peripheral structure. So far, besides α-D-GalpNAc-(1→O) (core-type 0), nine different core types have been described (Table 1) [49,50,51,52].
|Table 1. Known core structures of mucin-type O-linked glycans.|
4. Glycosylated Conotoxins
4.1. Conus striatus
C. striatus is a fish-hunting Indo-Pacific cone snail. The mature neurotoxic conopeptide κA-conotoxin SIVA (κA-SIVA, also called s4a) isolated from its venom has been shown to be active on tetrodotoxin-sensitive voltage-gated sodium (Nav) channels —though not on voltage-gated potassium channels as thought earlier —thus eliciting spastic paralytic symptoms when injected into the fish during prey capture. The conotoxin induces intense repetitive firing of the frog neuromuscular junction leading to a tetanic contracture in muscle fiber . It has a backbone of 30 amino acids with pyroglutamic acid at the N-terminal site, three 4-trans-hydroxyprolines, amidated cysteine at the C-terminal side, and three disulfide bonds. The peptide contains three serine residues, with a glycan at one of them, and three threonine residues. The primary structure of the glycopeptide is included in Table 2.
Using electrospray-mass spectrometry (ESI-MS), a Hex3HexNAc2 glycan moiety (892.8 Da) was identified at Ser-7 . This was the first evidence for O-glycosylation as a post-translational modification in a biological active conopeptide. The MS/MS spectrum of the peptide revealed HexNAc2, HexHexNAc2, Hex2HexNAc2 and Hex3HexNAc2 fragment ions, and showed losses of one, two and three Hex residues from the intact pseudomolecular ion. The nature of the monosaccharides and type of linkages were not determined. Later, the Hex3HexNAc2 glycan moiety (893 Da) of κA-SIVA (s4a) was confirmed by Jakubowski et al.  using LC/ESI-MS, and by Kelley et al.  using MALDI-TOF-MS and LC/ESI-MS. Evidence was presented for the occurrence of a HexNAc-HexNAc fragment, to which three Hex residues are connected. MS analysis of the ammonia-treated material (β-elimination reaction removing the O-glycan) supported the MS results of the native material, but there was no information about the nature and linkage types of the sugar units.
|Table 2. Overview of O-glycosylated conotoxins.|
|Conus species||Diet||Conotoxin name(s)||Glycopeptide sequence||O-linkedresidue||O-glycan||References|
|C. stercusmuscarum||P||SmIVA||ZTWLVPS*T*ITTCCGYDOGTMCOTCMCDNTCKOKOKKS-NH2||Ser-7||No information|||
|C. stercusmuscarum||P||SmIVB||AOWLVPS*T*ITTCCGYDOGSMCOOCMCNNTCKOKOKKS-NH2||Ser-7||No information|||
|C. magus||P||κA-MIVA||AOγLVVT*AT*TNCCGYNOMTICOOCMCTYSCOOKRKO-NH2||Thr-7||Hex4HexNAc2 as sum of both sites|||
|C. geographus||P||contulakin-G||ZSEEGGSNAT*KKPYIL||Thr-10||β-D-Galp-(1→3)-α-D-Galp NAc||[36,59,60,61,62,63]|
|(tx5a, TxVa or Tx-012)|
P = piscivorous; M = molluscivorous; Z = pyroglutamic acid; S* = glycosylated serine; T* = glycosylated threonine; O = 4-trans-hydroxyproline; γ = γ-carboxyglutamic acid; W* = 6-bromotryptophan.
Preliminary results with the synthetic non-glycosylated κA-conotoxin analog indicated that this was far less potent when injected into animals than the native glycosylated κA-conotoxin . Suggested plausible roles for the O-glycosylation included increasing the on-time and/or affinity of the peptide for its ion channel and increasing the speed of access of the peptide to the channels. See also a mini-review by Craig et al. .
The homologous conotoxin, κA-conotoxin SIVB (κA-SIVB, also termed s4b) is built up from 37 amino acids with N-terminal pyroglutamic acid, C-terminal amidated 4-hydroxyproline, and three disulfide bridges . The primary structure of the conopeptide is included in Table 2.
κA-SIVB, in combination with κA-SIVA, is a major component of the injected venom of C. striatus  and has a similar neuroexcitatory profile as κA-SIVA. In fact, both conotoxins mimic the biological effects of the completely injected venom on fish prey. κA-SIVB was also reported to be glycosylated at Ser-7 [53,56,57]. Based on MALDI-TOF-MS and LC/ESI-MS combined with ammonia treatment, the occurrence of Hex3HexNAc2 was suggested , a similar type of glycosylation as found for κA-SIVA (s4a).
4.2. Conus geographus
The major peptide in the venom of the fish-hunting C. geographus, which lives in the Philippine seas, is a 16-amino acid glycopeptide called contulakin-G . This conotoxin, a neurotensin subtype 1 (NTS1) receptor agonist, targets G-protein-coupled receptors. It was shown to be a potent analgesic when administered intrathecally in animal models [67,68]. Peptide analysis studies showed the presence of a modified Thr-10 residue. A further post-translational modification is a pyroglutamic acid residue at the N-terminal site. The primary structure of the glycopeptide is included in Table 2.
A combination of MALDI-TOF-MS, LSI-MS, and ESI-MS determined that the major O-glycoform corresponded with the Hex-HexNAc sequence. Additionally, three less abundant glycosylated forms were observed, i.e., SO4(HexHexNAc), Hex3, and Hex2HexNAc2 . Enzymatic experiments (a β-D-galactosidase preferentially hydrolyzing (β1→3)-linked D-galactopyranosyl residues and an O-glycosidase treatment liberating a disaccharide) and MALDI-TOF-MS identified the major O-glycoform as a core-1 type structure, β-D-Galp-(1→3)-α-D-GalpNAc-(1→O)-, as depicted in Scheme 1. This is the T-antigen, one of the most common eukaryotic O-glycan structures . The native contulakin-G coeluted on RP-HPLC with synthetic contulakin-G containing the same disaccharide .
When administered to mice, the synthetic glycopeptide produced similar neurological effects as found for the native material (motor control-associated dysfunction). However, the glycosylated form was active at 10-fold lower doses than the non-glycosylated form. In contrast, comparing the binding activities of the synthetic glycopeptide and the synthetic peptide for a number of neurotensin receptor types yielded weaker affinities for the glycosylated material. The different results between the in vivo and in vitro studies, when focused on the importance of the O-glycosylation, are contradictory. Additional studies showed that the proteolytic degradation of contulakin-G is inhibited by the presence of the O-glycan, which may lead to an enhanced supply in vivo of the glycopeptides to the receptor. However, alternative explanations were not excluded and should be studied in more detail [36,42,59]. The glycosylated form is, in fact, a very potent broad-spectrum analgesic, being two orders of magnitude more potent than the non-glycosylated form in vivo [19,69]. It should be noted that contulakin-G has entered phase II clinical trials for short-term management of post-operative pain.
