Sensors 2006, 6(8), 785-795; doi:10.3390/s6080785
Article

Aptamer Based Microsphere Biosensor for Thrombin Detection

Department of Biological Engineering, 240D Life Sciences Center, 1201 East Rollins Street, University of Missouri—Columbia Columbia, Missouri 65211, USA
* Author to whom correspondence should be addressed.
Received: 17 January 2006; Accepted: 12 April 2006 / Published: 16 August 2006
PDF Full-text Download PDF Full-Text [191 KB, uploaded 20 June 2008 16:51 CEST]
Abstract: We have developed an optical microsphere resonator biosensor using aptamer asreceptor for the measurement of the important biomolecule thrombin. The sphere surface ismodified with anti-thrombin aptamer, which has excellent binding affinity and selectivityfor thrombin. Binding of the thrombin at the sphere surface is monitored by the spectralposition of the microsphere’s whispering gallery mode resonances. A detection limit on theorder of 1 NIH Unit/mL is demonstrated. Control experiments with non-aptameroligonucleotide and BSA are also carried out to confirm the specific binding betweenaptamer and thrombin. We expect that this demonstration will lead to the development ofhighly sensitive biomarker sensors based on aptamer with lower cost and higher throughputthan current technology.
Keywords: Microsphere; ring resonator; whispering gallery mode; aptamer; thrombin

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Zhu, H.; Suter, J.D.; White, I.M.; Fan, X. Aptamer Based Microsphere Biosensor for Thrombin Detection. Sensors 2006, 6, 785-795.

AMA Style

Zhu H, Suter JD, White IM, Fan X. Aptamer Based Microsphere Biosensor for Thrombin Detection. Sensors. 2006; 6(8):785-795.

Chicago/Turabian Style

Zhu, Hongying; Suter, Jonathan D.; White, Ian M.; Fan, Xudong. 2006. "Aptamer Based Microsphere Biosensor for Thrombin Detection." Sensors 6, no. 8: 785-795.

Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert