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Sensors 2009, 9(12), 10066-10079; doi:10.3390/s91210066
Article

On-Chip Immunoassay for Determination of Urinary Albumin

1,* , 2
,
3
,
3
,
3
 and
3
1 Centre for Excellence in Omics-Nano Medical Technology Project Development, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand 2 Graduate Programme in Clinical Biochemistry and Molecular Medicine, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand 3 Nanoelectronics and MEMS Laboratory, National Electronics and Computer Technology Centre, Pathum Thani, 12120, Thailand
* Author to whom correspondence should be addressed.
Received: 11 October 2009 / Revised: 13 November 2009 / Accepted: 30 November 2009 / Published: 10 December 2009
(This article belongs to the Section Chemical Sensors)
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Abstract

An immunoassay performed on a portable microfluidic device was evaluated for the determination of urinary albumin. An increase in absorbance at 500 nm resulting from immunoagglutination was monitored directly on the poly(dimethylsiloxane) (PDMS) microchip using a portable miniature fibre-optic spectrometer. A calibration curve was linear up to 10 mg L–1 (r2 = 0.993), with a detection limit of 0.81 mg L–1 (S/N = 3). The proposed system showed good precision, with relative standard deviations (RSDs) of 5.1%, when evaluated with 10 mg L–1 albumin (n = 10). Determination of urinary albumin with the proposed system gave results highly similar to those determined by the conventional spectrophotometric method using immunoturbidimetric detection (r2 = 0.995; n = 15).
Keywords: microalbuminuria; microfluidic system; on-chip immunoassay; poly(dimethylsiloxane) microalbuminuria; microfluidic system; on-chip immunoassay; poly(dimethylsiloxane)
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Laiwattanapaisal, W.; Songjaroen, T.; Maturos, T.; Lomas, T.; Sappat, A.; Tuantranont, A. On-Chip Immunoassay for Determination of Urinary Albumin. Sensors 2009, 9, 10066-10079.

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