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Sensors 2009, 9(10), 7957-7969; doi:10.3390/s91007957

A Label-Free Electronic Biosensor for Detection of Bone Turnover Markers

1
Nanoworld and Smart Materials and Devices Laboratory, College of Engineering, University of Cincinnati, Cincinnati, OH 45221, USA
2
Environmental Health, College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USA
3
College of Medicine, University of Cincinnati Bone Health and Osteoporosis Center, 222, Piedmont Avenue, Suite 6300, Cincinnati, OH 45219, USA
*
Author to whom correspondence should be addressed.
Received: 29 July 2009 / Revised: 29 September 2009 / Accepted: 30 September 2009 / Published: 12 October 2009
(This article belongs to the Section Biosensors)
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Abstract

This paper describes the development of a biosensor based on label-free immunosensing for the detection of the C-terminal telopeptide bone turnover marker from type-1 collagen. A self-assembled monolayer (SAM) of dithiodipropionic acid was deposited on a gold electrode. Then streptavidin and biotinylated anti-human C-terminal telopeptide antibody were successively conjugated on the self-assembled monolayer. Electrochemical impedance measurements were made to characterize each step of the SAM/streptavidin/biotinylated antibody binding. Subsequently, electrochemical impedance was measured with different concentrations of C-teminal telopeptide. A detection limit of 50 ng/mL and a dynamic range up to 3 μg/mL were achieved. To our knowledge, this is the first attempt to develop a label-free immunosensor based on electrochemical impedance with DC bias for detection of bone-related degradation and rebuilding products. The electronic biosensor might eventually be used for quantitative point-of-care screening of bone health. It is hoped that analysis of bone turnover markers can indicate the beginning of bone diseases such as osteoarthritis and osteoporosis so that treatment might start early when it is most effective. View Full-Text
Keywords: immunosensor; label-free; electrochemical impedance; bone turnover marker immunosensor; label-free; electrochemical impedance; bone turnover marker
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Yun, Y.-H.; Bhattacharya, A.; Watts, N.B.; Schulz, M.J. A Label-Free Electronic Biosensor for Detection of Bone Turnover Markers. Sensors 2009, 9, 7957-7969.

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