In a few related studies, NMR spectroscopy was used to investigate the three-dimensional structure of contulakin-G and some synthetic analogs, although the analogs showed lower bioactivity than the native contulakin-G.
In the first study, NMR solution conformations were reported for native contulakin-G with β-D-Galp-(1→3)-α-D-GalpNAc-(1→O)- at Thr-10, its non-glycosylated variant, and two glycopeptide analogs, one containing α-D-GalpNAc-(1→O)- at Thr-10 and the other containing β-D-Galp-(1→3)-α-D-GalpNAc-(1→O)- at Ser-7 . It was found that all four substances have mainly random coil peptide conformations. Interestingly, in the glycosylated peptides, transient populations of folded conformations are present. The restricted rotation of α-D-GalpNAc at Thr-10 around the linkage between the glycan and the peptide was explained by intramolecular hydrogen bonding between the amide proton of GalNAc and most likely the carbonyl oxygen of Thr-10 in the peptide chain. Such a hydrogen bond was not seen for the peptide O-glycosylated at Ser-7. A comparison of the activities of the four compounds in an assay of acute pain (ability to induce latency of tail flick in mice) demonstrated that a reduction of the size of the glycan, or a shift in the position of the glycosylation site, decreases the activity with respect to contulakin-G itself. Therefore, it was suggested that the stabilization of the peptide conformation by hydrogen bonding to the carbohydrate could be a key factor in the biological activity . In this context, it should be noted that the T-antigen at Thr-10 showed significant protection against enzymatic degradation by Pro-specific endopeptidase, but when attached to Ser-7, this protection was completely abolished. Based on these data, it was hypothesized that it is the orientation of the glycan chain relative to the peptide chain that is actually recognized by the proteolytic enzyme .
In a subsequent study, the NMR solution conformations of the [L-Ser-10] and [D-Ser-10] analogs of contulakin-G were reported , and subtle differences in conformational preferences between the analogs and native contulakin-G were found. In fact, the intramolecular hydrogen bonding as occurring in native contulakin-G was lacking. Interestingly, the biological activity of the [D-Ser-10] analog of contulakin-G was similar to that of contulakin-G itself. Thus the hydrogen bond between the glycan and the peptide in contulakin-G seems not to direct the biological activity. The [L-Ser-10] analog showed some activity at more than 100 times the dose.
In another study, for direct comparison with contulakin-G comprising β-D-Galp-(1→3)-α-D-GalpNAc-(1→O)- at Thr-10, three analogs with different O-glycans at Thr-10, i.e., β-D-Galp-(1→3)-β-D-GalpNAc-(1→O)-, α-D-Galp-(1→3)-α-D-GalpNAc-(1→O)-, and β-D-Galp-(1→6)-α-D-GalpNAc-(1→O)-, respectively, were synthesized  (see Scheme 2), but so far biological and conformational details are missing.
A study focusing on the enzymatic glycosylation of the non-glycosylated form of contulakin-G showed that the mammalian UDP-D-GalNAc:polypeptide:α-GalNAc-transferase T1 (ppGalNAc-transferase T1, EC 18.104.22.168) was able to transfer GalNAc from UDP-GalNAc to Thr-10 of the peptide backbone, although Ser-7 was also glycosylated to some extent . It is not clear if this glycosylated product was tested for bioactivity.
4.3. Conus textile
The glycosylated conotoxin ε-TxIX (also called tx5a, Tx-012 or TxVa), with a backbone of 13 amino acids, occurs as the most abundant peptide in the venom of the mollusc-hunting cone snail C. textile. Nine out of the thirteen amino acids are post-translationally modified . The post-translational modifications comprise: γ-carboxyglutamic acid, 6-bromotryptophan, 4-trans-hydroxyproline at the C-terminus, and O-glycosylation at Thr-10, in addition to two disulfide bridges (Table 2). When injected intracerebroventricularly into mice, it causes hyperactivity and spasticity. It is suggested that the glycopeptide may target presynaptic calcium channels (blocker) or act on G protein-coupled presynaptic receptors via another mechanism . Using monosaccharide analysis and MALDI-TOF-MS, the O-glycan was defined as a disaccharide Gal-GalNAc. Despite no reference to an analysis of the linkage types and anomericities of the carbohydrate constituents, the solution structure of ε-TxIX was determined by NMR spectroscopy and showed a high flexibility of the disaccharide moiety .
In a parallel report, the same structure (as Hex-HexNAc) was presented, as determined by MALDI-TOF-MS and ESI-MS/MS . The Hex and HexNAc residues were identified to be galactose and N-acetylgalactosamine, but no linkage type was reported.
Detailed structural information on ε-TxIX, obtained by 1D and 2D NMR (COSY, HSQC, NOESY) spectroscopy , yielded a core-8 type structure, α-D-Galp-(1→3)-α-D-GalpNAc-(1→O)- (see Scheme 2). Note that the anomeric configuration of the terminal galactose residue (α) is the only difference with the O-glycan structure (T-antigen; see Scheme 1) determined for conopeptide contulakin-G from Conus geographus venom. The inability to split off terminal galactose using β-galactosidase inferred the presence of terminal α-galactose in tx5a. Likewise, the inability to split off the disaccharide moiety with endo-O-glycosidase (endo-α-N-acetylgalactosaminidase) inferred interglycosidic linkages distinct from those found in the T-antigen . However, the absolute configuration (D) of the monosaccharide constituents was not actually determined.
4.4. Conus magus
κA-conotoxin MIVA (κA-MIVA), the 36-amino acid peptide from the venom of the fish-hunting species C. magus, causes the same spastic symptomatology as κA-SIVA . The conopeptide contains three disulfide bonds and has ten post-translationally modified amino acids. These include seven hydroxylated proline residues, including one C-terminal, a γ-carboxy-glutaminic acid and two modified threonine residues (may be O-glycosylated), i.e., Thr-7 and Thr-9 . The amino acid sequence of the conopeptide is depicted in Table 2. O-Glycan details were not included for κA-MIVA, but we noted that the mass difference (1053.6 Da) found in LSI-MS studies of the native glycopeptide as compared to the unglycosylated form agrees with a composition Hex4HexNAc2 shared between the two glycosylation sites.
4.5. Conus stercusmuscarum
The 37-amino acid κA-conotoxins SmIVA and SmIVB from the venom of the Indo-Pacific fish-hunting species C. stercusmuscarum elicit a spastic paralysis upon injection of venom into the fish during prey capture . Both compounds were suggested to be O-glycosylated at Ser-7 and Thr-8, but details about the glycans present have not been published. The predicted mature toxin primary structure is included in Table 2.
