Sensors 2009, 9(12), 10066-10079; doi:10.3390/s91210066

On-Chip Immunoassay for Determination of Urinary Albumin

1,* email, 2email, 3email, 3email, 3email and 3email
Received: 11 October 2009; in revised form: 13 November 2009 / Accepted: 30 November 2009 / Published: 10 December 2009
(This article belongs to the Section Physical Sensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: An immunoassay performed on a portable microfluidic device was evaluated for the determination of urinary albumin. An increase in absorbance at 500 nm resulting from immunoagglutination was monitored directly on the poly(dimethylsiloxane) (PDMS) microchip using a portable miniature fibre-optic spectrometer. A calibration curve was linear up to 10 mg L–1 (r2 = 0.993), with a detection limit of 0.81 mg L–1 (S/N = 3). The proposed system showed good precision, with relative standard deviations (RSDs) of 5.1%, when evaluated with 10 mg L–1 albumin (n = 10). Determination of urinary albumin with the proposed system gave results highly similar to those determined by the conventional spectrophotometric method using immunoturbidimetric detection (r2 = 0.995; n = 15).
Keywords: microalbuminuria; microfluidic system; on-chip immunoassay; poly(dimethylsiloxane)
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MDPI and ACS Style

Laiwattanapaisal, W.; Songjaroen, T.; Maturos, T.; Lomas, T.; Sappat, A.; Tuantranont, A. On-Chip Immunoassay for Determination of Urinary Albumin. Sensors 2009, 9, 10066-10079.

AMA Style

Laiwattanapaisal W, Songjaroen T, Maturos T, Lomas T, Sappat A, Tuantranont A. On-Chip Immunoassay for Determination of Urinary Albumin. Sensors. 2009; 9(12):10066-10079.

Chicago/Turabian Style

Laiwattanapaisal, Wanida; Songjaroen, Temsiri; Maturos, Thitima; Lomas, Tanom; Sappat, Assawapong; Tuantranont, Adisorn. 2009. "On-Chip Immunoassay for Determination of Urinary Albumin." Sensors 9, no. 12: 10066-10079.

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