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Mar. Drugs 2003, 1(1), 34-45; doi:10.3390/md101034
Article

Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac

1, 2, 2, 2, 2, 1 and 1,2,*
1 State Key Laboratory of Microbial Technology, College of Life Sciences, Shandong University, Jinan 250100, Shandong Province, P. R. China 2 Department of Biochemistry, College of Biological Sciences, The Ohio State University, Columbus, Ohio 43210, USA
* Author to whom correspondence should be addressed.
Received: 10 October 2003 / Accepted: 13 November 2003 / Published: 26 November 2003
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Abstract

CMP-Neu5Ac is an important sugar nucleotide for biosynthesis of sialic acid and its conjugates. In this paper, a large-scale production system of CMP-Neu5Ac by a single strain is reported. The co-expression of Neu5Ac aldolase (EC4.1.3.3) and CMP-Neu5Ac synthetase (EC 2.7.7.43) was achieved by constructing individual genes into one plasmid and having a single culture that has both NeuAc aldolase and CMP-Neu5Ac synthetase activities. Overall this system only employed N-acetylmannosamine, excess of pyruvate and CTP to produce CMP-Neu5Ac. This work has demonstrated that a large-scale synthesis of sialic acid-derived oligosaccharides could be achieved economically and efficiently through a single, biosynthetic pathway engineered microorganism.
Keywords: biosynthesis; cytidine 5’-monophospho-N-acetylneuraminic acid; NeuAc aldolase; CMP-NeuAc synthetase biosynthesis; cytidine 5’-monophospho-N-acetylneuraminic acid; NeuAc aldolase; CMP-NeuAc synthetase
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Song, J.; Zhang, H.; Wu, B.; Zhang, Y.; Li, H.; Xiao, M.; Wang, P.G. Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac. Mar. Drugs 2003, 1, 34-45.

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