Sensors 2007, 7(12), 3472-3480; doi:10.3390/s7123472
Article

Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optical Technique and Respiration Activity MOnitoring System (RAMOS)

Biochemical Engineering, RWTH Aachen University, Sammelbau Biologie, Worringerweg 1, D-52074 Aachen, Germany
* Author to whom correspondence should be addressed.
Received: 3 December 2007; Accepted: 20 December 2007 / Published: 20 December 2007
(This article belongs to the Special Issue State-of-the-Art Polymer Based pH Sensors)
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Abstract: Shake flasks are commonly used for process development in biotechnologyindustry. For this purpose a lot of information is required from the growth conditions duringthe fermentation experiments. Therefore, Anderlei et al. developed the RAMOS technology[1, 2], which proviedes on-line oxygen and carbondioxide transfer rates in shake flasks.Besides oxygen consumption, the pH in the medium also plays an important role for thesuccessful cultivation of micro-organisms and for process development. For online pHmeasurement fiber optical methods based on fluorophores are available. Here a combinationof the on-line Oxygen Transfer Rate (OTR) measurements in the RAMOS device with anon-line, fiber optical pH measurement is presented. To demonstrate the application of thecombined measurement techniques, Escherichia coli cultivations were performed and on-line pH measurements were compared with off-line samples. The combination of on-lineOTR and pH measurements gives a lot of information about the cultivation and, therefore, itis a powerful technique for monitoring shake flask experiments as well as for processdevelopment.
Keywords: fiber optical pH measurement; shaking flask; oxygen transfer rate; RAMOS; E. coli; process development

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MDPI and ACS Style

Scheidle, M.; Klinger, J.; Büchs, J. Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optical Technique and Respiration Activity MOnitoring System (RAMOS). Sensors 2007, 7, 3472-3480.

AMA Style

Scheidle M, Klinger J, Büchs J. Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optical Technique and Respiration Activity MOnitoring System (RAMOS). Sensors. 2007; 7(12):3472-3480.

Chicago/Turabian Style

Scheidle, Marco; Klinger, Johannes; Büchs, Jochen. 2007. "Combination of On-line pH and Oxygen Transfer Rate Measurement in Shake Flasks by Fiber Optical Technique and Respiration Activity MOnitoring System (RAMOS)." Sensors 7, no. 12: 3472-3480.

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