Abstract: An immunoassay performed on a portable microfluidic device was evaluated for the determination of urinary albumin. An increase in absorbance at 500 nm resulting from immunoagglutination was monitored directly on the poly(dimethylsiloxane) (PDMS) microchip using a portable miniature fibre-optic spectrometer. A calibration curve was linear up to 10 mg L–1 (r2 = 0.993), with a detection limit of 0.81 mg L–1 (S/N = 3). The proposed system showed good precision, with relative standard deviations (RSDs) of 5.1%, when evaluated with 10 mg L–1 albumin (n = 10). Determination of urinary albumin with the proposed system gave results highly similar to those determined by the conventional spectrophotometric method using immunoturbidimetric detection (r2 = 0.995; n = 15).
Keywords: microalbuminuria; microfluidic system; on-chip immunoassay; poly(dimethylsiloxane)
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Laiwattanapaisal, W.; Songjaroen, T.; Maturos, T.; Lomas, T.; Sappat, A.; Tuantranont, A. On-Chip Immunoassay for Determination of Urinary Albumin. Sensors 2009, 9, 10066-10079.
Laiwattanapaisal W, Songjaroen T, Maturos T, Lomas T, Sappat A, Tuantranont A. On-Chip Immunoassay for Determination of Urinary Albumin. Sensors. 2009; 9(12):10066-10079.
Laiwattanapaisal, Wanida; Songjaroen, Temsiri; Maturos, Thitima; Lomas, Tanom; Sappat, Assawapong; Tuantranont, Adisorn. 2009. "On-Chip Immunoassay for Determination of Urinary Albumin." Sensors 9, no. 12: 10066-10079.