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Sensors 2006, 6(4), 428-434; doi:10.3390/s6040428

pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods

Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Ks. Trojdena 4, 02-109 Warsaw, Poland
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Received: 1 September 2005 / Accepted: 4 January 2006 / Published: 7 April 2006
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Abstract

In this paper, methods of sample preparation for potentiometric measurement ofphenylalanine are presented. Basing on the spectrophotometric measurements ofphenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe,0,4 mM NAD+ , 2U L-PheDH) were determined. Then, the absorption spectrum of thereaction product, NADH, was monitored (maximum peak at 340 nm). The results obtainedby the spectrophotometric method were compared with the results obtained by thecolourimetry, using pH indicators. The above-mentioned two methods will be used asreferences for potentiometric measurements of phenylalanine concentration. View Full-Text
Keywords: Phenylalanine determination; enzymatic methods; pH-based biosensing. Phenylalanine determination; enzymatic methods; pH-based biosensing.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Pijanowska, D.G.; Remiszewska, E. pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods. Sensors 2006, 6, 428-434.

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