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Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China
Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 10 December 2012; in revised form: 6 January 2013 / Accepted: 9 January 2013 / Published: 31 January 2013
Abstract: Lipid A, the hydrophobic anchor of lipopolysaccharide, is an essential component in the outer membrane of most Gram-negative bacteria. Food-borne pathogen Cronobacter sakazakii synthesizes two lipid A species, differing by the length of the secondary acyl chain. In this work, we identified three genes ESA02293, ESA02951 and ESA01386 encoding for the late acyltransferases of lipid A biosynthesis pathway in C. sakazakii. Based on the sequence alignment, proteins YP_001438378.1 encoded by ESA02293, YP_001439016.1 encoded by ESA02951, and YP_001437482.1 encoded by ESA01386 are homologous to E. coli LpxL, LpxP and LpxM, respectively. Functions of the three acyltransferases were confirmed by overexpressing the genes in E. coli, isolating lipid As and analyzing their structures using an ESI/MS. C. sakazakii LpxL and LpxM transfer a C14:0 secondary acyl chain to the 2′- and 3′-position of lipid A, respectively. C. sakazakii LpxP can transfer either a C16:1 or a C14:0 secondary acyl chains to the 2′-position of lipid A.
Keywords: Cronobacter sakazakii; lipid A; acyltransferase; lpxL; lpxM; lpxP
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Cai, L.; Li, Y.; Tao, G.; Guo, W.; Zhang, C.; Wang, X. Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii. Mar. Drugs 2013, 11, 377-386.
Cai L, Li Y, Tao G, Guo W, Zhang C, Wang X. Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii. Marine Drugs. 2013; 11(2):377-386.
Cai, Liping; Li, Yanyan; Tao, Guanjun; Guo, Wen; Zhang, Chan; Wang, Xiaoyuan. 2013. "Identification of Three Genes Encoding for the Late Acyltransferases of Lipid A in Cronobacter sakazakii." Mar. Drugs 11, no. 2: 377-386.