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Sensors 2006, 6(4), 428-434; doi:10.3390/s6040428
Article
pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods
Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Ks. Trojdena 4, 02-109 Warsaw, Poland
* Author to whom correspondence should be addressed.
Received: 1 September 2005 / Accepted: 4 January 2006 / Published: 7 April 2006
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Abstract: In this paper, methods of sample preparation for potentiometric measurement ofphenylalanine are presented. Basing on the spectrophotometric measurements ofphenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe,0,4 mM NAD+ , 2U L-PheDH) were determined. Then, the absorption spectrum of thereaction product, NADH, was monitored (maximum peak at 340 nm). The results obtainedby the spectrophotometric method were compared with the results obtained by thecolourimetry, using pH indicators. The above-mentioned two methods will be used asreferences for potentiometric measurements of phenylalanine concentration.
Keywords: Phenylalanine determination; enzymatic methods; pH-based biosensing.
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MDPI and ACS Style
Pijanowska, D.G.; Remiszewska, E. pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods. Sensors 2006, 6, 428-434.
AMA StylePijanowska D.G., Remiszewska E. pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods. Sensors. 2006; 6(4):428-434.
Chicago/Turabian StylePijanowska, Dorota G.; Remiszewska, Elżbieta. 2006. "pH-based Detection of Phenylalnine by Potentiometric and Colorimetric Methods." Sensors 6, no. 4: 428-434.
Sensors
EISSN 1424-8220
Published by MDPI Publishing, Basel, Switzerland
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