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Mar. Drugs 2003, 1(1), 34-45; doi:10.3390/md101034
Article

Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac

1, 2, 2, 2, 2, 1 and 1,2,*
Received: 10 October 2003; Accepted: 13 November 2003 / Published: 26 November 2003
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Abstract: CMP-Neu5Ac is an important sugar nucleotide for biosynthesis of sialic acid and its conjugates. In this paper, a large-scale production system of CMP-Neu5Ac by a single strain is reported. The co-expression of Neu5Ac aldolase (EC4.1.3.3) and CMP-Neu5Ac synthetase (EC 2.7.7.43) was achieved by constructing individual genes into one plasmid and having a single culture that has both NeuAc aldolase and CMP-Neu5Ac synthetase activities. Overall this system only employed N-acetylmannosamine, excess of pyruvate and CTP to produce CMP-Neu5Ac. This work has demonstrated that a large-scale synthesis of sialic acid-derived oligosaccharides could be achieved economically and efficiently through a single, biosynthetic pathway engineered microorganism.
Keywords: biosynthesis; cytidine 5’-monophospho-N-acetylneuraminic acid; NeuAc aldolase; CMP-NeuAc synthetase biosynthesis; cytidine 5’-monophospho-N-acetylneuraminic acid; NeuAc aldolase; CMP-NeuAc synthetase
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Song, J.; Zhang, H.; Wu, B.; Zhang, Y.; Li, H.; Xiao, M.; Wang, P.G. Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac. Mar. Drugs 2003, 1, 34-45.

AMA Style

Song J, Zhang H, Wu B, Zhang Y, Li H, Xiao M, Wang PG. Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac. Marine Drugs. 2003; 1(1):34-45.

Chicago/Turabian Style

Song, Jing; Zhang, Hesheng; Wu, Bingyuan; Zhang, Yingxin; Li, Hanfen; Xiao, Min; Wang, Peng G. 2003. "Reassembled Biosynthetic Pathway for a Large-scale Synthesis of CMP-Neu5Ac." Mar. Drugs 1, no. 1: 34-45.


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