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• Kulagina, NV.
• Anderson, GP.
• Ligler, FS.
• Shaffer, KM.
• Taitt, CRowe
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• Kulagina, NV.
• Anderson, GP.
• Ligler, FS.
• Shaffer, KM.
• Taitt, CRowe
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• Kulagina, NV.
• Anderson, GP.
• Ligler, FS.
• Shaffer, KM.
• Taitt, CRowe

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Sensors 2007, 7(11), 2808-2824; doi:10.3390/s7112808

Article

Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins

Nadezhda V. Kulagina, George P. Anderson, Frances S. Ligler, Kara M. Shaffer and Chris Rowe Taitt*

Center for Bio/Molecular Science & Engineering, Code 6900, Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA

* Author to whom correspondence should be addressed.

Received: 24 October 2007 / Accepted: 14 November 2007 / Published: 16 November 2007

(This article belongs to the Special Issue Photonic Sensors for Chemical, Biological, and Nuclear Agent Detection)

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Abstract: Many organisms secrete antimicrobial peptides (AMPs) for protection againstharmful microbes. The present study describes detection of botulinum neurotoxoids A, Band E using AMPs as recognition elements in an array biosensor. While AMP affinitieswere similar to those for anti-botulinum antibodies, differences in binding patterns wereobserved and can potentially be used for identification of toxoid serotype. Furthermore,some AMPs also demonstrated superior detection sensitivity compared to antibodies: toxoidA could be detected at 3.5 LD₅₀ of the active toxin in a 75-min assay, whereas toxoids B andE were detected at 14 and 80 LD₅₀ for their respective toxins.

Keywords: Antimicrobial peptide; botulinum toxin; detection; array; biosensor

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