4.6. Conus consors
The conotoxin CcTx, isolated from the venom of the Indo-Pacific fish-hunting cone snail C. consors (Pionoconus clade) produced a marked contraction and extension of the caudal and dorsal fins upon injection into fish . When tested on isolated frog neuromuscular preparations, CcTx showed skeletal muscle contractions, indicating a potent excitotoxin that targets tetrodotoxin-sensitive voltage-gated sodium channels. It selectively increases motor nerve terminal excitability resulting in repetitive and spontaneous action potential that lead to sudden titanic paralysis of the prey. The conopeptide belongs to the κA-family of conotoxins, having 73% sequence homology with κA-SIVA (from C. striatus venom) and the same cysteine scaffold .
Chemical microsequencing and ESI-MS revealed a peptide of 30 amino acids with three disulfide bridges, a C-terminal cysteine residue and three 4-hydroxyproline residues [15,58]. A post-translational modification involving an O-glycosylation of a Ser or Thr residue at position 7 was suggested. Additional studies on the composition of dissected venom versus milked venom of C. consors yielded CcTx as a major compound in both sources. Using RP-HPLC followed by MALDI-TOF-MS and ESI-MS, the O-glycosylation site was fixed at Ser-7, although the composition of the O-glycan was not determined yet (Table 2) . Besides CcTx (MW = 4118.2 Da), a second major compound of a higher molecular mass (5179.7 Da) was detected in the venom, specified as an unknown glycosylated peptide (“CcTx-like”) . Furthermore, a partially deglycosylated CcTx component (3953.7 Da), missing one Hex residue (162 Da), was observed. The latter long-term study demonstrated that the injected venoms of C. consors individuals are not constant in peptide composition and can drastically vary with time.
Recently, a detailed investigation of the O-glycan of CcTx has been reported . Using MALDI-TOF-MS and ESI-MS the carbohydrate chain at Ser-7 could be described as Hex3HexNAc2. Using monosaccharide analysis, absolute configuration determination, methylation analysis and NMR spectroscopy, the complete structure for the O-glycan chain of CcTx was determined to be α-L-Galp-(1→4)-α-D-GlcpNAc-(1→6)-[α-L-Galp-(1→2)-β-D-Galp-(1→3)-]α-D-GalpNAc-(1→O)-.
This O-glycan (see Scheme 3) has completely novel structural features. Besides a conventional β-D-Galp-(1→3)-α-D-GalpNAc-(1→O)- fragment, which also occurs in contulakin-G and in many mucin-type glycosylations, the α-D-GalpNAc- unit is substituted at O6 with an α-D-GlcpNAc-(1→6)- unit, yielding a novel core-type structure α-D-GlcpNAc-(1→6)-[β-D-Galp-(1→3)-]α-D-GalpNAc-(1→O)-, which was defined as core-type 9. However, the most remarkable finding was the occurrence of terminal α-Galp- residues at the upper and lower branch, both having an L-configuration, which makes the O-glycan even more unique. Analysis of the NMR solution structure of CcTx (Figure 2) showed that the backbone of the C-terminal region was well defined with three disulfide bridges, a series of turns, including a Type I’ β-turn for Cys-12–Tyr-15 and a partially distorted Type I β-turn for Asn-16–Thr-19, preceding the short α-helix Ser-23–Thr-27.
The N-terminal region Ala-1–Thr-11 appears less well defined, but presents a kink in the backbone centered on the O-glycosylated Ser-7. Although the N-terminal region is more disordered, the pentasaccharide orients itself consistently on one side of the peptide, with the α-D-GalpNAc and, to a lesser extent, the α-D-GlcpNAc residue oriented towards the peptide chain .
5. Peptide Sequence Comparison
Unlike the Asn-Xxx-Ser/Thr consensus amino acid sequence for N-glycosylation, no consensus target sequence has been identified for O-glycosylation of proteins/peptides , let alone that of conotoxins. However, an interesting pattern has emerged from the comparison of the peptide sequences for members of the κA-conotoxin family . These sequences, known and predicted from cDNA, can be divided into three groups, each distinct from one other in terms of level of glycosylation. The first group comprises the κA-conopeptides that have an O-glycosylated serine residue at position 7 in their respective sequences. The conotoxins CcTx (C. consors), κA-SIVA (C. striatus), κA-SIVB (C. striatus), SmIVA (C. stercusmuscarum), and SmIVB (C. stercusmuscarum) belong to this group. As shown in Table 2, there is a significant peptide sequence identity between these five compounds. The closest homolog to CcTx is κA-SIVA, showing 73% sequence identity. As previously mentioned, CcTx, κA-SIVA, and κA-SIVB have been shown to contain an O-glycan with the composition Hex3HexNAc2 and for CcTx the carbohydrate structure has been elucidated in detail. We suggest that the O-glycans of κA-SIVA and κA-SIVB could have the same unusual primary structure as found for CcTx. The second group comprises the κA-conopeptides that are predicted to have two glycosylated threonine residues at position 7 and 9, as demonstrated for κA-MIVA (C. magus). So far, structural details of the O-glycan are missing. The third group, known as short κA-conotoxins, lacks the O-glycosylated N-terminal tail present in the other two groups.
Contulakin-G (C. geographus) and ε-TxIX (C. textile) are the smallest conotoxins and contain no sequence similarity to other conopeptides as shown in Table 2, except that they both have a glycosylated threonine residue at position 10. However, their O-glycan disaccharides differ in the anomeric configuration (β versus α, respectively) of the non-reducing D-Galp residue (vide supra and Table 2).
6. Biosynthesis and Roles of O-Glycosylation in Conotoxins
Several recent reviews are available in the literature describing the current knowledge of the peptide biosynthesis of conotoxins [42,71,72,73,74]. As mentioned already in the Introduction, many constituent amino acids of the peptides are post-translationally modified. The biosynthesis of the many different conotoxins is probably associated with the specific type of epithelial cells found in different sections of the venom duct of the snail. For instance, qualitative and quantitative differences in conotoxin components were found in the proximal, central and distal sections of the C. textile and C. geographus venom duct, suggesting specialization of duct sections for the biosynthesis of particular conotoxins [75,76,77]. However, most biochemical and cellular events that occur in the venom duct have not yet been fully characterized.
