Next Article in Journal
Building Reconstruction by Target Based Graph Matching on Incomplete Laser Data: Analysis and Limitations
Next Article in Special Issue
A Novel Design of Grooved Fibers for Fiber-Optic Localized Plasmon Resonance Biosensors
Previous Article in Journal
Meat Quality Assessment by Electronic Nose (Machine Olfaction Technology)
Previous Article in Special Issue
Optical Biosensors Based on Semiconductor Nanostructures
Sensors 2009, 9(8), 6084-6100; doi:10.3390/s90806084

From Lateral Flow Devices to a Novel Nano-Color Microfluidic Assay

1 Attophotonics Biosciences GmbH, Viktor Kaplan Strasse 2, A-2700 Wiener Neustadt, Austria 2 Max F. Perutz Laboratories, Department of Biochemistry and Molecular Cell Biology, University of Vienna, A-1030 Vienna, Austria 3 Departments of Micro and Nanosystems, University of Applied Sciences, A-2700 Wiener Neustadt, Austria
* Author to whom correspondence should be addressed.
Received: 15 July 2009 / Revised: 24 July 2009 / Accepted: 29 July 2009 / Published: 31 July 2009
(This article belongs to the Special Issue Nanotechnological Advances in Biosensors)
View Full-Text   |   Download PDF [680 KB, uploaded 21 June 2014]   |   Browse Figures


Improving the performance of traditional diagnostic lateral flow assays combined with new manufacturing technologies is a primary goal in the research and development plans of diagnostic companies. Taking into consideration the components of lateral flow diagnostic test kits; innovation can include modification of labels, materials and device design. In recent years, Resonance-Enhanced Absorption (REA) of metal nano-particles has shown excellent applicability in bio-sensing for the detection of a variety of bio-molecular binding interactions. In a novel approach, we have now integrated REA-assays in a diagnostic microfluidic setup thus resolving the bottleneck of long incubation times inherent in previously existing REA-assays and simultaneously integrated automated fabrication techniques for diagnostics manufacture. Due to the roller-coating based technology and chemical resistance, we used PET-co-polyester as a substrate and a CO2 laser ablation system as a fast, highly precise and contactless alternative to classical micro-milling. It was possible to detect biological binding within three minutes – visible to the eye as colored text readout within the REA-fluidic device. A two-minute in-situ silver enhancement was able to enhance the resonant color additionally, if required.
Keywords: lateral flow assays (LFAs); resonance-enhanced absorption (REA); AuNPs-bioconjugate; polyethylene terephthalate (PET); CO2 laser ablation lateral flow assays (LFAs); resonance-enhanced absorption (REA); AuNPs-bioconjugate; polyethylene terephthalate (PET); CO2 laser ablation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Assadollahi, S.; Reininger, C.; Palkovits, R.; Pointl, P.; Schalkhammer, T. From Lateral Flow Devices to a Novel Nano-Color Microfluidic Assay. Sensors 2009, 9, 6084-6100.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert