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• Nanduri, V
• Kim, G
• Morgan, MT.
• Ess, D
• Hahm, B
• Kothapalli, A
• Valadez, A
• Geng, T
• Bhunia, AK.
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• Nanduri, V
• Kim, G
• Morgan, MT.
• Ess, D
• Hahm, B
• Kothapalli, A
• Valadez, A
• Geng, T
• Bhunia, AK.
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• Nanduri, V
• Kim, G
• Morgan, MT.
• Ess, D
• Hahm, B
• Kothapalli, A
• Valadez, A
• Geng, T
• Bhunia, AK.

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Sensors 2006, 6(8), 808-822; doi:10.3390/s6080808

Article

Antibody Immobilization on Waveguides Using aFlow–Through System Shows Improved Listeria monocytogenesDetection in an Automated Fiber Optic Biosensor: RAPTOR^TM

Viswaprakash Nanduri ¹ , Giyoung Kim ² , Mark T. Morgan ^1,* , Daniel Ess ³ , Byoung-Kwon Hahm ¹ , Aparna Kothapalli ¹ , Angela Valadez ¹ , Tao Geng ¹  and Arun K. Bhunia ^1,*

¹ Department of Food Science, Purdue University, 745 Agriculture Mall Dr., West Lafayette, IN, USA 47907 ² National Institute of Agricultural Engineering, RDA, 249 Seodun-dong, Suwon, Republic of Korea 441-100 ³ Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN, USA 47907

* Authors to whom correspondence should be addressed.

Received: 6 February 2006 / Accepted: 9 June 2006 / Published: 19 August 2006

(This article belongs to the Special Issue Photonic Sensors for Chemical, Biological, and Nuclear Agent Detection)

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Abstract: Recent outbreaks of food borne illnesses continue to support the need for rapidand sensitive methods for detection of foodborne pathogens. A method for detecting Listeriamonocytogenes in food samples was developed using an automated fiber-optic-basedimmunosensor, RAPTOR^TM. Detection of L. monocytogenes in phosphate buffered saline(PBS) was performed to evaluate both static and flow through antibody immobilizationmethods for capture antibodies in a sandwich assay. Subsequent detection in frankfurtersamples was conducted using a flow through immobilization system. A two stage blockingusing biotinylated bovine serum albumin (b-BSA) and BSA was effectively employed toreduce the non-specific binding. The sandwich assay using static or flow through mode ofantibody immobilization could detect 1

Keywords: Biosensor; Listeria monocytogenes; fiber optic sensor; immunosensor; RAPTOR

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