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Sensors 2006, 6(8), 808-822; doi:10.3390/s6080808
Article

Antibody Immobilization on Waveguides Using aFlow–Through System Shows Improved Listeria monocytogenesDetection in an Automated Fiber Optic Biosensor: RAPTORTM

1, 2, 1,* , 3, 1, 1, 1, 1 and 1,*
1 Department of Food Science, Purdue University, 745 Agriculture Mall Dr., West Lafayette, IN, USA 47907 2 National Institute of Agricultural Engineering, RDA, 249 Seodun-dong, Suwon, Republic of Korea 441-100 3 Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, USA 47907
* Authors to whom correspondence should be addressed.
Received: 6 February 2006 / Accepted: 9 June 2006 / Published: 19 August 2006
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Abstract

Recent outbreaks of food borne illnesses continue to support the need for rapidand sensitive methods for detection of foodborne pathogens. A method for detecting Listeriamonocytogenes in food samples was developed using an automated fiber-optic-basedimmunosensor, RAPTORTM. Detection of L. monocytogenes in phosphate buffered saline(PBS) was performed to evaluate both static and flow through antibody immobilizationmethods for capture antibodies in a sandwich assay. Subsequent detection in frankfurtersamples was conducted using a flow through immobilization system. A two stage blockingusing biotinylated bovine serum albumin (b-BSA) and BSA was effectively employed toreduce the non-specific binding. The sandwich assay using static or flow through mode ofantibody immobilization could detect 1
Keywords: Biosensor; Listeria monocytogenes; fiber optic sensor; immunosensor; RAPTOR Biosensor; Listeria monocytogenes; fiber optic sensor; immunosensor; RAPTOR
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Nanduri, V.; Kim, G.; Morgan, M.T.; Ess, D.; Hahm, B.-K.; Kothapalli, A.; Valadez, A.; Geng, T.; Bhunia, A.K. Antibody Immobilization on Waveguides Using aFlow–Through System Shows Improved Listeria monocytogenesDetection in an Automated Fiber Optic Biosensor: RAPTORTM. Sensors 2006, 6, 808-822.

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