Sensors 2008, 8(8), 4800-4820; doi:10.3390/s8084800
Article

Modelling Amperometric Biosensors Based on Chemically Modified Electrodes

1,2,* email and 3email
Received: 18 April 2008; Accepted: 28 July 2008 / Published: 19 August 2008
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The response of an amperometric biosensor based on a chemically modified electrode was modelled numerically. A mathematical model of the biosensor is based on a system of non-linear reaction-diffusion equations. The modelling biosensor comprises two compartments: an enzyme layer and an outer diffusion layer. In order to define the main governing parameters the corresponding dimensionless mathematical model was derived. The digital simulation was carried out using the finite difference technique. The adequacy of the model was evaluated using analytical solutions known for very specific cases of the model parameters. By changing model parameters the output results were numerically analyzed at transition and steady state conditions. The influence of the substrate and mediator concentrations as well as of the thicknesses of the enzyme and diffusion layers on the biosensor response was investigated. Calculations showed complex kinetics of the biosensor response, especially when the biosensor acts under a mixed limitation of the diffusion and the enzyme interaction with the substrate.
Keywords: chemically modified electrode; biosensor; modelling; simulation.
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MDPI and ACS Style

Baronas, R.; Kulys, J. Modelling Amperometric Biosensors Based on Chemically Modified Electrodes. Sensors 2008, 8, 4800-4820.

AMA Style

Baronas R, Kulys J. Modelling Amperometric Biosensors Based on Chemically Modified Electrodes. Sensors. 2008; 8(8):4800-4820.

Chicago/Turabian Style

Baronas, Romas; Kulys, Juozas. 2008. "Modelling Amperometric Biosensors Based on Chemically Modified Electrodes." Sensors 8, no. 8: 4800-4820.

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