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Modelling of Amperometric Biosensors in the Case of Substrate Inhibition
Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Sauletekio al. 11, 10223 Vilnius, Lithuania
Faculty of Mathematics and Informatics, Vilnius University, Naugarduko 24, LT−03225 Vilnius, Lithuania
* Author to whom correspondence should be addressed.
Received: 15 September 2006; Accepted: 6 November 2006 / Published: 7 November 2006
Abstract: The response of an amperometric biosensor at mixed enzyme kinetics anddiffusion limitations was modelled digitally in the case of substrate inhibition. Digitalsimulations were carried out using a finite difference technique. Calculations showedcomplex kinetics of biosensor response. At low enzyme activity and substrate concentration(S0), the response of the sensor looks like it is limited by a simple substrate diffusion. Atsubstrate concentration comparable to the Michaelis-Menten constant (KM), the responsechange shows a maximal value. A sharp response change was indicated at high enzymeactivity and high (4.9 > S0/KM > 4.5) substrate concentration. This was explained by multiconcentrationof substrate generation inside the enzyme layer. This conclusion wasconfirmed by the analytical solution of the simplified biosensor model with externaldiffusion limitation at steady-state conditions. The complex kinetics of response changeproduces different calibration graphs for biosensor response at transition and steady state.
Keywords: biosensor; enzyme inhibition; diffusion; modelling
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MDPI and ACS Style
Kulys, J.; Baronas, R. Modelling of Amperometric Biosensors in the Case of Substrate Inhibition. Sensors 2006, 6, 1513-1522.
Kulys J, Baronas R. Modelling of Amperometric Biosensors in the Case of Substrate Inhibition. Sensors. 2006; 6(11):1513-1522.
Kulys, Juozas; Baronas, Romas. 2006. "Modelling of Amperometric Biosensors in the Case of Substrate Inhibition." Sensors 6, no. 11: 1513-1522.