Abstract: CMP-Neu5Ac is an important sugar nucleotide for biosynthesis of sialic acid and its conjugates. In this paper, a large-scale production system of CMP-Neu5Ac by a single strain is reported. The co-expression of Neu5Ac aldolase (EC18.104.22.168) and CMP-Neu5Ac synthetase (EC 22.214.171.124) was achieved by constructing individual genes into one plasmid and having a single culture that has both NeuAc aldolase and CMP-Neu5Ac synthetase activities. Overall this system only employed N-acetylmannosamine, excess of pyruvate and CTP to produce CMP-Neu5Ac. This work has demonstrated that a large-scale synthesis of sialic acid-derived oligosaccharides could be achieved economically and efficiently through a single, biosynthetic pathway engineered microorganism.
Keywords: biosynthesis; cytidine 5’-monophospho-N-acetylneuraminic acid; NeuAc aldolase; CMP-NeuAc synthetase
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Song, J.; Zhang, H.; Wu, B.; Zhang, Y.; Li, H.; Xiao, M.; Wang, P.G. Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac. Mar. Drugs 2003, 1, 34-45.
Song J, Zhang H, Wu B, Zhang Y, Li H, Xiao M, Wang PG. Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac. Marine Drugs. 2003; 1(1):34-45.
Song, Jing; Zhang, Hesheng; Wu, Bingyuan; Zhang, Yingxin; Li, Hanfen; Xiao, Min; Wang, Peng G. 2003. "Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac." Mar. Drugs 1, no. 1: 34-45.