Sensors 2007, 7(5), 708-718; doi:10.3390/s7050708
Article

Preparation of Silver Nanoparticle and Its Application to the Determination of ct-DNA

1, 1, 2, 1 and 1,* email
Received: 16 March 2007; Accepted: 29 May 2007 / Published: 31 May 2007
(This article belongs to the Special Issue Optical Biosensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: A new method to prepare silver nanoparticles was reported in this paper, whichbased on aqueous-gaseous phase reaction of silver nitrate solution and ammonia gas. Theproposed method is effective, rapid, and convenient. Transmission electron microscope(TEM) and X-ray diffraction (XRD) were used to characterize the structure of the particles,respectively. It was found that the average diameter of the particles was about 10 nm and theshape was spherical. And the strong fluorescence signal of the silver nanoparticles solutioncan be quenched after the adding of the calf thymus DNA (ct-DNA) solution. Thequenched fluorescence intensity was linear with the concentration of ct-DNA in the range of0.5 to 5.0 × 104 ng mL-1 with a detection limit of 0.3 ng mL-1.
Keywords: silver; nanoparticle; fluorescence; calf thymus DNA (ct-DNA)
PDF Full-text Download PDF Full-Text [881 KB, uploaded 21 June 2014 00:32 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Liu, C.; Yang, X.; Yuan, H.; Zhou, Z.; Xiao, D. Preparation of Silver Nanoparticle and Its Application to the Determination of ct-DNA. Sensors 2007, 7, 708-718.

AMA Style

Liu C, Yang X, Yuan H, Zhou Z, Xiao D. Preparation of Silver Nanoparticle and Its Application to the Determination of ct-DNA. Sensors. 2007; 7(5):708-718.

Chicago/Turabian Style

Liu, Chunhua; Yang, Xiupei; Yuan, Hongyan; Zhou, Zaide; Xiao, Dan. 2007. "Preparation of Silver Nanoparticle and Its Application to the Determination of ct-DNA." Sensors 7, no. 5: 708-718.


Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert