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Sensors 2008, 8(5), 3106-3122; doi:10.3390/s8053106
Article

Utilizing of Adsorptive Transfer Stripping Technique Brdicka Reaction for Determination of Metallothioneins Level in Melanoma Cells, Blood Serum and Tissues

1, 1, 1,2, 3, 3, 4, 5, 6, 6 and 1,*
Received: 22 April 2008; Accepted: 9 May 2008 / Published: 10 May 2008
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Abstract: In the paper we utilized the adsorptive transfer stripping differential pulse voltammetry Brdicka reaction for the determination of metallothioneins (MT) in melanoma cells, animal melanoma tissues (MeLiM miniature pig) and blood serum of patients with malignant melanoma. Primarily we attempted to investigate the influence of dilution of real sample on MT electrochemical response. Dilution of samples of 1 000 times was chosen the most suitable for determination of MT level in biological samples. Then we quantified the MT level in the melanoma cells, the animal melanoma tissues and the blood serum samples. The MT content in the cells varied within the range from 4.2 to 11.2 μM. At animal melanoma tissues (melanomas localized on abdomen, back limb and dorsum) the highest content of MT was determined in the tumour sampled on the back of the animal and was nearly 500 μg of MTs per gram of a tissue. We also quantified content of MT in metastases, which was found in liver, spleen and lymph nodes. Moreover the average MT level in the blood serum samples from patients with melanoma was 3.0 ± 0.8 μM. MT levels determined at melanoma samples were significantly (p < 0.05) higher compared to control ones at cells, tissues and blood serum.
Keywords: Metallothionein; Protein; Tumour Marker; Cell; Animal Tissue; Human blood serum; Differential Pulse Voltammetry; Adsorptive Transfer Stripping Technique; Brdicka Reaction Metallothionein; Protein; Tumour Marker; Cell; Animal Tissue; Human blood serum; Differential Pulse Voltammetry; Adsorptive Transfer Stripping Technique; Brdicka Reaction
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Krizkova, S.; Fabrik, I.; Adam, V.; Kukacka, J.; Prusa, R.; Chavis, G.J.; Trnkova, L.; Strnadel, J.; Horak, V.; Kizek, R. Utilizing of Adsorptive Transfer Stripping Technique Brdicka Reaction for Determination of Metallothioneins Level in Melanoma Cells, Blood Serum and Tissues. Sensors 2008, 8, 3106-3122.

AMA Style

Krizkova S, Fabrik I, Adam V, Kukacka J, Prusa R, Chavis GJ, Trnkova L, Strnadel J, Horak V, Kizek R. Utilizing of Adsorptive Transfer Stripping Technique Brdicka Reaction for Determination of Metallothioneins Level in Melanoma Cells, Blood Serum and Tissues. Sensors. 2008; 8(5):3106-3122.

Chicago/Turabian Style

Krizkova, Sona; Fabrik, Ivo; Adam, Vojtech; Kukacka, Jiri; Prusa, Richard; Chavis, Grace J.; Trnkova, Libuse; Strnadel, Jan; Horak, Vratislav; Kizek, Rene. 2008. "Utilizing of Adsorptive Transfer Stripping Technique Brdicka Reaction for Determination of Metallothioneins Level in Melanoma Cells, Blood Serum and Tissues." Sensors 8, no. 5: 3106-3122.


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