Abstract: CMP-Neu5Ac is an important sugar nucleotide for biosynthesis of sialic acid and its conjugates. In this paper, a large-scale production system of CMP-Neu5Ac by a single strain is reported. The co-expression of Neu5Ac aldolase (EC184.108.40.206) and CMP-Neu5Ac synthetase (EC 220.127.116.11) was achieved by constructing individual genes into one plasmid and having a single culture that has both NeuAc aldolase and CMP-Neu5Ac synthetase activities. Overall this system only employed N-acetylmannosamine, excess of pyruvate and CTP to produce CMP-Neu5Ac. This work has demonstrated that a large-scale synthesis of sialic acid-derived oligosaccharides could be achieved economically and efficiently through a single, biosynthetic pathway engineered microorganism.
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Song, J.; Zhang, H.; Wu, B.; Zhang, Y.; Li, H.; Xiao, M.; Wang, P.G. Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac. Mar. Drugs 2003, 1, 34-45.
Song J, Zhang H, Wu B, Zhang Y, Li H, Xiao M, Wang PG. Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac. Marine Drugs. 2003; 1(1):34-45.
Song, Jing; Zhang, Hesheng; Wu, Bingyuan; Zhang, Yingxin; Li, Hanfen; Xiao, Min; Wang, Peng G. 2003. "Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac." Mar. Drugs 1, no. 1: 34-45.