D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor

doi:10.3390/s7102484

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Sensors 2007, 7(10), 2484-2491; doi:10.3390/s7102484

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D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor

Viviana Scognamiglio ¹, Vincenzo Aurilia ¹, Nunzio Cennamo ², Paola Ringhieri ¹, Luisa Iozzino ¹, Micaela Tartaglia ¹, Maria Staiano ¹, Giuseppe Ruggiero ¹, Pierangelo Orlando ¹, Tullio Labella ¹, Luigi Zeni ², Annalisa Vitale ¹ and Sabato D’Auria ¹^,*

¹ Laboratory for Molecular Sensing, IBP, CNR, Via Pietro Castellino, 111 80131 Naples, Italy ² Department of Information Engineering, Seconda Università di Napoli, Italy

* Author to whom correspondence should be addressed.

Received: 3 October 2007 / Accepted: 21 October 2007 / Published: 24 October 2007

(This article belongs to the Special Issue Optical Biosensors)

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Abstract: D-Galactose/D-glucose-binding protein from E. coli (GGBP) is a monomer thatbinds glucose with high affinity. The protein structure of GGBP is organized in twoprincipal domains linked by a hinge region that form the sugar-binding site. In this workwe show that the mutant form of GGBP at the amino acid position 182 can be utilized as aprobe for the development of a non-consuming analyte fluorescence biosensor to monitorthe glucose level in diabetes health care.

Keywords: Biosensor; Optical sensing; Glucose; Diabetes; Fluorescence; Proteins

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Cite This Article

MDPI and ACS Style

Scognamiglio, V.; Aurilia, V.; Cennamo, N.; Ringhieri, P.; Iozzino, L.; Tartaglia, M.; Staiano, M.; Ruggiero, G.; Orlando, P.; Labella, T.; Zeni, L.; Vitale, A.; D’Auria, S. D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor. Sensors 2007, 7, 2484-2491.

AMA Style

Scognamiglio V., Aurilia V., Cennamo N., Ringhieri P., Iozzino L., Tartaglia M., Staiano M., Ruggiero G., Orlando P., Labella T., Zeni L., Vitale A., D’Auria S. D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor. Sensors. 2007; 7(10):2484-2491.

Chicago/Turabian Style

Scognamiglio, Viviana; Aurilia, Vincenzo; Cennamo, Nunzio; Ringhieri, Paola; Iozzino, Luisa; Tartaglia, Micaela; Staiano, Maria; Ruggiero, Giuseppe; Orlando, Pierangelo; Labella, Tullio; Zeni, Luigi; Vitale, Annalisa; D’Auria, Sabato. 2007. "D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor." Sensors 7, no. 10: 2484-2491.

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