Pharmaceuticals 2012, 5(1), 49-60; doi:10.3390/ph5010049

Anti-HCV RNA Aptamers Targeting the Genomic cis-Acting Replication Element

Instituto de Parasitología y Biomedicina “López-Neyra”, IPBLN-CSIC, P.T. Ciencias de la Salud, Av. del Conocimiento s/n, Armilla, 18100 Granada, Spain
* Author to whom correspondence should be addressed.
Received: 28 November 2011; in revised form: 15 December 2011 / Accepted: 22 December 2011 / Published: 28 December 2011
(This article belongs to the Special Issue Aptamer-Based Therapeutics)
PDF Full-text Download PDF Full-Text [456 KB, uploaded 28 December 2011 11:47 CET]
Abstract: Hepatitis C virus (HCV) replication is dependent on the existence of several highly conserved functional genomic RNA domains. The cis-acting replication element (CRE), located within the 3' end of the NS5B coding region of the HCV genome, has been shown essential for efficient viral replication. Its sequence and structural features determine its involvement in functional interactions with viral RNA-dependent RNA polymerase and distant RNA domains of the viral genome. This work reports the use of an in vitro selection strategy to select aptamer RNA molecules against the complete HCV-CRE. After six selection cycles, five potential target sites were identified within this domain. Inhibition assays using a sample of representative aptamers showed that the selected RNAs significantly inhibit the replication (>80%) of a subgenomic HCV replicon in Huh-7 cell cultures. These results highlight the potential of aptamer RNA molecules as therapeutic antiviral agents.
Keywords: aptamer selection; SELEX; RNA aptamers; anti-HCV aptamers; aptamer therapeutics

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Marton, S.; Berzal-Herranz, B.; Garmendia, E.; Cueto, F.J.; Berzal-Herranz, A. Anti-HCV RNA Aptamers Targeting the Genomic cis-Acting Replication Element. Pharmaceuticals 2012, 5, 49-60.

AMA Style

Marton S, Berzal-Herranz B, Garmendia E, Cueto FJ, Berzal-Herranz A. Anti-HCV RNA Aptamers Targeting the Genomic cis-Acting Replication Element. Pharmaceuticals. 2012; 5(1):49-60.

Chicago/Turabian Style

Marton, Soledad; Berzal-Herranz, Beatriz; Garmendia, Eva; Cueto, Francisco J.; Berzal-Herranz, Alfredo. 2012. "Anti-HCV RNA Aptamers Targeting the Genomic cis-Acting Replication Element." Pharmaceuticals 5, no. 1: 49-60.

Pharmaceuticals EISSN 1424-8247 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert