Next Article in Journal
Piezoelectric Biosensor for a Simple Serological Diagnosis of Tularemia in Infected European Brown Hares (Lepus europaeus)
Next Article in Special Issue
Fiber Optic Sensors For Detection of Toxic and Biological Threats
Previous Article in Journal
Robust Forecasting for Energy Efficiency of Wireless Multimedia Sensor Networks
Previous Article in Special Issue
Ammonia Optical Sensing by Microring Resonators
Sensors 2007, 7(11), 2808-2824; doi:10.3390/s7112808

Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins

, , ,  and *
Center for Bio/Molecular Science & Engineering, Code 6900, Naval Research Laboratory, 4555 Overlook Ave. SW, Washington, DC 20375, USA
* Author to whom correspondence should be addressed.
Received: 24 October 2007 / Accepted: 14 November 2007 / Published: 16 November 2007
View Full-Text   |   Download PDF [459 KB, uploaded 21 June 2014]   |   Browse Figures


Many organisms secrete antimicrobial peptides (AMPs) for protection againstharmful microbes. The present study describes detection of botulinum neurotoxoids A, Band E using AMPs as recognition elements in an array biosensor. While AMP affinitieswere similar to those for anti-botulinum antibodies, differences in binding patterns wereobserved and can potentially be used for identification of toxoid serotype. Furthermore,some AMPs also demonstrated superior detection sensitivity compared to antibodies: toxoidA could be detected at 3.5 LD50 of the active toxin in a 75-min assay, whereas toxoids B andE were detected at 14 and 80 LD50 for their respective toxins.
Keywords: Antimicrobial peptide; botulinum toxin; detection; array; biosensor Antimicrobial peptide; botulinum toxin; detection; array; biosensor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Kulagina, N.V.; Anderson, G.P.; Ligler, F.S.; Shaffer, K.M.; Taitt, C.R. Antimicrobial Peptides: New Recognition Molecules for Detecting Botulinum Toxins. Sensors 2007, 7, 2808-2824.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert