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Sensors 2007, 7(11), 2808-2824; doi:10.3390/s7112808
Article

Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins

, , ,  and *
Received: 24 October 2007; Accepted: 14 November 2007 / Published: 16 November 2007
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Abstract: Many organisms secrete antimicrobial peptides (AMPs) for protection againstharmful microbes. The present study describes detection of botulinum neurotoxoids A, Band E using AMPs as recognition elements in an array biosensor. While AMP affinitieswere similar to those for anti-botulinum antibodies, differences in binding patterns wereobserved and can potentially be used for identification of toxoid serotype. Furthermore,some AMPs also demonstrated superior detection sensitivity compared to antibodies: toxoidA could be detected at 3.5 LD50 of the active toxin in a 75-min assay, whereas toxoids B andE were detected at 14 and 80 LD50 for their respective toxins.
Keywords: Antimicrobial peptide; botulinum toxin; detection; array; biosensor Antimicrobial peptide; botulinum toxin; detection; array; biosensor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kulagina, N.V.; Anderson, G.P.; Ligler, F.S.; Shaffer, K.M.; Taitt, C.R. Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins. Sensors 2007, 7, 2808-2824.

AMA Style

Kulagina NV, Anderson GP, Ligler FS, Shaffer KM, Taitt CR. Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins. Sensors. 2007; 7(11):2808-2824.

Chicago/Turabian Style

Kulagina, Nadezhda V.; Anderson, George P.; Ligler, Frances S.; Shaffer, Kara M.; Taitt, Chris R. 2007. "Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins." Sensors 7, no. 11: 2808-2824.


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