Next Article in Journal
Distributed Principal Component Analysis for Wireless Sensor Networks
Next Article in Special Issue
Utilization of Electrochemical Sensors and Biosensors in Biochemistry and Molecular Biology
Previous Article in Journal
An Interactive Technique for Cartographic Feature Extraction from Aerial and Satellite Image Sensors
Previous Article in Special Issue
Comparison of Mercury Distribution Between Liver and Muscle – A Biomonitoring of Fish from Lightly and Heavily Contaminated Localities
Sensors 2008, 8(8), 4800-4820; doi:10.3390/s8084800
Article

Modelling Amperometric Biosensors Based on Chemically Modified Electrodes

1,2,*  and 3
1 Department of Software Engineering, Vilnius University, Naugarduko 24, LT-03225 Vilnius, Lithuania 2 Institute of Mathematics and Informatics, Akademijos 4, LT-08663 Vilnius, Lithuania 3 Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Sauletekio al. 11, LT-10223 Vilnius, Lithuania
* Author to whom correspondence should be addressed.
Received: 18 April 2008 / Accepted: 28 July 2008 / Published: 19 August 2008
View Full-Text   |   Download PDF [300 KB, uploaded 21 June 2014]   |   Browse Figures

Abstract

The response of an amperometric biosensor based on a chemically modified electrode was modelled numerically. A mathematical model of the biosensor is based on a system of non-linear reaction-diffusion equations. The modelling biosensor comprises two compartments: an enzyme layer and an outer diffusion layer. In order to define the main governing parameters the corresponding dimensionless mathematical model was derived. The digital simulation was carried out using the finite difference technique. The adequacy of the model was evaluated using analytical solutions known for very specific cases of the model parameters. By changing model parameters the output results were numerically analyzed at transition and steady state conditions. The influence of the substrate and mediator concentrations as well as of the thicknesses of the enzyme and diffusion layers on the biosensor response was investigated. Calculations showed complex kinetics of the biosensor response, especially when the biosensor acts under a mixed limitation of the diffusion and the enzyme interaction with the substrate.
Keywords: chemically modified electrode; biosensor; modelling; simulation. chemically modified electrode; biosensor; modelling; simulation.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Baronas, R.; Kulys, J. Modelling Amperometric Biosensors Based on Chemically Modified Electrodes. Sensors 2008, 8, 4800-4820.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert