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Sensors 2007, 7(5), 719-729; doi:10.3390/s7050719

Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film Electrodes–fabrication and Evaluation as Hydrogen Peroxide Sensor

1
Department of Chemistry, BK 21 NanoBiosensor Research Team, Hannam University, Daejeon 305-811, Republic of Korea
2
Department of Chemistry Graduate School, Kyungpook National University, Daegu 702-701, Republic of Korea
3
Korea Research Research Institute of Bioscience & Biotechnology, Daejeon 305-333, Republic of Korea
*
Author to whom correspondence should be addressed.
Received: 17 April 2007 / Accepted: 29 May 2007 / Published: 31 May 2007
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Abstract

Enzyme modified electrodes were fabricated with poly(aniline-co-m-aminophenol). Electrochemical polymerization of aniline and m-aminophenol wasperformed to get the film of copolymer on the surface of gold electrode. Modifiedelectrodes were fabricated by two methods, physical entrapment and covalent cross-linking.In one of the method, gold nanoparticles were loaded into the copolymer film andhorseradish peroxidase (HRP) was immobilized into the Au nanoparticle loaded copolymerfilm through physical entrapment. In the other method, the amino and -OH groups in thecopolymer are utilized to form covalent functionalization with HRP via glutaric dialdehydeas cross-linker/mediator. The conducting copolymer/enzyme modified electrodes preparedby physical entrapment/covalent functionalization of enzyme were tested forelectrocatalytic activities towards sensing of H2O2. Amperometric results indicate thatenzyme modified electrode via physical entrapment possesses better electrocatalyticperformance over covalent functionalized enzyme electrode. View Full-Text
Keywords: horseradish peroxidase; poly(aniline-co-m-aminophenol); Au nanoparticles; physical adsorption; covalent bonding horseradish peroxidase; poly(aniline-co-m-aminophenol); Au nanoparticles; physical adsorption; covalent bonding
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MDPI and ACS Style

Seo, K.-D.; Lee, K.-P.; Gopalan, A.I.; Chung, S.J.; Lim, Y.T.; Choi, S.-H. Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film Electrodes–fabrication and Evaluation as Hydrogen Peroxide Sensor. Sensors 2007, 7, 719-729.

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