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• Laiwattanapaisal, W
• Songjaroen, T
• Maturos, T
• Lomas, T
• Sappat, A
• Tuantranont, A
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• Laiwattanapaisal, W
• Songjaroen, T
• Maturos, T
• Lomas, T
• Sappat, A
• Tuantranont, A
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• Laiwattanapaisal, W
• Songjaroen, T
• Maturos, T
• Lomas, T
• Sappat, A
• Tuantranont, A

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Sensors 2009, 9(12), 10066-10079; doi:10.3390/s91210066

Article

On-Chip Immunoassay for Determination of Urinary Albumin

Wanida Laiwattanapaisal^1,* , Temsiri Songjaroen² , Thitima Maturos³ , Tanom Lomas³ , Assawapong Sappat³  and Adisorn Tuantranont³

¹ Centre for Excellence in Omics-Nano Medical Technology Project Development, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand ² Graduate Programme in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand ³ Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Centre, Pathum Thani, 12120, Thailand

* Author to whom correspondence should be addressed.

Received: 11 October 2009; in revised form: 13 November 2009 / Accepted: 30 November 2009 / Published: 10 December 2009

(This article belongs to the Section Chemical Sensors)

Download PDF Full-Text [544 KB, uploaded 23 December 2009 16:11 CET]

Abstract: An immunoassay performed on a portable microfluidic device was evaluated for the determination of urinary albumin. An increase in absorbance at 500 nm resulting from immunoagglutination was monitored directly on the poly(dimethylsiloxane) (PDMS) microchip using a portable miniature fibre-optic spectrometer. A calibration curve was linear up to 10 mg L^–1 (r² = 0.993), with a detection limit of 0.81 mg L^–1 (S/N = 3). The proposed system showed good precision, with relative standard deviations (RSDs) of 5.1%, when evaluated with 10 mg L^–1 albumin (n = 10). Determination of urinary albumin with the proposed system gave results highly similar to those determined by the conventional spectrophotometric method using immunoturbidimetric detection (r² = 0.995; n = 15).

Keywords: microalbuminuria; microfluidic system; on-chip immunoassay; poly(dimethylsiloxane)

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