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Sensors 2007, 7(11), 2510-2518; https://doi.org/10.3390/s7112510

Electrochemical Detection of a Dengue-related Oligonucleotide Sequence Using Ferrocenium as a Hybridization Indicator

1
Laboratório de Imunopatologia Keizo-Asami (LIKA), Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego, S/N, Campus Universitário, Cidade Universitária, Recife, PE – CEP: 50670-901, Brazil
2
IBB–Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001, Lisbon, Portugal
*
Author to whom correspondence should be addressed.
Received: 25 September 2007 / Accepted: 26 October 2007 / Published: 30 October 2007
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Abstract

A simple method for electrochemical detection of a synthetic 20-bpoligonucleotide sequence related with dengue virus genome was developed. Acomplimentary DNA probe sequence was electrostatically immobilized onto a glassycarbon electrode modified with chitosan. Electrochemical detection of hybridizationbetween probe and target was performed by cyclic voltammetry, using ferrocene (Fc ) as ahybridization label. After hybridization, the peak current response of Fc oxidationincreased around 26%. A higher voltammetric decay rate constant (kd) and a lower half-lifeperiod (t1/2) for the interaction of Fc with dsDNA compared to those with ssDNAquantitatively characterize the different strengths of interaction with both types of DNA.By combining the simplicity of DNA immobilization onto a chitosan film and suitablevoltammetric detection of hybridization concomitant with ferrocene attachment, a gooddiscrimination between ssDNA and dsDNA was obtained. View Full-Text
Keywords: modified glassy carbon electrode; DNA electrochemical biosensor; DNA immobilization; hybridization indicator; chitosan modified glassy carbon electrode; DNA electrochemical biosensor; DNA immobilization; hybridization indicator; chitosan
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Ribeiro Teles, F.R.; França dos Prazeres, D.M.; De Lima-Filho, J.L. Electrochemical Detection of a Dengue-related Oligonucleotide Sequence Using Ferrocenium as a Hybridization Indicator. Sensors 2007, 7, 2510-2518.

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