Sensors 2007, 7(12), 3472-3480; doi:10.3390/s7123472
Article

Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optical Technique and Respiration Activity MOnitoring System (RAMOS)

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Received: 3 December 2007; Accepted: 20 December 2007 / Published: 20 December 2007
(This article belongs to the Special Issue State-of-the-Art Polymer Based pH Sensors)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Shake flasks are commonly used for process development in biotechnologyindustry. For this purpose a lot of information is required from the growth conditions duringthe fermentation experiments. Therefore, Anderlei et al. developed the RAMOS technology[1, 2], which proviedes on-line oxygen and carbondioxide transfer rates in shake flasks.Besides oxygen consumption, the pH in the medium also plays an important role for thesuccessful cultivation of micro-organisms and for process development. For online pHmeasurement fiber optical methods based on fluorophores are available. Here a combinationof the on-line Oxygen Transfer Rate (OTR) measurements in the RAMOS device with anon-line, fiber optical pH measurement is presented. To demonstrate the application of thecombined measurement techniques, Escherichia coli cultivations were performed and on-line pH measurements were compared with off-line samples. The combination of on-lineOTR and pH measurements gives a lot of information about the cultivation and, therefore, itis a powerful technique for monitoring shake flask experiments as well as for processdevelopment.
Keywords: fiber optical pH measurement; shaking flask; oxygen transfer rate; RAMOS; E. coli; process development
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MDPI and ACS Style

Scheidle, M.; Klinger, J.; Büchs, J. Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optical Technique and Respiration Activity MOnitoring System (RAMOS). Sensors 2007, 7, 3472-3480.

AMA Style

Scheidle M, Klinger J, Büchs J. Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optical Technique and Respiration Activity MOnitoring System (RAMOS). Sensors. 2007; 7(12):3472-3480.

Chicago/Turabian Style

Scheidle, Marco; Klinger, Johannes; Büchs, Jochen. 2007. "Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optical Technique and Respiration Activity MOnitoring System (RAMOS)." Sensors 7, no. 12: 3472-3480.

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