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Sensors 2007, 7(10), 2484-2491; doi:10.3390/s7102484
Article

D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor

1, 1, 2, 1, 1, 1, 1, 1, 1, 1, 2, 1 and 1,*
Received: 3 October 2007; Accepted: 21 October 2007 / Published: 24 October 2007
(This article belongs to the Special Issue Optical Biosensors)
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Abstract: D-Galactose/D-glucose-binding protein from E. coli (GGBP) is a monomer thatbinds glucose with high affinity. The protein structure of GGBP is organized in twoprincipal domains linked by a hinge region that form the sugar-binding site. In this workwe show that the mutant form of GGBP at the amino acid position 182 can be utilized as aprobe for the development of a non-consuming analyte fluorescence biosensor to monitorthe glucose level in diabetes health care.
Keywords: Biosensor; Optical sensing; Glucose; Diabetes; Fluorescence; Proteins Biosensor; Optical sensing; Glucose; Diabetes; Fluorescence; Proteins
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Scognamiglio, V.; Aurilia, V.; Cennamo, N.; Ringhieri, P.; Iozzino, L.; Tartaglia, M.; Staiano, M.; Ruggiero, G.; Orlando, P.; Labella, T.; Zeni, L.; Vitale, A.; D’Auria, S. D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor. Sensors 2007, 7, 2484-2491.

AMA Style

Scognamiglio V, Aurilia V, Cennamo N, Ringhieri P, Iozzino L, Tartaglia M, Staiano M, Ruggiero G, Orlando P, Labella T, Zeni L, Vitale A, D’Auria S. D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor. Sensors. 2007; 7(10):2484-2491.

Chicago/Turabian Style

Scognamiglio, Viviana; Aurilia, Vincenzo; Cennamo, Nunzio; Ringhieri, Paola; Iozzino, Luisa; Tartaglia, Micaela; Staiano, Maria; Ruggiero, Giuseppe; Orlando, Pierangelo; Labella, Tullio; Zeni, Luigi; Vitale, Annalisa; D’Auria, Sabato. 2007. "D-galactose/D-glucose-binding Protein from Escherichia coli as Probe for a Non-consuming Glucose Implantable Fluorescence Biosensor." Sensors 7, no. 10: 2484-2491.



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