Next Article in Journal / Special Issue
Single-crystal Sapphire Based Optical Polarimetric Sensor for High Temperature Measurement
Previous Article in Journal / Special Issue
Fiber-Optic Biosensor Employing Alexa-Fluor Conjugated Antibody for Detection of Escherichia coli O157:H7 from Ground Beef in Four Hours
Sensors 2006, 6(8), 808-822; doi:10.3390/s6080808
Article

Antibody Immobilization on Waveguides Using aFlow–Through System Shows Improved Listeria monocytogenesDetection in an Automated Fiber Optic Biosensor: RAPTORTM

1, 2, 1,* , 3, 1, 1, 1, 1 and 1,*
Received: 6 February 2006; Accepted: 9 June 2006 / Published: 19 August 2006
View Full-Text   |   Download PDF [119 KB, uploaded 21 June 2014]   |   Browse Figures
Abstract: Recent outbreaks of food borne illnesses continue to support the need for rapidand sensitive methods for detection of foodborne pathogens. A method for detecting Listeriamonocytogenes in food samples was developed using an automated fiber-optic-basedimmunosensor, RAPTORTM. Detection of L. monocytogenes in phosphate buffered saline(PBS) was performed to evaluate both static and flow through antibody immobilizationmethods for capture antibodies in a sandwich assay. Subsequent detection in frankfurtersamples was conducted using a flow through immobilization system. A two stage blockingusing biotinylated bovine serum albumin (b-BSA) and BSA was effectively employed toreduce the non-specific binding. The sandwich assay using static or flow through mode ofantibody immobilization could detect 1
Keywords: Biosensor; Listeria monocytogenes; fiber optic sensor; immunosensor; RAPTOR Biosensor; Listeria monocytogenes; fiber optic sensor; immunosensor; RAPTOR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Nanduri, V.; Kim, G.; Morgan, M.T.; Ess, D.; Hahm, B.-K.; Kothapalli, A.; Valadez, A.; Geng, T.; Bhunia, A.K. Antibody Immobilization on Waveguides Using aFlow–Through System Shows Improved Listeria monocytogenesDetection in an Automated Fiber Optic Biosensor: RAPTORTM. Sensors 2006, 6, 808-822.

AMA Style

Nanduri V, Kim G, Morgan MT, Ess D, Hahm B-K, Kothapalli A, Valadez A, Geng T, Bhunia AK. Antibody Immobilization on Waveguides Using aFlow–Through System Shows Improved Listeria monocytogenesDetection in an Automated Fiber Optic Biosensor: RAPTORTM. Sensors. 2006; 6(8):808-822.

Chicago/Turabian Style

Nanduri, Viswaprakash; Kim, Giyoung; Morgan, Mark T.; Ess, Daniel; Hahm, Byoung-Kwon; Kothapalli, Aparna; Valadez, Angela; Geng, Tao; Bhunia, Arun K. 2006. "Antibody Immobilization on Waveguides Using aFlow–Through System Shows Improved Listeria monocytogenesDetection in an Automated Fiber Optic Biosensor: RAPTORTM." Sensors 6, no. 8: 808-822.


Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert