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Sensors 2008, 8(7), 4413-4428; doi:10.3390/s8074413

Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor

1 Institut FEMTO-ST, Université de Franche Comté, Clinical-Innovation Proteomic Platform, CNRS – 25044 Besançon cedex, France 2 Estrogènes, Expression Génique et Pathologies du Système Nerveux Central, EA3922, IFR 133, Université de Franche-Comté, UFR Sciences et Techniques, 16 route de Gray, 25030 Besançon cedex, France 3 Institut Carnot Bourgogne UMR CNRS 5209, Nanosciences Department, University of Bourgogne, B.P. 47870, 21078 Dijon Cedex, France
* Author to whom correspondence should be addressed.
Received: 12 June 2008 / Revised: 15 July 2008 / Accepted: 25 July 2008 / Published: 28 July 2008
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We constructed an original supramolecular assembly on a surface of sensor composed of an innovative combination of an engineered cytochrome b5 and a modified nucleic acid bound to a synthetic lipid hemimembrane. The protein/DNA block, called (PDNA) 2, was synthesized and purified before its immobilization onto a hybrid bilayer reconstituted on a gold surface. Surface plasmon resonance (SPR) and atomic force microscopy (AFM) were engaged in parallel on the same substrates in order to better understand dynamic events that occur at the surface of the biosensor. Good correlations were obtained in terms of specificity and reversibility. These findings allow us to present a first application of such biosensor in the study of the interaction processes between nuclear receptor and DNA.
Keywords: Nano-objects; molecular lego; AFM; SPR; Protein/DNA interaction. Nano-objects; molecular lego; AFM; SPR; Protein/DNA interaction.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Berthier, A.; Elie-Caille, C.; Lesniewska, E.; Delage-Mourroux, R.; Boireau, W. Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor. Sensors 2008, 8, 4413-4428.

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