Kocurin, the True Structure of PM181104, an Anti-Methicillin-Resistant Staphylococcus aureus (MRSA) Thiazolyl Peptide from the Marine-Derived Bacterium  Kocuria palustris

doi:10.3390/md11020387

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Mar. Drugs 2013, 11(2), 387-398; doi:10.3390/md11020387

Article

Kocurin, the True Structure of PM181104, an Anti-Methicillin-Resistant Staphylococcus aureus (MRSA) Thiazolyl Peptide from the Marine-Derived Bacterium Kocuria palustris

Jesús Martín¹, Thiciana da S. Sousa¹, Gloria Crespo¹, Sara Palomo¹, Ignacio González¹, José R. Tormo¹, Mercedes de la Cruz¹, Matthew Anderson^2,†, Russell T. Hill², Francisca Vicente¹, Olga Genilloud¹ and Fernando Reyes^1,*

¹ Fundación MEDINA, Centro de Excelencia en Investigación de Medicamentos Innovadores en Andalucía, Avda. del Conocimiento 3, Granada 18016, Spain ² Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, 701 E Pratt Street, Baltimore, MD 21202, USA ^† Current address: Algenol Biofuels, 16121 Lee Rd. Suite 110, Fort Myers, FL 33912, USA.

* Author to whom correspondence should be addressed.

Received: 11 December 2012; in revised form: 9 January 2013 / Accepted: 21 January 2013 / Published: 4 February 2013

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Abstract: A new thiazolyl peptide, kocurin (1), was isolated from culture broths of a marine-derived Kocuria palustris. Its structural elucidation was accomplished using a combination of spectroscopic and chemical methods, including HRMS, extensive 1D and 2D NMR analysis, MS/MS fragmentation, and chemical degradation and Marfey’s analysis of the resulting amino acid residues. The structure herein reported corrects that previously assigned to PM181104 (3). Kocurin displayed activity against methicillin-resistant Staphylococcus aureus (MRSA), with MIC values in the submicromolar range.

Keywords: marine-derived bacteria; Kocuria palustris; thiazolyl peptides; structure elucidation; antibacterial activity

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MDPI and ACS Style

Martín, J.; da S. Sousa, T.; Crespo, G.; Palomo, S.; González, I.; Tormo, J.R.; de la Cruz, M.; Anderson, M.; Hill, R.T.; Vicente, F.; Genilloud, O.; Reyes, F. Kocurin, the True Structure of PM181104, an Anti-Methicillin-Resistant Staphylococcus aureus (MRSA) Thiazolyl Peptide from the Marine-Derived Bacterium Kocuria palustris. Mar. Drugs 2013, 11, 387-398.

AMA Style

Martín J, da S. Sousa T, Crespo G, Palomo S, González I, Tormo JR, de la Cruz M, Anderson M, Hill RT, Vicente F, Genilloud O, Reyes F. Kocurin, the True Structure of PM181104, an Anti-Methicillin-Resistant Staphylococcus aureus (MRSA) Thiazolyl Peptide from the Marine-Derived Bacterium Kocuria palustris. Marine Drugs. 2013; 11(2):387-398.

Chicago/Turabian Style

Martín, Jesús; da S. Sousa, Thiciana; Crespo, Gloria; Palomo, Sara; González, Ignacio; Tormo, José R.; de la Cruz, Mercedes; Anderson, Matthew; Hill, Russell T.; Vicente, Francisca; Genilloud, Olga; Reyes, Fernando. 2013. "Kocurin, the True Structure of PM181104, an Anti-Methicillin-Resistant Staphylococcus aureus (MRSA) Thiazolyl Peptide from the Marine-Derived Bacterium Kocuria palustris." Mar. Drugs 11, no. 2: 387-398.

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