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Sensors 2008, 8(10), 6453-6470; doi:10.3390/s8106453

Detection of Staphylococcus epidermidis by a Quartz Crystal Microbalance Nucleic Acid Biosensor Array Using Au Nanoparticle Signal Amplification

1
Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, P.R China
2
The 26th Research Institute, Chinese Electronic Scientific and Technical Group Company, Chongqing 400060, P.R China
*
Author to whom correspondence should be addressed.
Received: 2 September 2008 / Revised: 3 October 2008 / Accepted: 17 October 2008 / Published: 21 October 2008
(This article belongs to the Section Biosensors)
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Abstract

Staphylococcus epidermidis is a critical pathogen of nosocomial blood infections, resulting in significant morbidity and mortality. A piezoelectric quartz crystal microbalance (QCM) nucleic acid biosensor array using Au nanoparticle signal amplification was developed to rapidly detect S. epidermidis in clinical samples. The synthesized thiolated probes specific targeting S. epidermidis 16S rRNA gene were immobilized on the surface of QCM nucleic acid biosensor arrays. Hybridization was induced by exposing the immobilized probes to the PCR amplified fragments of S. epidermidis, resulting in a mass change and a consequent frequency shift of the QCM biosensor. To further enhance frequency shift results from above described hybridizations, streptavidin coated Au nanoparticles were conjugated to the PCR amplified fragments. The results showed that the lowest detection limit of current QCM system was 1.3×103 CFU/mL. A linear correlation was found when the concentration of S. epidermidis varied from 1.3×103 to 1.3×107 CFU/mL. In addition, 55 clinical samples were detected with both current QCM biosensor system and conventional clinical microbiological method, and the sensitivity and specificity of current QCM biosensor system were 97.14% and 100%, respectively. In conclusion, the current QCM system is a rapid, low-cost and sensitive method that can be used to identify infection of S. epidermidis in clinical samples. View Full-Text
Keywords: Au nanoparticle; biosensor; S. epidermidis; quartz crystal microbalance Au nanoparticle; biosensor; S. epidermidis; quartz crystal microbalance
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MDPI and ACS Style

Xia, H.; Wang, F.; Huang, Q.; Huang, J.; Chen, M.; Wang, J.; Yao, C.; Chen, Q.; Cai, G.; Fu, W. Detection of Staphylococcus epidermidis by a Quartz Crystal Microbalance Nucleic Acid Biosensor Array Using Au Nanoparticle Signal Amplification. Sensors 2008, 8, 6453-6470.

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