Although the pathways of O-glycosylation for conotoxins have not been outlined in detail, the observation that the synthetic non-glycosylated contulakin-G peptide could be glycosylated by a mammalian UDP-D-GalNAc: polypeptide α-GalNAc-transferase (i.e., ppGalNAcT1) ) points to a similar pathway for O-glycosylation in Conus species as in mammals. This means that all monosaccharides are added one at a time in a stepwise series of reactions, in contrast to the formation of a lipid-linked precursor oligosaccharide followed by en bloc transfer of the oligosaccharide to the polypeptide in N-glycosylation. The O-glycosylation starts with the transfer of D-GalpNAc from UDP-α-D-GalpNAc to a Ser or Thr residue of the peptide backbone. The attachment of α-D-GalpNAc is catalyzed by one of the members of the large ppGalNAcT family, yielding the α-D-GalpNAc-(1→O)-Ser/Thr element [49,50]. As mentioned before, there are no simple peptide target sequences for O-glycosylation, analogous to the Asn-Xxx-Ser/Thr sequences for N-glycosylation, and it has to be noted that ppGalNAcTs differ in their specificity for different sequences of amino acids surrounding the glycosylation target. However, a preponderance of adjacent Pro and Ala residues has been associated with sites of O-glycosylation . Pro residues appear to influence protein conformation by breaking helix formation and promoting the formation of β-turns and β-sheets. In subsequent reactions, additional monosaccharides are transferred individually from nucleotide sugar donors to the growing O-glycan chain by a variety of glycosyltransferases. The core-1 type disaccharide β-D-Galp-(1→3)-α-D-GalpNAc, as found in contulakin-G (C. geographus), is the major core type found in mammals, and its biosynthesis using mammalian β-1,3-D-galactosyltransferase (core 1 β3GalT) has been well described . A similar transferase is expected to be present in C. geographus. For the core-8 type disaccharide α-D-Galp-(1→3)-α-D-GalpNAc, present in ε-TxIX (C. textile), and found earlier in human bronchial tissues , no gene potentially encoding this α-1,3-D-galactosyltransferase has been identified. However, such an enzyme is well known for the biosynthesis of the Galili epitope α-D-Galp-(1→3)-β-D-Galp-(1→4)-β-D-GlcpNAc(1→ [79,80,81]. The recently identified novel core-9 type fragment α-D-GlcpNAc-(1→6)-[β-D-Galp-(1→3)-]α-D-GalpNAc in the pentasaccharide O-glycan of CcTx of C. consors  would require an α-1,6-N-acetyl-D-glucosaminyltransferase, an enzyme that also has not yet been described. Moreover, for the biosynthesis of the complete pentasaccharide in CcTx, two other unknown glycosyltransferases are needed, namely, α-1,4-L-galactosyltransferase and α-1,2-L-galactosyltransferase, as well as the nucleotide L-Galp donor.
It is important to consider the biological significance of these complex carbohydrates of conotoxins. The biological functions of glycoprotein/glycopeptide glycans can be roughly divided into two broad categories: (1) intrinsic functions performed by glycans, such as providing structural components and modifying physiological properties; (2) extrinsic functions resulting from glycan–protein or glycan–glycan interactions, such as directing trafficking, mediating and modulating cell-adhesion and signaling [82,83]. In the case of conotoxins, it has been reported that the post-translational modifications of amino acids increased the toxin potency [36,38,42,72,84] and assisted the stabilization of the three-dimensional molecular structure [60,66,85]. This fits the general agreement that the glycosylation of peptides may increase their biological stability by slowing down the proteolytic degradation of the polypeptide backbones (as a means of protection), as well as by stabilizing their tertiary structures, thereby increasing their lifespans. Nevertheless, in the whole array of biological events, carbohydrate-recognizing receptors and lysosomal catabolic enzymes might also play an important role. As mentioned earlier, it was speculated that the role of the O-glycan (T-antigen) in contulakin-G could be to increase the stability in vivo, thereby enhancing the bioavailability of the toxin at the receptor site [36,54,59,61]. With regards to lysosomal catabolism, it should be noted that relatively little is known about O-glycans in comparison with N-glycans [86,87]. O-Glycosylation could be responsible for enhancing the stability of CcTx (C. consors) in vivo and the presence of terminal α-L-galactose residues in the upper and lower branch of the glycan chain (Scheme 3) may further enhance this stability. Indeed, glycoconjugates generally contain D-galactose residues, which can be released with conventional D-galactosidases in catabolic pathways. This would mean that in preys devoid of L-galactosidases, the presence of terminal α-L-galactose could provide an extra level of protection against the breakdown of CcTx. However, to the best of our knowledge, the presence of α-L-galactosidases in fish, being the preys of C. consors, has not been reported. It is, however, known that marine microorganisms can express α-1,3-(3,6-anhydro)-L-galactosidase and α-1,3-(6-sulfate)-L-galactosidase, enzymes that catalyze the degradation of agar, the major component of the cell walls of red algae [88,89]. But the roles served by glycosylation and many other post-translational modifications in conotoxins remain, for the most part, unexplored. In higher mammals, α-L-galactosidases have not been reported, which is of interest, considering the potential applications of these conotoxins in the treatment of human neurological disorders.
It is clear that the study of the biological role of the glycan in conotoxins requires an unambiguous determination of the identity and quantity of the glycan species. Determining the 3D structure of the complete peptides, elucidated using NMR and molecular dynamics, is crucial to our understanding of the structure–activity relationship of these peptides [52,85,90,91].
7. Concluding Remarks
Cone snail venom will continue to attract a growing interest as a vast untapped biological resource [92,93]. Conotoxins have proven effective in drug design and could be used to treat various disorders such as schizophrenia, neuromuscular disorders, and chronic pain. Nowadays, the venoms of more than 500 species of cone snails are being systematically characterized. This is an overwhelming task because each Conus species contains hundreds of peptides in its venom, and, overall, these peptides exhibit high amino acid sequence diversity, both between species and within species [9,11,90,94]. Moreover, it has been observed that the venom variations between individuals of a single geographical population are greater than variations observed between geographical distant populations. The significant inter- and intra-species differences in the venom repertoire constitute an intriguing challenge for scientists investigating the proteins involved in biosynthesis, modification and secretion of such an enormous diversity of compounds (a field called “Venomics”) [95,96]. The current knowledge of the glycosylation of conopeptides has been summarized in Table 2. Thus far, it seems that glycosylation occurs mostly in fish-hunting species.
We have observed that, since the discovery of a possible glycosylation of conotoxins, little or no attention has been paid to the detailed structural analysis of the conopeptide glycans. One can only speculate about the reasons why this is the case, but perhaps this aspect has simply been overlooked. It is hoped that this review will contribute to an increase of glycobiology activities in the venomics field.
This work has been supported by a grant from the European Commission: CONCO, the cone snail genome project for health, Integrated Project ref. LSHB-CT-2007-037592 (http://www.conco.eu). We would like to thank our scientific officer Torbjörn Ingemansson, the government of New Caledonia, and the French Institute for Research and Development (IRD) for their help and support. We thank Conchology Inc. for permission to use their photographic material.
- Conchology Inc. Available online: http://www.conchology.be (accessed on 5 December 2012).
- CONCO, the Cone Snail Genome Project for Health. Available online: http://www.conco.eu/ (accessed on 5 December 2012).
- Terlau, H.; Shon, K.J.; Grilley, M.; Stocker, M.; Stühmer, W.; Olivera, B.M. Strategy for rapid immobilization of prey by a fish-hunting marine snail. Nature 1996, 381, 148–151.
- West, D.J.; Andrews, E.B.; Bowman, D.; McVean, A.R.; Thorndyke, M.C. Toxins from some poisonous and venomous marine snails. Comp. Biochem. Phys.Part C 1996, 113, 1–10.
- Olivera, B.M.; Rivier, J.; Clark, C.; Ramilo, C.A.; Corpuz, G.P.; Abogadie, F.C.; Mena, E.E.; Woodward, S.R.; Hillyard, D.R.; Cruz, L.J. Diversity of Conus neuropeptides. Science 1990, 249, 257–263.
- Rockel, D.; Korn, W.; Kohn, A.J. Manual of the Living Conidae; Verlag Christa Hemmen: Wiesbaden, Germany, 1995.
- Espiritu, D.J.; Watkins, M.; Dia-Monje, V.; Cartier, G.E.; Cruz, L.J.; Olivera, B.M. Venomous cone snails: Molecular phylogeny and the generation of toxin diversity. Toxicon 2001, 39, 1899–1916, doi:10.1016/S0041-0101(01)00175-1.
- Kaas, Q.; Westermann, J.-C.; Craik, D.J. Conopeptide characterization and classifications: An analysis using ConoServer. Toxicon 2010, 55, 1491–1509, doi:10.1016/j.toxicon.2010.03.002.
- Davis, J.; Jones, A.; Lewis, R.J. Remarkable inter- and intra-species complexity of conotoxins revealed by LC/MS. Peptides 2009, 30, 1222–1227, doi:10.1016/j.peptides.2009.03.019.
- Biass, D.; Dutertre, S.; Gerbault, A.; Menou, J.-L.; Offord, R.; Favreau, P.; Stöcklin, R. Comparative proteomic study of the venom of the piscivorous cone snail Conus consors. J. Proteomics 2009, 72, 210–218, doi:10.1016/j.jprot.2009.01.019.
- Dutertre, S.; Biass, D.; Stöcklin, R.; Favreau, P. Dramatic intraspecimen variations within the injected venom of Conus consors: An unsuspected contribution to venom diversity. Toxicon 2010, 55, 1453–1462, doi:10.1016/j.toxicon.2010.02.025.
- Dutertre, S.; Jin, A.-H.; Kaas, Q.; Jones, A.; Alewood, P.F.; Lewis, R.J. Deep venomics reveals the mechanism for expanded peptide diversity in cone snail venom. Mol. Cell. Proteomics 2012, 12, 312–329.
- Violette, A.; Biass, D.; Dutertre, S.; Koua, D.; Piquemal, D.; Pierrat, F.; Stöcklin, R.; Favreau, P. Large-scale discovery of conopeptides and conoproteins in the injectable venom of a fish-hunting cone snail using a combined proteomic and transcriptomic approach. J. Proteomics 2012, 75, 5215–5225, doi:10.1016/j.jprot.2012.06.001.
- Leonardi, A.; Biass, D.; Kordiš, D.; Stöcklin, R.; Favreau, P.; Križaj, I. Conus consors snail venom proteomics proposes functions, pathways, and novel families involved in its venomic system. J. Proteome Res. 2012, 11, 5046–5058, doi:10.1021/pr3006155.
- Le Gall, F.; Favreau, P.; Richard, G.; Letourneux, Y.; Molgó, J. The strategy used by some piscivorous cone snails to capture their prey: The effects of their venoms on vertebrates and on isolated neuromuscular preparations. Toxicon 1999, 37, 985–998, doi:10.1016/S0041-0101(98)00227-X.
- Yoshiba, S. An estimation of the most dangerous species of cone shell, Conus (Gastridium) geographus Linne, 1758, venom’s lethal dose in humans. Nihon Eiseigaku Zasshi 1984, 39, 565–572, doi:10.1265/jjh.39.565.
- Fegan, D.; Andresen, D. Conus geographus envenomation. Lancet 1997, 349, 1672, doi:10.1016/S0140-6736(05)62639-6.
- McIntosh, J.M.; Jones, R.M. Cone venom—From accidental stings to deliberate injection. Toxicon 2001, 39, 1447–1451, doi:10.1016/S0041-0101(01)00145-3.
- Jones, R.M.; Bulaj, G. Conotoxins—New vistas for peptide therapeutics. Curr. Pharm. Des. 2000, 6, 1249–1285, doi:10.2174/1381612003399653.
- Lewis, R.J.; Garcia, M.L. Therapeutic potential of venom peptides. Nat. Rev. Drug Discov. 2003, 2, 790–802, doi:10.1038/nrd1197.
- Terlau, H.; Olivera, B.M. Conus venoms: A rich source of novel ion channel-targeted peptides. Physiol. Rev. 2004, 84, 41–68, doi:10.1152/physrev.00020.2003.
- Han, T.S.; Teichert, R.W.; Olivera, B.M.; Bulaj, G. Conus venoms—A rich source of peptide-based therapeutics. Curr. Pharm. Des. 2008, 14, 2462–2479, doi:10.2174/138161208785777469.
- Favreau, P.; Stöcklin, R. Marine snail venoms: Use and trends in receptor and channel neuropharmacology. Curr. Opin. Pharmacol. 2009, 9, 594–601, doi:10.1016/j.coph.2009.05.006.
- Molinski, T.F.; Dalisay, D.S.; Lievens, S.L.; Saludes, J.P. Drug development from marine natural products. Nat. Rev. Drug Discov. 2009, 8, 69–85.
- Lewis, R.J.; Dutertre, S.; Vetter, I.; Christie, M.J. Conus venom peptide pharmacology. Pharmacol. Rev. 2012, 64, 259–298, doi:10.1124/pr.111.005322.
- Livett, B.G.; Gayler, K.R.; Khalil, Z. Drugs from the sea: Conopeptides as potential therapeutics. Curr. Med. Chem. 2004, 11, 1715–1723, doi:10.2174/0929867043364928.
- Fox, J.W.; Serrano, S.M.T. Approaching the golden age of natural product pharmaceuticals from venom libraries: An overview of toxins and toxin-derivatives currently involved in therapeutic or diagnostic applications. Curr. Pharm. Des. 2007, 13, 2927–2934, doi:10.2174/138161207782023739.
- Bingham, J.-P.; Mitsunaga, E.; Bergeron, Z.L. Drugs from slugs—Past, present and future perspectives of ω-conotoxin research. Chem. Biol. Interact. 2010, 183, 1–18, doi:10.1016/j.cbi.2009.09.021.
- Essack, M.; Bajic, V.B.; Archer, J.A.C. Conotoxins that confer therapeutic possibilities. Mar. Drugs 2012, 10, 1244–1265, doi:10.3390/md10061244.
- Bingham, J.-P.; Andrews, E.A.; Kiyabu, S.M.; Cabalteja, C.C. Drugs from slugs. Part II—Conopeptide bioengineering. Chem. Biol. Interact. 2012, 200, 92–113, doi:10.1016/j.cbi.2012.09.021.
- Miljanich, G.P. Ziconotide: Neuronal calcium channel blocker for treating severe chronic pain. Curr. Med. Chem. 2004, 11, 3029–3040, doi:10.2174/0929867043363884.
- Staats, P.S.; Yearwood, T.; Charapata, S.G.; Presley, R.W.; Wallace, M.S.; Byas-Smith, M.; Fisher, R.; Bryce, D.A.; Mangieri, E.A.; Luther, R.R.; et al. Intrathecal ziconotide in the treatment of refractory pain in patients with cancer or AIDS: A randomized controlled trial. JAMA 2004, 291, 63–70.
- Williams, J.A.; Day, M.; Heavner, J.E. Ziconotide: An update and review. Expert Opin. Pharmacother. 2008, 9, 1575–1583, doi:10.1517/14656522.214.171.1245.
- Shen, G.S.; Layer, R.T.; McCabe, R.T. Conopeptides: From deadly venoms to novel therapeutics. Drug Discov. Today 2000, 5, 98–106, doi:10.1016/S1359-6446(99)01454-3.
- Daly, N.L.; Rosengren, K.J.; Henriques, S.T.; Craik, D.J. NMR and protein structure in drug design: Application to cyclotides and conotoxins. Eur. Biophys. J. 2011, 40, 359–370, doi:10.1007/s00249-011-0672-9.
- Craig, A.G.; Bandyopadhyay, P.; Olivera, B.M. Post-translationally modified neuropeptides from Conus venoms. Eur. J. Biochem. 1999, 264, 271–275, doi:10.1046/j.1432-1327.1999.00624.x.
- Arias, H.R.; Blanton, M.P. α-Conotoxins. Int. J. Biochem. Cell Biol. 2000, 32, 1017–1028, doi:10.1016/S1357-2725(00)00051-0.
- Craig, A.G. The characterisation of conotoxins. J. Toxicol. Toxin Rev. 2000, 19, 53–93, doi:10.1081/TXR-100100315.
- Olivera, B.M.; Cruz, L.J. Conotoxins, in retrospect. Toxicon 2001, 39, 7–14, doi:10.1016/S0041-0101(00)00157-4.
- Conticello, S.G.; Gilad, Y.; Avidan, N.; Ben-Asher, E.; Levy, Z.; Fainzilber, M. Mechanisms for evolving hypervariability: The case of conopeptides. Mol. Biol. Evol. 2001, 18, 120–131.
- Wang, C.-Z.; Chi, C.-W. Conus peptides—A rich pharmaceutical treasure. Acta Biochim. Biophys. Sin. (Shanghai) 2004, 36, 713–723, doi:10.1093/abbs/36.11.713.
- Buczek, O.; Bulaj, G.; Olivera, B.M. Conotoxins and the posttranslational modification of secreted gene products. Cell. Mol. Life Sci. 2005, 62, 3067–3079, doi:10.1007/s00018-005-5283-0.
- Olivera, B.M. Conus peptides: Biodiversity-based discovery and exogenomics. J. Biol. Chem. 2006, 281, 31173–31177, doi:10.1074/jbc.R600020200.
- Norton, R.S.; Olivera, B.M. Conotoxins down under. Toxicon 2006, 48, 780–798, doi:10.1016/j.toxicon.2006.07.022.
- Olivera, B.M.; Teichert, R.W. Diversity of the neurotoxic Conus peptides: A model for concerted pharmacological discovery. Mol. Interv. 2007, 7, 251–260, doi:10.1124/mi.7.5.7.
- Halai, R.; Craik, D.J. Conotoxins: Natural product drug leads. Nat. Prod. Rep. 2009, 26, 526–536, doi:10.1039/b819311h.
- Kaas, Q.; Westermann, J.-C.; Halai, R.; Wang, C.K.L.; Craik, D.J. ConoServer, a database for conopeptide sequences and structures. Bioinformatics 2007, 24, 445–446.
- Kaas, Q.; Yu, R.; Jin, A.-H.; Dutertre, S.; Craik, D.J. ConoServer: Updated content, knowledge, and discovery tools in the conopeptide database. Nucl. Acids Res. 2012, 40, D325–D330, doi:10.1093/nar/gkr886.
- Brockhausen, I.; Schachter, H.; Stanley, P. O-GalNAc Glycans. In Essentials of Glycobiology, 2nd; Varki, A., Cummings, R.D., Esko, J.D., Freeze, H.H., Stanley, P., Bertozzi, C.R., Hart, G.W., Etzler, M.E., Eds.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York, NY, USA, 2009; pp. 115–127.
- Brockhausen, I. Pathways of O-glycan biosynthesis in cancer cells. Biochim. Biophys. Acta 1999, 1473, 67–95, doi:10.1016/S0304-4165(99)00170-1.
- Vliegenthart, J.F.G.; Kamerling, J.P. 1H NMR Structural-Reporter-Group Concepts in Carbohydrate Analysis. In Comprehensive Glycoscience—From Chemistry to Systems Biology, 1st; Kamerling, J.P., Boons, G.-J., Lee, Y.C., Suzuki, A., Taniguchi, N., Voragen, A.G.J., Eds.; Elsevier: Oxford, UK, 2007; Volume 2, pp. 133–191.
- Hocking, H.G.; Gerwig, G.J.; Dutertre, S.; Violette, A.; Favreau, P.; Stöcklin, R.; Kamerling, J.P.; Boelens, R. Structure of the O-glycosylated conopeptide CcTx from Conus consors venom. Chem. Eur. J. 2013, 19, 870–879, doi:10.1002/chem.201202713.
- Kelley, W.P.; Schulz, J.R.; Jakubowski, J.A.; Gilly, W.F.; Sweedler, J.V. Two Toxins from Conus striatus that individually induce tetanic paralysis. Biochemistry 2006, 45, 14212–14222.
