Next Article in Journal
Metric Potential of a 3D Measurement System Based on Digital Compact Cameras
Previous Article in Journal
Global Oxygen Detection in Water Using Luminescent Probe on Anodized Aluminum
Article Menu

Export Article

Open AccessArticle
Sensors 2009, 9(6), 4164-4177; doi:10.3390/s90604164

Fluorescence Detection and Discrimination of ss- and ds-DNA with a Water Soluble Oligopyrene Derivative

Key Lab of Bio-organic Phosphorous Chemistry and Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China
*
Author to whom correspondence should be addressed.
Received: 9 April 2009 / Revised: 1 May 2009 / Accepted: 21 May 2009 / Published: 2 June 2009
(This article belongs to the Section Biosensors)
View Full-Text   |   Download PDF [416 KB, uploaded 21 June 2014]   |  

Abstract

A novel water-soluble cationic conjugated oligopyrene derivative, oligo(N1,N1,N1,N4,N4,N4-hexamethyl-2-(4-(pyren-1-yl) butane-1,4-diaminium bromide) (OHPBDB), was synthesized by a combination of chemical and electrochemical synthesis techniques. Each oligomer chain has five pyrene derivative repeating units and brings 10 positive charges. OHPBDB showed high and rapid fluorescence quenching in aqueous media upon addition of trace amounts of single-stranded (ss) and double-stranded (ds) DNA. The Stern-Volmer constants for ss- and ds-DNA were measured to be as high as 1.3 × 108 mol-1·L and 1.2 × 108 mol-1·L, respectively. On the other hand, distinct fluorescence enhancement of OHPBDB upon addition of large amount of ss-DNA or ds-DNA was observed. Furthermore, ss-DNA showed much stronger fluorescence enhancement than that of ds-DNA, thus yielding a clear and simple signal useful for the discrimination between ss- and ds-DNA in aqueous media. View Full-Text
Keywords: water-soluble oligopyrene derivative; DNA; fluorescence quenching water-soluble oligopyrene derivative; DNA; fluorescence quenching
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Chen, Y.; Shi, G. Fluorescence Detection and Discrimination of ss- and ds-DNA with a Water Soluble Oligopyrene Derivative. Sensors 2009, 9, 4164-4177.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top