Next Article in Journal
Electrochemical Detection of a Dengue-related Oligonucleotide Sequence Using Ferrocenium as a Hybridization Indicator
Previous Article in Journal
Refractive Index Measurement within a Photonic Crystal Fibre Based on Short Wavelength Diffraction
Article Menu

Export Article

Open AccessArticle
Sensors 2007, 7(11), 2499-2509; doi:10.3390/s7112499

Formation and Fluorimetric Characterization of Micelles in a Micro-flow Through System with Static Micro Mixer

Institute for Micro and Nanotechnologies, Institute for Physics, Techn. Univ. Ilmenau, 98684 Ilmenau, Germany
*
Author to whom correspondence should be addressed.
Received: 9 October 2007 / Accepted: 22 October 2007 / Published: 30 October 2007
View Full-Text   |   Download PDF [232 KB, uploaded 21 June 2014]   |  

Abstract

The formation and behaviour of micelles of sodium dodecylsulfate in water byuse of a static micro mixer were studied. Trisbipyridylruthenium(II) was applied asindicator dye, 9-methylanthracene was used for fluorescence quenching. All experimentswere carried out by a micro fluid arrangement with three syringe pumps, a 2 1 two-stepstatic micro mixer (IPHT Jena) and a on-line micro fluorimetry including a luminescencediode for excitation, a blue glass filter (BG 7, Linos), two edge filters (RG 630, Linos) anda photo counting module (MP 900, Perkin Elmer). It was possible to measure thefluorescence inside the PTFE tube (inner diameter 0.5 mm) directly. A linear dependenceof fluorescence intensity from dye concentration was observed in absence of quencher andsurfactant as expected. An aggregation number of about 62 was found in the flow raterange between 300 and 800 μL/min. The fluorescence intensity increases slightly, butsignificant with increasing flow rate, if no quencher is present. In the presence of quencher,the fluorescence intensity decreases with decreasing surfactant concentration and withenhanced flow rate. The strength of the flow rate effect on the fluorescence increases withdecreasing surfactant concentration. The size of micelles was determined in micro channelsby the micro fluorimetric method in analogy to the conventional system. The micellesextract the quencher from the solution and lower, this way, the quenching effect. The sizeof micelles was estimated and it could be shown, that the flow rate has only low effect onthe aggregation number at the investigated flow rates. The effect of flow rate andsurfactant concentration on the fluorescence in the presence of quencher was interpreted asa shift in the micelle concentration due to the shear forces. It is expected, that thefluorescence intensity is lowered, if more quencher molecules are molecular disperse distributed inside the solution. Obviously, the lowered fluorescence intensity at higher flow rates suggests a reduction of the micelle density causing an increase of quencher concentration outside the micelles. View Full-Text
Keywords: microreaction technology; micelles; aggregation number; shear stress; laminar flow; fluorescence quenching microreaction technology; micelles; aggregation number; shear stress; laminar flow; fluorescence quenching
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Schuch, M.; Groß, G.A.; Köhler, J.M. Formation and Fluorimetric Characterization of Micelles in a Micro-flow Through System with Static Micro Mixer. Sensors 2007, 7, 2499-2509.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top