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Pharmaceuticals 2017, 10(3), 66; doi:10.3390/ph10030066

Modernization of Enoxaparin Molecular Weight Determination Using Homogeneous Standards

1
Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC 27599, USA
2
U.S. Pharmacopeia, Rockville, MD 20852, USA
3
Center for Scientific Review, National Institutes of Health, Bethesda, MD 20892, USA
*
Author to whom correspondence should be addressed.
Received: 1 May 2017 / Revised: 6 July 2017 / Accepted: 19 July 2017 / Published: 22 July 2017
(This article belongs to the Special Issue Glycosaminoglycans and Proteoglycans)
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Abstract

Enoxaparin is a low-molecular weight heparin used to treat thrombotic disorders. Following the fatal contamination of the heparin supply chain in 2007–2008, the U.S. Pharmacopeia (USP) and U.S. Food and Drug Administration (FDA) have worked extensively to modernize the unfractionated heparin and enoxaparin monographs. As a result, the determination of molecular weight (MW) has been added to the monograph as a measure to strengthen the quality testing and to increase the protection of the global supply of this life-saving drug. The current USP calibrant materials used for enoxaparin MW determination are composed of a mixture of oligosaccharides; however, they are difficult to reproduce as the calibrants have ill-defined structures due to the heterogeneity of the heparin parent material. To address this issue, we describe a promising approach consisting of a predictive computational model built from a library of chemoenzymatically synthesized heparin oligosaccharides for enoxaparin MW determination. Here, we demonstrate that this test can be performed with greater efficiency by coupling synthetic oligosaccharides with the power of computational modeling. Our approach is expected to improve the MW measurement for enoxaparin. View Full-Text
Keywords: enoxaparin; USP; MW determination; oligosaccharide calibrants; computational modeling; HPLC; compendial test enoxaparin; USP; MW determination; oligosaccharide calibrants; computational modeling; HPLC; compendial test
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MDPI and ACS Style

Arnold, K.M.; Capuzzi, S.J.; Xu, Y.; Muratov, E.N.; Carrick, K.; Szajek, A.Y.; Tropsha, A.; Liu, J. Modernization of Enoxaparin Molecular Weight Determination Using Homogeneous Standards. Pharmaceuticals 2017, 10, 66.

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