Abstract: Many organisms secrete antimicrobial peptides (AMPs) for protection againstharmful microbes. The present study describes detection of botulinum neurotoxoids A, Band E using AMPs as recognition elements in an array biosensor. While AMP affinitieswere similar to those for anti-botulinum antibodies, differences in binding patterns wereobserved and can potentially be used for identification of toxoid serotype. Furthermore,some AMPs also demonstrated superior detection sensitivity compared to antibodies: toxoidA could be detected at 3.5 LD50 of the active toxin in a 75-min assay, whereas toxoids B andE were detected at 14 and 80 LD50 for their respective toxins.
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Kulagina, N.V.; Anderson, G.P.; Ligler, F.S.; Shaffer, K.M.; Taitt, C.R. Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins. Sensors 2007, 7, 2808-2824.
Kulagina NV, Anderson GP, Ligler FS, Shaffer KM, Taitt CR. Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins. Sensors. 2007; 7(11):2808-2824.
Kulagina, Nadezhda V.; Anderson, George P.; Ligler, Frances S.; Shaffer, Kara M.; Taitt, Chris R. 2007. "Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins." Sensors 7, no. 11: 2808-2824.