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Sensors 2009, 9(6), 4635-4648; doi:10.3390/s90604635

Immobilization of HRP in Mesoporous Silica and Its Application for the Construction of Polyaniline Modified Hydrogen Peroxide Biosensor

Department of Chemical and Materials Engineering, Tunghai University, Taichung 40704, Taiwan
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Received: 21 April 2009 / Revised: 3 June 2009 / Accepted: 5 June 2009 / Published: 12 June 2009
(This article belongs to the Section Biosensors)
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Abstract

Polyaniline (PANI), an attractive conductive polymer, has been successfully applied in fabricating various types of enzyme-based biosensors. In this study, we have employed mesoporous silica SBA-15 to stably entrap horseradish peroxidase (HRP), and then deposited the loaded SBA-15 on the PANI modified platinum electrode to construct a GA/SBA-15(HRP)/PANI/Pt biosensor. The mesoporous structures and morphologies of SBA-15 with or without HRP were characterized. Enzymatic protein assays were employed to evaluate HRP immobilization efficiency. Our results demonstrated that the constructed biosensor displayed a fine linear correlation between cathodic response and H2O2 concentration in the range of 0.02 to 18.5 mM, with enhanced sensitivity. In particular, the current approach provided the PANI modified biosensor with improved stability for multiple measurements. View Full-Text
Keywords: polyaniline; SBA-15; horseradish peroxidase; hydrogen peroxide; biosensor polyaniline; SBA-15; horseradish peroxidase; hydrogen peroxide; biosensor
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Chen, C.-C.; Do, J.-S.; Gu, Y. Immobilization of HRP in Mesoporous Silica and Its Application for the Construction of Polyaniline Modified Hydrogen Peroxide Biosensor. Sensors 2009, 9, 4635-4648.

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