Next Article in Journal
Refractive Index Measurement within a Photonic Crystal Fibre Based on Short Wavelength Diffraction
Next Article in Special Issue
Glucose Determination by Means of Steady-state and Time-course UV Fluorescence in Free or Immobilized Glucose Oxidase
Previous Article in Journal
Operational Mapping of Soil Moisture Using Synthetic Aperture Radar Data: Application to the Touch Basin (France)
Previous Article in Special Issue
Non-invasive Optical Biosensor for Probing Cell Signaling
Sensors 2007, 7(10), 2484-2491; doi:10.3390/s7102484
Article

D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor

1, 1, 2, 1, 1, 1, 1, 1, 1, 1, 2, 1 and 1,*
1 Laboratory for Molecular Sensing, IBP, CNR, Via Pietro Castellino, 111 80131 Naples, Italy 2 Department of Information Engineering, Seconda Università di Napoli, Italy
* Author to whom correspondence should be addressed.
Received: 3 October 2007 / Accepted: 21 October 2007 / Published: 24 October 2007
(This article belongs to the Special Issue Optical Biosensors)
View Full-Text   |   Download PDF [304 KB, uploaded 21 June 2014]   |   Browse Figures
SciFeed

Abstract

D-Galactose/D-glucose-binding protein from E. coli (GGBP) is a monomer thatbinds glucose with high affinity. The protein structure of GGBP is organized in twoprincipal domains linked by a hinge region that form the sugar-binding site. In this workwe show that the mutant form of GGBP at the amino acid position 182 can be utilized as aprobe for the development of a non-consuming analyte fluorescence biosensor to monitorthe glucose level in diabetes health care.
Keywords: Biosensor; Optical sensing; Glucose; Diabetes; Fluorescence; Proteins Biosensor; Optical sensing; Glucose; Diabetes; Fluorescence; Proteins
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Scognamiglio, V.; Aurilia, V.; Cennamo, N.; Ringhieri, P.; Iozzino, L.; Tartaglia, M.; Staiano, M.; Ruggiero, G.; Orlando, P.; Labella, T.; Zeni, L.; Vitale, A.; D’Auria, S. D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor. Sensors 2007, 7, 2484-2491.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert