Next Article in Journal
An Electrochemical DNA Biosensor Developed on a Nanocomposite Platform of Gold and Poly(propyleneimine) Dendrimer
Next Article in Special Issue
Recent Progress in Nucleic Acid Aptamer-Based Biosensors and Bioassays
Previous Article in Journal
Linear FBG Temperature Sensor Interrogation with Fabry-Perot ITU Multi-wavelength Reference
Previous Article in Special Issue
Molecular Recognition and Specific Interactions for Biosensing Applications
Sensors 2008, 8(10), 6777-6790; doi:10.3390/s8106777
Article

Interfacial Recognition of Acetylcholine by an Amphiphilic p-Sulfonatocalix[8]arene Derivative Incorporated into Dimyristoyl Phosphatidylcholine Vesicles

1,2,* , 1 and 3
1 Nano-bio Materials Laboratory, Immunology Frontier Research Center, Osaka University, Suita, Osaka, 565-0871, Japan 2 Graduate School of Frontier Biosciences, High Performance Bioimaging Facility, Suita, Osaka University, Osaka 565-0871, Japan 3 Division of Pathogenesis and Control of Oral Disease, Graduate School of Dentistry, Osaka University, Suita, Osaka, 565-0781, Japan
* Author to whom correspondence should be addressed.
Received: 23 August 2008 / Revised: 17 October 2008 / Accepted: 23 October 2008 / Published: 29 October 2008
(This article belongs to the Special Issue Molecular Recognition and Sensors, Including Molecular Imprinting)
View Full-Text   |   Download PDF [580 KB, uploaded 21 June 2014]   |   Browse Figures

Abstract

Dodecyl ether derivatives 1-3 of p-sulfonatocalix[n]arene were incorporated into dimyristoyl phosphatidylcholine (DMPC) vesicles, and their binding abilities for acetylcholine (ACh) were examined by using steady-state fluorescence/fluorescence anisotropy and fluorescence correlation spectroscopy (FCS). For the detection of ACh binding to the DMPC vesicles containing 5 mol % of 1-3, competitive fluorophore displacement experiments were performed, where rhodamine 6G (Rh6G) was used as a fluorescent guest. The addition of Rh6G to the DMPC vesicles containing 3 resulted in a decrease in the fluorescence intensity of Rh6G with an increase of its fluorescence anisotropy, indicating that Rh6G binds to the DMPC-3 vesicles. In the case of DMPC-1 and DMPC-2 vesicles, significant changes in the fluorescence spectra of Rh6G were not observed. When ACh was added to the DMPC-3 vesicles in the presence of Rh6G ([3]/[Rh6G]=100), the fluorescence intensity of Rh6G increased with a decrease in its fluorescence anisotropy. From the analysis of fluorescence titration data, the association constants were determined to be 7.1×105 M-1 for Rh6G-3 complex and 1.1×102 M-1 for ACh-3 complex at the DMPC-3 vesicles. To get a direct evidence for the binding of Rh6G and its displacement by ACh at the DMPC-3 vesicles, diffusion times of the Rh6G were measured by using FCS. Binding selectivity of the DMPC-3 vesicles for ACh, choline, GABA, L-aspartic acid, L-glutamic acid, L-arginine, L-lysine, L-histamine and ammonium chloride was also evaluated using FCS.
Keywords: Interfacial recognition; acetylcholine; sulfonatocalixarene; lipid bilayer; vesicle; fluorescence correlation spectroscopy Interfacial recognition; acetylcholine; sulfonatocalixarene; lipid bilayer; vesicle; fluorescence correlation spectroscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Jin, T.; Fujii, F.; Ooi, Y. Interfacial Recognition of Acetylcholine by an Amphiphilic p-Sulfonatocalix[8]arene Derivative Incorporated into Dimyristoyl Phosphatidylcholine Vesicles. Sensors 2008, 8, 6777-6790.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

Cited By

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert