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Sensors 2009, 9(8), 6084-6100; doi:10.3390/s90806084
Article

From Lateral Flow Devices to a Novel Nano-Color Microfluidic Assay

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Received: 15 July 2009; in revised form: 24 July 2009 / Accepted: 29 July 2009 / Published: 31 July 2009
(This article belongs to the Special Issue Nanotechnological Advances in Biosensors)
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Abstract: Improving the performance of traditional diagnostic lateral flow assays combined with new manufacturing technologies is a primary goal in the research and development plans of diagnostic companies. Taking into consideration the components of lateral flow diagnostic test kits; innovation can include modification of labels, materials and device design. In recent years, Resonance-Enhanced Absorption (REA) of metal nano-particles has shown excellent applicability in bio-sensing for the detection of a variety of bio-molecular binding interactions. In a novel approach, we have now integrated REA-assays in a diagnostic microfluidic setup thus resolving the bottleneck of long incubation times inherent in previously existing REA-assays and simultaneously integrated automated fabrication techniques for diagnostics manufacture. Due to the roller-coating based technology and chemical resistance, we used PET-co-polyester as a substrate and a CO2 laser ablation system as a fast, highly precise and contactless alternative to classical micro-milling. It was possible to detect biological binding within three minutes – visible to the eye as colored text readout within the REA-fluidic device. A two-minute in-situ silver enhancement was able to enhance the resonant color additionally, if required.
Keywords: lateral flow assays (LFAs); resonance-enhanced absorption (REA); AuNPs-bioconjugate; polyethylene terephthalate (PET); CO2 laser ablation lateral flow assays (LFAs); resonance-enhanced absorption (REA); AuNPs-bioconjugate; polyethylene terephthalate (PET); CO2 laser ablation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Assadollahi, S.; Reininger, C.; Palkovits, R.; Pointl, P.; Schalkhammer, T. From Lateral Flow Devices to a Novel Nano-Color Microfluidic Assay. Sensors 2009, 9, 6084-6100.

AMA Style

Assadollahi S, Reininger C, Palkovits R, Pointl P, Schalkhammer T. From Lateral Flow Devices to a Novel Nano-Color Microfluidic Assay. Sensors. 2009; 9(8):6084-6100.

Chicago/Turabian Style

Assadollahi, Saied; Reininger, Christiane; Palkovits, Roland; Pointl, Peter; Schalkhammer, Thomas. 2009. "From Lateral Flow Devices to a Novel Nano-Color Microfluidic Assay." Sensors 9, no. 8: 6084-6100.



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