Abstract: We constructed an original supramolecular assembly on a surface of sensor composed of an innovative combination of an engineered cytochrome b5 and a modified nucleic acid bound to a synthetic lipid hemimembrane. The protein/DNA block, called (PDNA) 2, was synthesized and purified before its immobilization onto a hybrid bilayer reconstituted on a gold surface. Surface plasmon resonance (SPR) and atomic force microscopy (AFM) were engaged in parallel on the same substrates in order to better understand dynamic events that occur at the surface of the biosensor. Good correlations were obtained in terms of specificity and reversibility. These findings allow us to present a first application of such biosensor in the study of the interaction processes between nuclear receptor and DNA.
Keywords: Nano-objects; molecular lego; AFM; SPR; Protein/DNA interaction.
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Berthier, A.; Elie-Caille, C.; Lesniewska, E.; Delage-Mourroux, R.; Boireau, W. Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor. Sensors 2008, 8, 4413-4428.
Berthier A, Elie-Caille C, Lesniewska E, Delage-Mourroux R, Boireau W. Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor. Sensors. 2008; 8(7):4413-4428.
Berthier, Alexandre; Elie-Caille, Céline; Lesniewska, Eric; Delage-Mourroux, Régis; Boireau, Wilfrid. 2008. "Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor." Sensors 8, no. 7: 4413-4428.