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Mar. Drugs 2013, 11(11), 4419-4434; doi:10.3390/md11114419
Article

Crambescidin-816 Acts as a Fungicidal with More Potency than Crambescidin-800 and -830, Inducing Cell Cycle Arrest, Increased Cell Size and Apoptosis in Saccharomyces cerevisiae

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1 Department of Pharmacology, Faculty of Veterinary, University of Santiago de Compostela (USC), Campus Lugo, Lugo 27002, Spain 2 Department of Physiology, Faculty of Veterinary, University of Santiago de Compostela (USC), Campus Lugo, Lugo 27002, Spain 3 Nice Institute of Chemistry-PCRE, UMR 7272 CNRS, Faculty of Sciences, University of Nice Sophia Antipolis, Parc Valrose 06108 Nice, France
* Author to whom correspondence should be addressed.
Received: 29 August 2013 / Revised: 25 October 2013 / Accepted: 29 October 2013 / Published: 8 November 2013
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Abstract

In this paper, we show the effect of crambescidin-816, -800, and -830 on Saccharomyces cerevisiae viability. We determined that, of the three molecules tested, crambescidin-816 was the most potent. Based on this result, we continued by determining the effect of crambescidin-816 on the cell cycle of this yeast. The compound induced cell cycle arrest in G2/M followed by an increase in cell DNA content and size. When the type of cell death was analyzed, we observed that crambescidin-816 induced apoptosis. The antifungal effect indicates that crambescidins, and mostly crambescidin-816, could serve as a lead compound to fight fungal infections.
Keywords: crambescidine-816; antifungal; cell cycle arrest; apoptosis crambescidine-816; antifungal; cell cycle arrest; apoptosis
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Rubiolo, J.A.; Ternon, E.; López-Alonso, H.; Thomas, O.P.; Vega, F.V.; Vieytes, M.R.; Botana, L.M. Crambescidin-816 Acts as a Fungicidal with More Potency than Crambescidin-800 and -830, Inducing Cell Cycle Arrest, Increased Cell Size and Apoptosis in Saccharomyces cerevisiae. Mar. Drugs 2013, 11, 4419-4434.

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