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• Zhu, H
• Suter, JD.
• White, IM.
• Fan, X
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• Zhu, H
• Suter, JD.
• White, IM.
• Fan, X
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• Zhu, H
• Suter, JD.
• White, IM.
• Fan, X

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Sensors 2006, 6(8), 785-795; doi:10.3390/s6080785

Article

Aptamer Based Microsphere Biosensor for Thrombin Detection

Hongying Zhu, Jonathan D. Suter, Ian M. White and Xudong Fan*

Department of Biological Engineering, 240D Life Sciences Center, 1201 East Rollins Street, University of Missouri—Columbia Columbia, Missouri 65211, USA

* Author to whom correspondence should be addressed.

Received: 17 January 2006 / Accepted: 12 April 2006 / Published: 16 August 2006

(This article belongs to the Special Issue Photonic Sensors for Chemical, Biological, and Nuclear Agent Detection)

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Abstract: We have developed an optical microsphere resonator biosensor using aptamer asreceptor for the measurement of the important biomolecule thrombin. The sphere surface ismodified with anti-thrombin aptamer, which has excellent binding affinity and selectivityfor thrombin. Binding of the thrombin at the sphere surface is monitored by the spectralposition of the microsphere’s whispering gallery mode resonances. A detection limit on theorder of 1 NIH Unit/mL is demonstrated. Control experiments with non-aptameroligonucleotide and BSA are also carried out to confirm the specific binding betweenaptamer and thrombin. We expect that this demonstration will lead to the development ofhighly sensitive biomarker sensors based on aptamer with lower cost and higher throughputthan current technology.

Keywords: Microsphere; ring resonator; whispering gallery mode; aptamer; thrombin

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