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Sensors 2007, 7(7), 1091-1107; doi:10.3390/s7071091
Article

Comparative Study of Protein Immobilization Properties on Calixarene Monolayers

1
,
2
,
3, 3
,
4
,
4
,
5
 and
5,*
1 College of Pharmacy, Pusan National University, Pusan 609-735, Korea 2 Seed Biochips, Chung-Buk, 363-883, Korea 3 Department of Molecular Science and Technology, Ajou University, Suwon 442-749, Korea 4 Department of Textile System Engineering, Kyungpook National University, Taegu 702-701, Korea 5 Department of Nanomedical Engineering, Pusan National University, Pusan 609-735, Korea
* Author to whom correspondence should be addressed.
Received: 19 June 2007 / Accepted: 28 June 2007 / Published: 29 June 2007
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Abstract

Three calix[4]arene (Cal-4) derivatives of which contain ethylester (1),carboxylic acid (2), and crownether (3) at the lower rim with a common reactive thiol at theupper rim were synthesized and constructed to self-assembled monolayers (SAMs) on Aufilms. After spectroscopic characterization of monolayers, the interaction between Cal-4and surface confined bovine serum albumin (BSA) in the SAMs was analyzed by surfaceplasmon resonance (SPR). The estimated surface concentration of BSA on the Cal-4 SAMwith crownether group was the highest among the three Cal-4 derivatives. Anti-hIgG andhIgG pair was employed for the investigation of protein-protein interaction. Molecularinteraction between anti-hIgG and hIgG can be detected in a concentration range of 10pg/mL to 200 pg/mL on the Cal-4 derivative 3 SAM modified SPR chip.
Keywords: Calix[4]arene; Self-assembled monolayer; Linker system; anti-hIgG; Biosensor. Calix[4]arene; Self-assembled monolayer; Linker system; anti-hIgG; Biosensor.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Chen, H.; Lee, M.; Choi, S.; Kim, J.-H.; Choi, H.-J.; Kim, S.-H.; Lee, J.; Koh, K. Comparative Study of Protein Immobilization Properties on Calixarene Monolayers. Sensors 2007, 7, 1091-1107.

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