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Sensors 2006, 6(4), 370-379; doi:10.3390/s6040370

Surface Modification for Microreactor Fabrication

1
Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, ul. Ks. Trojdena 4, 02-109 Warsaw, Poland
2
Centro Per La Ricerca Scientifica E Tecnologia, ITCirst, Via Sommarive 18, Povo, 38050 Trento, Italy
*
Author to whom correspondence should be addressed.
Received: 1 September 2005 / Accepted: 4 January 2006 / Published: 7 April 2006
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Abstract

In this paper, methods of surface modification of different supports, i.e. glass andpolymeric beads for enzyme immobilisation are described. The developed method ofenzyme immobilisation is based on Schiff’s base formation between the amino groups onthe enzyme surface and the aldehyde groups on the chemically modified surface of thesupports. The surface of silicon modified by APTS and GOPS with immobilised enzymewas characterised by atomic force microscopy (AFM), time-of-flight secondary ion massspectroscopy (ToF-SIMS) and infrared spectroscopy (FTIR). The supports withimmobilised enzyme (urease) were also tested in combination with microreactors fabricatedin silicon and Perspex, operating in a flow-through system. For microreactors filled withurease immobilised on glass beads (Sigma) and on polymeric beads (PAN), a very high andstable signal (pH change) was obtained. The developed method of urease immobilisationcan be stated to be very effective. View Full-Text
Keywords: Microreactors; surface modification; enzyme immobilisation; lab-on-a-chip Microreactors; surface modification; enzyme immobilisation; lab-on-a-chip
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Pijanowska, D.G.; Remiszewska, E.; Pederzolli, C.; Lunelli, L.; Vendano, M.; Canteri, R.; Dudziński, K.; Kruk, J.; Torbicz, W. Surface Modification for Microreactor Fabrication. Sensors 2006, 6, 370-379.

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