Sensors 2007, 7(10), 2484-2491; doi:10.3390/s7102484
Article

D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor

Viviana Scognamiglio 1 , Vincenzo Aurilia 1 , Nunzio Cennamo 2 , Paola Ringhieri 1 , Luisa Iozzino 1 , Micaela Tartaglia 1 , Maria Staiano 1 , Giuseppe Ruggiero 1 , Pierangelo Orlando 1 , Tullio Labella 1 , Luigi Zeni 2 , Annalisa Vitale 1  and Sabato D’Auria 1,* email
1 Laboratory for Molecular Sensing, IBP, CNR, Via Pietro Castellino, 111 80131 Naples, Italy 2 Department of Information Engineering, Seconda Università di Napoli, Italy
* Author to whom correspondence should be addressed.
Received: 3 October 2007 / Accepted: 21 October 2007 / Published: 24 October 2007
(This article belongs to the Special Issue Optical Biosensors)
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Abstract: D-Galactose/D-glucose-binding protein from E. coli (GGBP) is a monomer thatbinds glucose with high affinity. The protein structure of GGBP is organized in twoprincipal domains linked by a hinge region that form the sugar-binding site. In this workwe show that the mutant form of GGBP at the amino acid position 182 can be utilized as aprobe for the development of a non-consuming analyte fluorescence biosensor to monitorthe glucose level in diabetes health care.
Keywords: Biosensor; Optical sensing; Glucose; Diabetes; Fluorescence; Proteins

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Cite This Article

MDPI and ACS Style

Scognamiglio, V.; Aurilia, V.; Cennamo, N.; Ringhieri, P.; Iozzino, L.; Tartaglia, M.; Staiano, M.; Ruggiero, G.; Orlando, P.; Labella, T.; Zeni, L.; Vitale, A.; D’Auria, S. D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor. Sensors 2007, 7, 2484-2491.

AMA Style

Scognamiglio V, Aurilia V, Cennamo N, Ringhieri P, Iozzino L, Tartaglia M, Staiano M, Ruggiero G, Orlando P, Labella T, Zeni L, Vitale A, D’Auria S. D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor. Sensors. 2007; 7(10):2484-2491.

Chicago/Turabian Style

Scognamiglio, Viviana; Aurilia, Vincenzo; Cennamo, Nunzio; Ringhieri, Paola; Iozzino, Luisa; Tartaglia, Micaela; Staiano, Maria; Ruggiero, Giuseppe; Orlando, Pierangelo; Labella, Tullio; Zeni, Luigi; Vitale, Annalisa; D’Auria, Sabato. 2007. "D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor." Sensors 7, no. 10: 2484-2491.

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