Next Article in Journal
Multi-Source Remotely Sensed Data Combination: Projection Transformation Gap-Fill Procedure
Previous Article in Journal
PAU/RAD: Design and Preliminary Calibration Results of a New L-Band Pseudo-Correlation Radiometer Concept
Sensors 2008, 8(7), 4413-4428; doi:10.3390/s8074413

Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor

1 Institut FEMTO-ST, Université de Franche Comté, Clinical-Innovation Proteomic Platform, CNRS – 25044 Besançon cedex, France 2 Estrogènes, Expression Génique et Pathologies du Système Nerveux Central, EA3922, IFR 133, Université de Franche-Comté, UFR Sciences et Techniques, 16 route de Gray, 25030 Besançon cedex, France 3 Institut Carnot Bourgogne UMR CNRS 5209, Nanosciences Department, University of Bourgogne, B.P. 47870, 21078 Dijon Cedex, France
* Author to whom correspondence should be addressed.
Received: 12 June 2008 / Revised: 15 July 2008 / Accepted: 25 July 2008 / Published: 28 July 2008
View Full-Text   |   Download PDF [625 KB, uploaded 21 June 2014]   |   Browse Figures


We constructed an original supramolecular assembly on a surface of sensor composed of an innovative combination of an engineered cytochrome b5 and a modified nucleic acid bound to a synthetic lipid hemimembrane. The protein/DNA block, called (PDNA) 2, was synthesized and purified before its immobilization onto a hybrid bilayer reconstituted on a gold surface. Surface plasmon resonance (SPR) and atomic force microscopy (AFM) were engaged in parallel on the same substrates in order to better understand dynamic events that occur at the surface of the biosensor. Good correlations were obtained in terms of specificity and reversibility. These findings allow us to present a first application of such biosensor in the study of the interaction processes between nuclear receptor and DNA.
Keywords: Nano-objects; molecular lego; AFM; SPR; Protein/DNA interaction. Nano-objects; molecular lego; AFM; SPR; Protein/DNA interaction.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Berthier, A.; Elie-Caille, C.; Lesniewska, E.; Delage-Mourroux, R.; Boireau, W. Nanobioengineering and Characterization of a Novel Estrogen Receptor Biosensor. Sensors 2008, 8, 4413-4428.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert