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Fiber-Optic Biosensor Employing Alexa-Fluor Conjugated Antibody for Detection of Escherichia coli O157:H7 from Ground Beef in Four Hours
Sensors 2006, 6(8), 808-822; doi:10.3390/s6080808
Article

Antibody Immobilization on Waveguides Using aFlow–Through System Shows Improved Listeria monocytogenesDetection in an Automated Fiber Optic Biosensor: RAPTORTM

1, 2, 1,* , 3, 1, 1, 1, 1 and 1,*
1 Department of Food Science, Purdue University, 745 Agriculture Mall Dr., West Lafayette, IN, USA 47907 2 National Institute of Agricultural Engineering, RDA, 249 Seodun-dong, Suwon, Republic of Korea 441-100 3 Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, USA 47907
* Authors to whom correspondence should be addressed.
Received: 6 February 2006 / Accepted: 9 June 2006 / Published: 19 August 2006
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Abstract

Recent outbreaks of food borne illnesses continue to support the need for rapidand sensitive methods for detection of foodborne pathogens. A method for detecting Listeriamonocytogenes in food samples was developed using an automated fiber-optic-basedimmunosensor, RAPTORTM. Detection of L. monocytogenes in phosphate buffered saline(PBS) was performed to evaluate both static and flow through antibody immobilizationmethods for capture antibodies in a sandwich assay. Subsequent detection in frankfurtersamples was conducted using a flow through immobilization system. A two stage blockingusing biotinylated bovine serum albumin (b-BSA) and BSA was effectively employed toreduce the non-specific binding. The sandwich assay using static or flow through mode ofantibody immobilization could detect 1
Keywords: Biosensor; Listeria monocytogenes; fiber optic sensor; immunosensor; RAPTOR Biosensor; Listeria monocytogenes; fiber optic sensor; immunosensor; RAPTOR
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Nanduri, V.; Kim, G.; Morgan, M.T.; Ess, D.; Hahm, B.-K.; Kothapalli, A.; Valadez, A.; Geng, T.; Bhunia, A.K. Antibody Immobilization on Waveguides Using aFlow–Through System Shows Improved Listeria monocytogenesDetection in an Automated Fiber Optic Biosensor: RAPTORTM. Sensors 2006, 6, 808-822.

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