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Special Issue of Photonic Sensors for Chemical, Biological, and Physical Parameter Detection
Sensors 2006, 6(8), 785-795; doi:10.3390/s6080785
Article

Aptamer Based Microsphere Biosensor for Thrombin Detection

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Received: 17 January 2006; Accepted: 12 April 2006 / Published: 16 August 2006
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Abstract: We have developed an optical microsphere resonator biosensor using aptamer asreceptor for the measurement of the important biomolecule thrombin. The sphere surface ismodified with anti-thrombin aptamer, which has excellent binding affinity and selectivityfor thrombin. Binding of the thrombin at the sphere surface is monitored by the spectralposition of the microsphere’s whispering gallery mode resonances. A detection limit on theorder of 1 NIH Unit/mL is demonstrated. Control experiments with non-aptameroligonucleotide and BSA are also carried out to confirm the specific binding betweenaptamer and thrombin. We expect that this demonstration will lead to the development ofhighly sensitive biomarker sensors based on aptamer with lower cost and higher throughputthan current technology.
Keywords: Microsphere; ring resonator; whispering gallery mode; aptamer; thrombin Microsphere; ring resonator; whispering gallery mode; aptamer; thrombin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Zhu, H.; Suter, J.D.; White, I.M.; Fan, X. Aptamer Based Microsphere Biosensor for Thrombin Detection. Sensors 2006, 6, 785-795.

AMA Style

Zhu H, Suter JD, White IM, Fan X. Aptamer Based Microsphere Biosensor for Thrombin Detection. Sensors. 2006; 6(8):785-795.

Chicago/Turabian Style

Zhu, Hongying; Suter, Jonathan D.; White, Ian M.; Fan, Xudong. 2006. "Aptamer Based Microsphere Biosensor for Thrombin Detection." Sensors 6, no. 8: 785-795.


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