- Craig, A.G.; Zafaralla, G.; Cruz, L.J.; Santos, A.D.; Hillyard, D.R.; Dykert, J.; Rivier, J.E.; Gray, W.R.; Imperial, J.; DelaCruz, R.G.; et al. An O-glycosylated neuroexcitatory Conus peptide. Biochemistry 1998, 37, 16019–16025.
- Jakubowski, J.A.; Kelley, W.P.; Sweedler, J.V. Screening for post-translational modifications in conotoxins using liquid chromatography/mass spectrometry: An important component of conotoxin discovery. Toxicon 2006, 47, 688–699, doi:10.1016/j.toxicon.2006.01.021.
- Jakubowski, J.A.; Kelley, W.P.; Sweedler, J.V.; Gilly, W.F.; Schulz, J.R. Intraspecific variation of venom injected by fish-hunting Conus snails. J. Exp. Biol. 2005, 208, 2873–2883, doi:10.1242/jeb.01713.
- Santos, A.D.; McIntosh, J.M.; Hillyard, D.R.; Cruz, L.J.; Olivera, B.M. The A-superfamily of conotoxins: Structural and functional divergence. J. Biol. Chem. 2004, 279, 17596–17606.
- Le Gall, F.; Favreau, P.; Benoit, E.; Mattei, C.; Bouet, F.; Menou, J.-L.; Ménez, A.; Letourneux, Y.; Molgó, J. A new conotoxin isolated from Conus consors venom acting selectively on axons and motor nerve terminals through a Na+-dependent mechanism. Eur. J. Neurosci. 1999, 11, 3134–3142, doi:10.1046/j.1460-9568.1999.00732.x.
- Craig, A.G.; Norberg, T.; Griffin, D.; Hoeger, C.; Akhtar, M.; Schmidt, K.; Low, W.; Dykert, J.; Richelson, E.; Navarro, V.; et al. Contulakin-G, an O-glycosylated invertebrate neurotensin. J. Biol. Chem. 1999, 274, 13752–13759.
- Kindahl, L.; Sandström, C.; Craig, A.G.; Norberg, T.; Kenne, L. 1H NMR studies on the solution conformation of contulakin-G and analogues. Can. J. Chem. 2002, 80, 1022–1031, doi:10.1139/v02-115.
- Kindahl, L.; Kenne, L.; Sandström, C. 1H NMR studies on the solution conformation of the [L-Ser10] and [D-Ser10] analogues of contulakin-G. Can. J. Chem. 2005, 83, 156–165, doi:10.1139/v04-176.
- Westerlind, U.; Norberg, T. Chemical synthesis of analogs of the glycopeptide contulakin-G, an analgetically active conopeptide from Conus geographus. Carbohydr. Res. 2006, 341, 9–18, doi:10.1016/j.carres.2005.11.010.
- Craig, A.G.; Park, M.; Fischer, W.H.; Kang, J.; Compain, P.; Piller, F. Enzymatic glycosylation of contulakin-G, a glycopeptide isolated from Conus venom, with a mammalian ppGalNAc-transferase. Toxicon 2001, 39, 809–815, doi:10.1016/S0041-0101(00)00211-7.
- Rigby, A.C.; Lucas-Meunier, E.; Kalume, D.E.; Czerwiec, E.; Hambe, B.; Dahlqvist, I.; Fossier, P.; Baux, G.; Roepstorff, P.; Baleja, J.D.; et al. A conotoxin from Conus textile with unusual posttranslational modifications reduces presynaptic Ca2+ influx. Proc. Natl. Acad. Sci. USA 1999, 96, 5758–5763.
- Kalume, D.E.; Stenflo, J.; Czerwiec, E.; Hambe, B.; Furie, B.C.; Furie, B.; Roepstorff, P. Structure determination of two conotoxins from Conus textile by a combination of matrix-assisted laser desorption/ionization time-of-flight and electrospray ionization mass spectrometry and biochemical methods. J. Mass Spectrom. 2000, 35, 145–156, doi:10.1002/(SICI)1096-9888(200002)35:2<145::AID-JMS922>3.0.CO;2-I.
- Kang, J.; Low, W.; Norberg, T.; Meisenhelder, J.; Hansson, K.; Stenflo, J.; Zhou, G.-P.; Imperial, J.; Olivera, B.M.; Rigby, A.C.; et al. Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure is α-D-Gal-(1→3)-α-D-GalNAc. Eur. J. Biochem. 2004, 271, 4939–4949, doi:10.1111/j.1432-1033.2004.04464.x.
- Kern, S.; Allen, J.; Wagstaff, J.; Shafer, S.L.; Yaksh, T. The pharmacokinetics of the conopeptide contulakin-G (CGX-1160) after intrathecal administration: An analysis of data from studies in beagles. Anesth. Analg. 2007, 104, 1514–1520, doi:10.1213/01.ane.0000262038.58546.e6.
- Allen, J.W.; Hofer, K.; McCumber, D.; Wagstaff, J.D.; Layer, R.T.; McCabe, R.T.; Yaksh, T.L. An assessment of the antinociceptive efficacy of intrathecal and epidural contulakin-G in rats and dogs. Anesth. Analg. 2007, 104, 1505–1513, doi:10.1213/01.ANE.0000219586.65112.FA.
- Jones, R.M.; Bulaj, G. Conus peptides—Combinatorial chemistry at a cone snail’s pace. Curr. Opin. Drug Discov. Dev. 2000, 3, 141–154.
- NetOGlyc 3.1 Server. Available online: http://www.cbs.dtu.dk/services/NetOGlyc (accessed on 5 December 2012).
- Garrett, J.E.; Buczek, O.; Watkins, M.; Olivera, B.M.; Bulaj, G. Biochemical and gene expression analyses of conotoxins in Conus textile venom ducts. Biochem. Biophys. Res. Commun. 2005, 328, 362–367, doi:10.1016/j.bbrc.2004.12.178.
- Bulaj, G.; Olivera, B.M. Folding of conotoxins: Formation of the native disulfide bridges during chemical synthesis and biosynthesis of Conus peptides. Antioxid. Redox Signal. 2008, 10, 141–155, doi:10.1089/ars.2007.1856.
- Safavi-Hemami, H.; Siero, W.A.; Gorasia, D.G.; Young, N.D.; Macmillan, D.; Williamson, N.A.; Purcell, A.W. Specialisation of the venom gland proteome in predatory cone snails reveals functional diversification of the conotoxin biosynthetic pathway. J. Proteome Res. 2011, 10, 3904–3919, doi:10.1021/pr1012976.
- Safavi-Hemami, H.; Gorasia, D.G.; Steiner, A.M.; Williamson, N.A.; Karas, J.A.; Gajewiak, J.; Olivera, B.M.; Bulaj, G.; Purcell, A.W. Modulation of conotoxin structure and function is achieved through a multienzyme complex in the venom glands of cone snails. J. Biol. Chem. 2012, 287, 34288–34303.
