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Piezoelectric Sensor for Determination of Genetically Modified Soybean Roundup Readyâ in Samples not Amplified by PCR
Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-474 Olsztyn, Poland
Genetic Modifications Analysis Laboratory, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5a, 02-106 WARSAW, Poland
Institute of Genetics and Biotechnology, Warsaw University, Pawińskiego 5a, 02-106 Warsaw, Poland
* Author to whom correspondence should be addressed.
Received: 19 July 2007; Accepted: 10 August 2007 / Published: 14 August 2007
Abstract: The chemically modified piezoelectrodes were utilized to develop relativelycheap and easy to use biosensor for determination of genetically modified Roundup Readysoybean (RR soybean). The biosensor relies on the immobilization onto goldpiezoelectrodes of the 21-mer single stranded oligonucleotide (probes) related to5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) gene, which is an active componentof an insert integrated into RR soybean genome. The hybridization reaction between theprobe and the target complementary sequence in solution was monitored. The system wasoptimized using synthetic oligonucleotides, which were applied for EPSPS gene detectionin DNA samples extracted from animal feed containing 30% RR soybean amplified by thePCR and nonamplified by PCR. The detection limit for genomic DNA was in the range of4.7·105 numbers of genom copies contained EPSPS gene in the QCM cell. The propertiessuch as sensitivity and selectivity of piezoelectric senor presented here indicated that it could be applied for the direct determination of genetically modified RR soybean in the samples non-amplified by PCR.
Keywords: Piezoelectrodes; Roundup Ready soybean; Genomic DNA.
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MDPI and ACS Style
Stobiecka, M.; Cieśla, J.M.; Janowska, B.; Tudek, B.; Radecka, H. Piezoelectric Sensor for Determination of Genetically Modified Soybean Roundup Readyâ in Samples not Amplified by PCR. Sensors 2007, 7, 1462-1479.
Stobiecka M, Cieśla JM, Janowska B, Tudek B, Radecka H. Piezoelectric Sensor for Determination of Genetically Modified Soybean Roundup Readyâ in Samples not Amplified by PCR. Sensors. 2007; 7(8):1462-1479.
Stobiecka, Magdalena; Cieśla, Jarosław M.; Janowska, Beata; Tudek, Barbara; Radecka, Hanna. 2007. "Piezoelectric Sensor for Determination of Genetically Modified Soybean Roundup Readyâ in Samples not Amplified by PCR." Sensors 7, no. 8: 1462-1479.