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• Yang, X
• Yuan, H
• Zhou, Z
• Xiao, D
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• Yuan, H
• Zhou, Z
• Xiao, D
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• Yang, X
• Yuan, H
• Zhou, Z
• Xiao, D

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Sensors 2007, 7(5), 708-718; doi:10.3390/s7050708

Article

Preparation of Silver Nanoparticle and Its Application to the Determination of ct-DNA

Chunhua Liu ¹ , Xiupei Yang ¹ , Hongyan Yuan ² , Zaide Zhou ¹  and Dan Xiao ^1,*

¹ College of Chemistry, Sichuan University, Chengdu 610064, P. R. China ² College of Chemical Engineering, Sichuan University, Chengdu 610064, P. R. China ³ College of Chemistry and Chemical Engineering, China West Normal University, Nanchong, 637002, P. R. China

* Author to whom correspondence should be addressed.

Received: 16 March 2007 / Accepted: 29 May 2007 / Published: 31 May 2007

(This article belongs to the Special Issue Optical Biosensors)

Download PDF Full-Text [881 KB, uploaded 20 June 2008 16:51 CEST]

Abstract: A new method to prepare silver nanoparticles was reported in this paper, whichbased on aqueous-gaseous phase reaction of silver nitrate solution and ammonia gas. Theproposed method is effective, rapid, and convenient. Transmission electron microscope(TEM) and X-ray diffraction (XRD) were used to characterize the structure of the particles,respectively. It was found that the average diameter of the particles was about 10 nm and theshape was spherical. And the strong fluorescence signal of the silver nanoparticles solutioncan be quenched after the adding of the calf thymus DNA (ct-DNA) solution. Thequenched fluorescence intensity was linear with the concentration of ct-DNA in the range of0.5 to 5.0 × 10⁴ ng mL^-1 with a detection limit of 0.3 ng mL^-1.

Keywords: silver; nanoparticle; fluorescence; calf thymus DNA (ct-DNA)

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