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Vasić, V. et al. Na+,K+-ATPase as the Target Enzyme for Organic and Inorganic Compounds. Sensors 2008, 8, 8321-8360
Sensors 2009, 9(1), 378-385; doi:10.3390/s90100378
Article

Immobilization of Electroporated Cells for Fabrication of Cellular Biosensors: Physiological Effects of the Shape of Calcium Alginate Matrices and Foetal Calf Serum

1, 1 and 1,2,*
Received: 19 November 2008 / Revised: 18 December 2008 / Accepted: 26 December 2008 / Published: 9 January 2009
(This article belongs to the Section Biosensors)
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Abstract

In order to investigate the physiological effect of transfected cell immobilization in calcium alginate gels, we immobilized electroporated Vero cells in gels shaped either as spherical beads or as thin membrane layers. In addition, we investigated whether serum addition had a positive effect on cell proliferation and viability in either gel configuration. The gels were stored for four weeks in a medium supplemented or not with 20% (v/v) foetal calf serum. Throughout a culture period of four weeks, cell proliferation and cell viability were assayed by optical microscopy after provision of Trypan Blue. Non-elaborate culture conditions (room temperature, non-CO2 enriched culture atmosphere) were applied throughout the experimental period in order to evaluate cell viability under less than optimal storage conditions. Immobilization of electroporated cells was associated with an initially reduced cell viability, which was gradually increased. Immobilization was associated with maintenance of cell growth for the duration of the experimental period, whereas electroporated cells essentially died after a week in suspension culture. Considerable proliferation of immobilized cells was observed in spherical alginate beads. In both gel configurations, addition of serum was associated with increased cell proliferation. The results of the present study could contribute to an improvement of the storability of biosensors based on electroporated, genetically or membrane-engineered cells.
Keywords: Calcium alginate; Cell immobilization; Electroporation; Foetal calf serum (FCS); Monkey African green kidney (Vero) cells Calcium alginate; Cell immobilization; Electroporation; Foetal calf serum (FCS); Monkey African green kidney (Vero) cells
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Katsanakis, N.; Katsivelis, A.; Kintzios, S. Immobilization of Electroporated Cells for Fabrication of Cellular Biosensors: Physiological Effects of the Shape of Calcium Alginate Matrices and Foetal Calf Serum. Sensors 2009, 9, 378-385.

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