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On-Chip Immunoassay for Determination of Urinary Albumin
Centre for Excellence in Omics-Nano Medical Technology Project Development, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand
Graduate Programme in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand
Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Centre, Pathum Thani, 12120, Thailand
* Author to whom correspondence should be addressed.
Received: 11 October 2009; in revised form: 13 November 2009 / Accepted: 30 November 2009 / Published: 10 December 2009
Abstract: An immunoassay performed on a portable microfluidic device was evaluated for the determination of urinary albumin. An increase in absorbance at 500 nm resulting from immunoagglutination was monitored directly on the poly(dimethylsiloxane) (PDMS) microchip using a portable miniature fibre-optic spectrometer. A calibration curve was linear up to 10 mg L–1 (r2 = 0.993), with a detection limit of 0.81 mg L–1 (S/N = 3). The proposed system showed good precision, with relative standard deviations (RSDs) of 5.1%, when evaluated with 10 mg L–1 albumin (n = 10). Determination of urinary albumin with the proposed system gave results highly similar to those determined by the conventional spectrophotometric method using immunoturbidimetric detection (r2 = 0.995; n = 15).
Keywords: microalbuminuria; microfluidic system; on-chip immunoassay; poly(dimethylsiloxane)
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Laiwattanapaisal, W.; Songjaroen, T.; Maturos, T.; Lomas, T.; Sappat, A.; Tuantranont, A. On-Chip Immunoassay for Determination of Urinary Albumin. Sensors 2009, 9, 10066-10079.
Laiwattanapaisal W, Songjaroen T, Maturos T, Lomas T, Sappat A, Tuantranont A. On-Chip Immunoassay for Determination of Urinary Albumin. Sensors. 2009; 9(12):10066-10079.
Laiwattanapaisal, Wanida; Songjaroen, Temsiri; Maturos, Thitima; Lomas, Tanom; Sappat, Assawapong; Tuantranont, Adisorn. 2009. "On-Chip Immunoassay for Determination of Urinary Albumin." Sensors 9, no. 12: 10066-10079.