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Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac
State Key Laboratory of Microbial Technology, College of Life Sciences, Shandong University, Jinan 250100, Shandong Province, P. R. China
Department of Biochemistry, College of Biological Sciences, The Ohio State University, Columbus, Ohio 43210, USA
* Author to whom correspondence should be addressed.
Received: 10 October 2003; Accepted: 13 November 2003 / Published: 26 November 2003
Abstract: CMP-Neu5Ac is an important sugar nucleotide for biosynthesis of sialic acid and its conjugates. In this paper, a large-scale production system of CMP-Neu5Ac by a single strain is reported. The co-expression of Neu5Ac aldolase (EC18.104.22.168) and CMP-Neu5Ac synthetase (EC 22.214.171.124) was achieved by constructing individual genes into one plasmid and having a single culture that has both NeuAc aldolase and CMP-Neu5Ac synthetase activities. Overall this system only employed N-acetylmannosamine, excess of pyruvate and CTP to produce CMP-Neu5Ac. This work has demonstrated that a large-scale synthesis of sialic acid-derived oligosaccharides could be achieved economically and efficiently through a single, biosynthetic pathway engineered microorganism.
Keywords: biosynthesis; cytidine 5’-monophospho-N-acetylneuraminic acid; NeuAc aldolase; CMP-NeuAc synthetase
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MDPI and ACS Style
Song, J.; Zhang, H.; Wu, B.; Zhang, Y.; Li, H.; Xiao, M.; Wang, P.G. Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac. Mar. Drugs 2003, 1, 34-45.
Song J, Zhang H, Wu B, Zhang Y, Li H, Xiao M, Wang PG. Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac. Marine Drugs. 2003; 1(1):34-45.
Song, Jing; Zhang, Hesheng; Wu, Bingyuan; Zhang, Yingxin; Li, Hanfen; Xiao, Min; Wang, Peng G. 2003. "Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac." Mar. Drugs 1, no. 1: 34-45.