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Pharmaceuticals 2016, 9(4), 60; doi:10.3390/ph9040060

pMPES: A Modular Peptide Expression System for the Delivery of Antimicrobial Peptides to the Site of Gastrointestinal Infections Using Probiotics

1
Department of Chemical Engineering and Materials Science, University of Minnesota, 421 Washington Ave SE, Minneapolis, MN 55455, USA
2
Breck School, 123 Ottawa Ave N, Golden Valley, MN 55422, USA
*
Author to whom correspondence should be addressed.
Received: 6 July 2016 / Accepted: 14 September 2016 / Published: 5 October 2016
(This article belongs to the Special Issue Antimicrobial Peptides: Expanded Activity Spectrum and Applications)
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Abstract

Antimicrobial peptides are a promising alternative to traditional antibiotics, but their utility is limited by high production costs and poor bioavailability profiles. Bacterial production and delivery of antimicrobial peptides (AMPs) directly at the site of infection may offer a path for effective therapeutic application. In this study, we have developed a vector that can be used for the production and secretion of seven antimicrobial peptides from both Escherichia coli MC1061 F’ and probiotic E.coli Nissle 1917. The vector pMPES (Modular Peptide Expression System) employs the Microcin V (MccV) secretion system and a powerful synthetic promoter to drive AMP production. Herein, we demonstrate the capacity of pMPES to produce inhibitory levels of MccV, Microcin L (MccL), Microcin N (McnN), Enterocin A (EntA), Enterocin P (EntP), Hiracin JM79 (HirJM79) and Enterocin B (EntB). To our knowledge, this is the first demonstration of such a broadly-applicable secretion system for AMP production. This type of modular expression system could expedite the development of sorely needed antimicrobial technologies View Full-Text
Keywords: antimicrobialpeptides;microcinV;heterologousproduction;secretion;E.coliNissle1917; antimicrobial probiotics antimicrobialpeptides;microcinV;heterologousproduction;secretion;E.coliNissle1917; antimicrobial probiotics
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MDPI and ACS Style

Geldart, K.; Forkus, B.; McChesney, E.; McCue, M.; Kaznessis, Y.N. pMPES: A Modular Peptide Expression System for the Delivery of Antimicrobial Peptides to the Site of Gastrointestinal Infections Using Probiotics. Pharmaceuticals 2016, 9, 60.

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