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Sensors 2008, 8(10), 6453-6470; doi:10.3390/s8106453
Article

Detection of Staphylococcus epidermidis by a Quartz Crystal Microbalance Nucleic Acid Biosensor Array Using Au Nanoparticle Signal Amplification

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Received: 2 September 2008; in revised form: 3 October 2008 / Accepted: 17 October 2008 / Published: 21 October 2008
(This article belongs to the Section Biosensors)
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Abstract: Staphylococcus epidermidis is a critical pathogen of nosocomial blood infections, resulting in significant morbidity and mortality. A piezoelectric quartz crystal microbalance (QCM) nucleic acid biosensor array using Au nanoparticle signal amplification was developed to rapidly detect S. epidermidis in clinical samples. The synthesized thiolated probes specific targeting S. epidermidis 16S rRNA gene were immobilized on the surface of QCM nucleic acid biosensor arrays. Hybridization was induced by exposing the immobilized probes to the PCR amplified fragments of S. epidermidis, resulting in a mass change and a consequent frequency shift of the QCM biosensor. To further enhance frequency shift results from above described hybridizations, streptavidin coated Au nanoparticles were conjugated to the PCR amplified fragments. The results showed that the lowest detection limit of current QCM system was 1.3×103 CFU/mL. A linear correlation was found when the concentration of S. epidermidis varied from 1.3×103 to 1.3×107 CFU/mL. In addition, 55 clinical samples were detected with both current QCM biosensor system and conventional clinical microbiological method, and the sensitivity and specificity of current QCM biosensor system were 97.14% and 100%, respectively. In conclusion, the current QCM system is a rapid, low-cost and sensitive method that can be used to identify infection of S. epidermidis in clinical samples.
Keywords: Au nanoparticle; biosensor; S. epidermidis; quartz crystal microbalance Au nanoparticle; biosensor; S. epidermidis; quartz crystal microbalance
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Xia, H.; Wang, F.; Huang, Q.; Huang, J.; Chen, M.; Wang, J.; Yao, C.; Chen, Q.; Cai, G.; Fu, W. Detection of Staphylococcus epidermidis by a Quartz Crystal Microbalance Nucleic Acid Biosensor Array Using Au Nanoparticle Signal Amplification. Sensors 2008, 8, 6453-6470.

AMA Style

Xia H, Wang F, Huang Q, Huang J, Chen M, Wang J, Yao C, Chen Q, Cai G, Fu W. Detection of Staphylococcus epidermidis by a Quartz Crystal Microbalance Nucleic Acid Biosensor Array Using Au Nanoparticle Signal Amplification. Sensors. 2008; 8(10):6453-6470.

Chicago/Turabian Style

Xia, Han; Wang, Feng; Huang, Qing; Huang, Junfu; Chen, Ming; Wang, Jue; Yao, Chunyan; Chen, Qinghai; Cai, Guoru; Fu, Weiling. 2008. "Detection of Staphylococcus epidermidis by a Quartz Crystal Microbalance Nucleic Acid Biosensor Array Using Au Nanoparticle Signal Amplification." Sensors 8, no. 10: 6453-6470.



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