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Pharmaceuticals 2016, 9(3), 51; doi:10.3390/ph9030051

Design and Prototype of an Automated Column-Switching HPLC System for Radiometabolite Analysis

1
Division of Nuclear Medicine and Molecular Imaging & Gordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
2
Advion Inc., Research and Development, Ithaca, NY 14850, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Michel Monclus
Received: 25 July 2016 / Revised: 3 August 2016 / Accepted: 10 August 2016 / Published: 17 August 2016
(This article belongs to the Special Issue New Challenges in Radiochemistry)
View Full-Text   |   Download PDF [1133 KB, uploaded 17 August 2016]   |  

Abstract

Column-switching high performance liquid chromatography (HPLC) is extensively used for the critical analysis of radiolabeled ligands and their metabolites in plasma. However, the lack of streamlined apparatus and consequently varying protocols remain as a challenge among positron emission tomography laboratories. We report here the prototype apparatus and implementation of a fully automated and simplified column-switching procedure to allow for the easy and automated determination of radioligands and their metabolites in up to 5 mL of plasma. The system has been used with conventional UV and coincidence radiation detectors, as well as with a single quadrupole mass spectrometer. View Full-Text
Keywords: positron emission tomography; radiometabolite; column-switching HPLC; plasma positron emission tomography; radiometabolite; column-switching HPLC; plasma
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Vasdev, N.; Collier, T.L. Design and Prototype of an Automated Column-Switching HPLC System for Radiometabolite Analysis. Pharmaceuticals 2016, 9, 51.

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