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Sensors 2009, 9(8), 6084-6100; doi:10.3390/s90806084

From Lateral Flow Devices to a Novel Nano-Color Microfluidic Assay

1 Attophotonics Biosciences GmbH, Viktor Kaplan Strasse 2, A-2700 Wiener Neustadt, Austria 2 Max F. Perutz Laboratories, Department of Biochemistry and Molecular Cell Biology, University of Vienna, A-1030 Vienna, Austria 3 Departments of Micro and Nanosystems, University of Applied Sciences, A-2700 Wiener Neustadt, Austria
* Author to whom correspondence should be addressed.
Received: 15 July 2009 / Revised: 24 July 2009 / Accepted: 29 July 2009 / Published: 31 July 2009
(This article belongs to the Special Issue Nanotechnological Advances in Biosensors)
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Improving the performance of traditional diagnostic lateral flow assays combined with new manufacturing technologies is a primary goal in the research and development plans of diagnostic companies. Taking into consideration the components of lateral flow diagnostic test kits; innovation can include modification of labels, materials and device design. In recent years, Resonance-Enhanced Absorption (REA) of metal nano-particles has shown excellent applicability in bio-sensing for the detection of a variety of bio-molecular binding interactions. In a novel approach, we have now integrated REA-assays in a diagnostic microfluidic setup thus resolving the bottleneck of long incubation times inherent in previously existing REA-assays and simultaneously integrated automated fabrication techniques for diagnostics manufacture. Due to the roller-coating based technology and chemical resistance, we used PET-co-polyester as a substrate and a CO2 laser ablation system as a fast, highly precise and contactless alternative to classical micro-milling. It was possible to detect biological binding within three minutes – visible to the eye as colored text readout within the REA-fluidic device. A two-minute in-situ silver enhancement was able to enhance the resonant color additionally, if required.
Keywords: lateral flow assays (LFAs); resonance-enhanced absorption (REA); AuNPs-bioconjugate; polyethylene terephthalate (PET); CO2 laser ablation lateral flow assays (LFAs); resonance-enhanced absorption (REA); AuNPs-bioconjugate; polyethylene terephthalate (PET); CO2 laser ablation
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Assadollahi, S.; Reininger, C.; Palkovits, R.; Pointl, P.; Schalkhammer, T. From Lateral Flow Devices to a Novel Nano-Color Microfluidic Assay. Sensors 2009, 9, 6084-6100.

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