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Comparative Study of Protein Immobilization Properties on Calixarene Monolayers
College of Pharmacy, Pusan National University, Pusan 609-735, Korea
Seed Biochips, Chung-Buk, 363-883, Korea
Department of Molecular Science and Technology, Ajou University, Suwon 442-749, Korea
Department of Textile System Engineering, Kyungpook National University, Taegu 702-701, Korea
Department of Nanomedical Engineering, Pusan National University, Pusan 609-735, Korea
* Author to whom correspondence should be addressed.
Received: 19 June 2007; Accepted: 28 June 2007 / Published: 29 June 2007
Abstract: Three calixarene (Cal-4) derivatives of which contain ethylester (1),carboxylic acid (2), and crownether (3) at the lower rim with a common reactive thiol at theupper rim were synthesized and constructed to self-assembled monolayers (SAMs) on Aufilms. After spectroscopic characterization of monolayers, the interaction between Cal-4and surface confined bovine serum albumin (BSA) in the SAMs was analyzed by surfaceplasmon resonance (SPR). The estimated surface concentration of BSA on the Cal-4 SAMwith crownether group was the highest among the three Cal-4 derivatives. Anti-hIgG andhIgG pair was employed for the investigation of protein-protein interaction. Molecularinteraction between anti-hIgG and hIgG can be detected in a concentration range of 10pg/mL to 200 pg/mL on the Cal-4 derivative 3 SAM modified SPR chip.
Keywords: Calixarene; Self-assembled monolayer; Linker system; anti-hIgG; Biosensor.
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MDPI and ACS Style
Chen, H.; Lee, M.; Choi, S.; Kim, J.-H.; Choi, H.-J.; Kim, S.-H.; Lee, J.; Koh, K. Comparative Study of Protein Immobilization Properties on Calixarene Monolayers. Sensors 2007, 7, 1091-1107.
Chen H, Lee M, Choi S, Kim J-H, Choi H-J, Kim S-H, Lee J, Koh K. Comparative Study of Protein Immobilization Properties on Calixarene Monolayers. Sensors. 2007; 7(7):1091-1107.
Chen, Hongxia; Lee, Minsu; Choi, Sungwook; Kim, Jae-Ho; Choi, Heung-Jin; Kim, Sung-Hoon; Lee, Jeabeom; Koh, Kwangnak. 2007. "Comparative Study of Protein Immobilization Properties on Calixarene Monolayers." Sensors 7, no. 7: 1091-1107.