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Sensors 2008, 8(1), 582-593; doi:10.3390/s8010582
Article

Using Monoclonal Antibody to Determine Lead Ions with a Localized Surface Plasmon Resonance Fiber-optic Biosensor

*  and
Received: 4 October 2007; Accepted: 24 January 2008 / Published: 25 January 2008
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Abstract: A novel reflection-based localized surface plasmon resonance (LSPR) fiber-optic probe has been developed to determine the heavy metal lead ion concentration. Monoclonal antibody as the detecting probe containing massive amino groups to capture Pb(II)-chelate complexes was immobilized onto gold nanoparticle-modified optical fiber (NMAuOF). The optimal immobilizing conditions of monoclonal antibody on to the NMAuOF are 189 μg/mL in pH7.4 PBS for 2 h at 25°C. The absorbability of the functionalized NMAuOF sensor increases to 12.2 % upon changing the Pb(II)-EDTA level from 10 to 100 ppb with a detection limit of 0.27 ppb. The sensor retains 92.7 % of its original activity and gives reproducible results after storage in 5% D-( )-Trehalose dehydrate solution at 4°C for 35 days. In conclusion, the monoclonal antibody-functionalized NMAuOF sensor shows a promising result for determining the concentration of Pb(II) with high sensitivity.
Keywords: localized surface plasmon resonance; biosensor; gold nanoparticle; monoclonal antibody; lead-chelate complex. antibody; lead-chelate complex localized surface plasmon resonance; biosensor; gold nanoparticle; monoclonal antibody; lead-chelate complex. antibody; lead-chelate complex
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lin, T.-J.; Chung, M.-F. Using Monoclonal Antibody to Determine Lead Ions with a Localized Surface Plasmon Resonance Fiber-optic Biosensor. Sensors 2008, 8, 582-593.

AMA Style

Lin T-J, Chung M-F. Using Monoclonal Antibody to Determine Lead Ions with a Localized Surface Plasmon Resonance Fiber-optic Biosensor. Sensors. 2008; 8(1):582-593.

Chicago/Turabian Style

Lin, Tsao-Jen; Chung, Mon-Fu. 2008. "Using Monoclonal Antibody to Determine Lead Ions with a Localized Surface Plasmon Resonance Fiber-optic Biosensor." Sensors 8, no. 1: 582-593.



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