- Tayo, L.L.; Lu, B.; Cruz, L.J.; Yates, J.R., III. Proteomic analysis provides insights on venom processing in Conus textile. J. Proteome Res. 2010, 9, 2292–2301, doi:10.1021/pr901032r.
- Hu, H.; Bandyopadhyay, P.; Olivera, B.; Yandell, M. Elucidation of the molecular envenomation strategy of the cone snail Conus geographus through transcriptome sequencing of its venom duct. BMC Genomics 2012, 13, 284, doi:10.1186/1471-2164-13-284.
- Dobson, R.; Collodoro, M.; Gilles, N.; Turtoi, A.; de Pauw, E.; Quinton, L. Secretion and maturation of conotoxins in the venom ducts of Conus textile. Toxicon 2012, 60, 1370–1379, doi:10.1016/j.toxicon.2012.09.013.
- Van Halbeek, H.; Strang, A.M.; Lhermitte, M.; Rahmoune, H.; Lamblin, G.; Roussel, P. Structures of monosialyl oligosaccharides isolated from the respiratory mucins of a non-secretor (O, Lea+b−) patient suffering from chronic bronchitis. Characterization of a novel type of mucin carbohydrate core structure. Glycobiology 1994, 4, 203–219, doi:10.1093/glycob/4.2.203.
- Dorland, L.; van Halbeek, H.; Vliegenthart, J.F.G. The identification of terminal α(1→3)-linked galactose in N-acetyllactosamine type of glycopeptides by means of 500-MHz 1H-NMR spectroscopy. Biochem. Biophys. Res. Commun. 1984, 122, 859–866, doi:10.1016/S0006-291X(84)80113-8.
- Joziasse, D.H.; Shaper, J.H.; van den Eijnden, D.H.; van Tunen, A.J.; Shaper, N.L. Bovine α1→3-galactosyltransferase: Isolation and characterization of a cDNA clone. Identification of homologous sequences in human genomic DNA. J. Biol. Chem. 1989, 264, 14290–14297.
- Joziasse, D.H.; Shaper, N.L.; Kim, D.; van den Eijnden, D.H.; Shaper, J.H. Murine α1,3-galactosyltransferase. A single gene locus specifies four isoforms of the enzyme by alternative splicing. J. Biol. Chem. 1992, 267, 5534–5541.
- Varki, A.; Lowe, J.B. Biological roles of glycans. In Essentials of Glycobiology, 2nd; Varki, A., Cummings, R.D., Esko, J.D., Freeze, H.H., Stanley, P., Bertozzi, C.R., Hart, G.W., Etzler, M.E., Eds.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York, NY, USA, 2009; pp. 75–88.
- Taylor, M.E.; Drickamer, K. Introduction to Glycobiology, 3rd ed.; Oxford University Press: Oxford, UK, 2011; p. 4.
- Lopez-Vera, E.; Walewska, A.; Skalicky, J.J.; Olivera, B.M.; Bulaj, G. Role of hydroxyprolines in the in vitro oxidative folding and biological activity of conotoxins. Biochemistry 2008, 47, 1741–1751, doi:10.1021/bi701934m.
- Marx, U.C.; Daly, N.L.; Craik, D.J. NMR of conotoxins: Structural features and an analysis of chemical shifts of post-translationally modified amino acids. Magn. Reson. Chem. 2006, 44, S41–S50, doi:10.1002/mrc.1821.
- Winchester, B. Lysosomal metabolism of glycoproteins. Glycobiology 2005, 15, 1R–15R, doi:10.1093/glycob/cwi041.
- Freeze, H.H. Genetic Disorders of Glycan Degradation. In Essentials of Glycobiology, 2nd; Varki, A., Cummings, R.D., Esko, J.D., Freeze, H.H., Stanley, P., Bertozzi, C.R., Hart, G.W., Etzler, M.E., Eds.; Cold Spring Harbor Laboratory Press: Cold Spring Harbor, New York, NY, USA, 2009; pp. 567–600.
- Rebuffet, E.; Groisillier, A.; Thompson, A.; Jeudy, A.; Barbeyron, T.; Czjzek, M.; Michel, G. Discovery and structural characterization of a novel glycosidase family of marine origin. Environ. Microbiol. 2011, 13, 1253–1270, doi:10.1111/j.1462-2920.2011.02426.x.
- Chi, W.-J.; Chang, Y.-K.; Hong, S.-K. Agar degradation by microorganisms and agar-degrading enzymes. Appl. Microbiol. Biotechnol. 2012, 94, 917–930, doi:10.1007/s00253-012-4023-2.
- Romeo, C.; Di Francesco, L.; Oliverio, M.; Palazzo, P.; Massilia, G.R.; Ascenzi, P.; Polticelli, F.; Schininà, M.E. Conus ventricosus venom peptides profiling by HPLC-MS: A new insight in the intraspecific variation. J. Sep. Sci. 2008, 31, 488–498.
- Rosengren, K.J.; Daly, N.L.; Craik, D.J. NMR of peptide toxins. Annu. Rep. NMR Spectrosc. 2009, 68, 89–147, doi:10.1016/S0066-4103(09)06802-1.
- Blunt, J.W.; Copp, B.R.; Keyzers, R.A.; Munro, M.H.G.; Prinsep, M.R. Marine natural products. Nat. Prod. Rep. 2012, 29, 144–222, doi:10.1039/c2np00090c.
- Favreau, P.; Benoit, E.; Hocking, H.G.; Carlier, L.; D’ hoedt, D.; Leipold, E.; Markgraf, R.; Schlumberger, S.; Córdova, M.A.; Gaertner, H.; et al. A novel μ-conopeptide, CnIIIC, exerts potent and preferential inhibition of Nav1.2/1.4 channels and blocks neuronal nicotinic acetylcholine receptors. Br. J. Pharmacol. 2012, 166, 1654–1668, doi:10.1111/j.1476-5381.2012.01837.x.
- Rivera-Ortiz, J.A.; Cano, H.; Marí, F. Intraspecies variability and conopeptide profiling of the injected venom of Conus ermineus. Peptides 2011, 32, 306–316, doi:10.1016/j.peptides.2010.11.014.
- Escoubas, P.; Quinton, L.; Nicholson, G.M. Venomics: Unravelling the complexity of animal venoms with mass spectrometry. J. Mass Spectrom. 2008, 43, 279–295, doi:10.1002/jms.1389.
- Prashanth, J.R.; Lewis, R.J.; Dutertre, S. Towards an integrated venomics approach for accelerated conopeptide discovery. Toxicon 2012, 60, 470–477, doi:10.1016/j.toxicon.2012.04.340.
- Samples Availability: Available from the authors.
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