Sensors 2006, 6(11), 1513-1522; doi:10.3390/s6111513

Modelling of Amperometric Biosensors in the Case of Substrate Inhibition

1 Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Sauletekio al. 11, 10223 Vilnius, Lithuania 2 Faculty of Mathematics and Informatics, Vilnius University, Naugarduko 24, LT−03225 Vilnius, Lithuania
* Author to whom correspondence should be addressed.
Received: 15 September 2006; Accepted: 6 November 2006 / Published: 7 November 2006
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Abstract: The response of an amperometric biosensor at mixed enzyme kinetics anddiffusion limitations was modelled digitally in the case of substrate inhibition. Digitalsimulations were carried out using a finite difference technique. Calculations showedcomplex kinetics of biosensor response. At low enzyme activity and substrate concentration(S0), the response of the sensor looks like it is limited by a simple substrate diffusion. Atsubstrate concentration comparable to the Michaelis-Menten constant (KM), the responsechange shows a maximal value. A sharp response change was indicated at high enzymeactivity and high (4.9 > S0/KM > 4.5) substrate concentration. This was explained by multiconcentrationof substrate generation inside the enzyme layer. This conclusion wasconfirmed by the analytical solution of the simplified biosensor model with externaldiffusion limitation at steady-state conditions. The complex kinetics of response changeproduces different calibration graphs for biosensor response at transition and steady state.
Keywords: biosensor; enzyme inhibition; diffusion; modelling

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MDPI and ACS Style

Kulys, J.; Baronas, R. Modelling of Amperometric Biosensors in the Case of Substrate Inhibition. Sensors 2006, 6, 1513-1522.

AMA Style

Kulys J, Baronas R. Modelling of Amperometric Biosensors in the Case of Substrate Inhibition. Sensors. 2006; 6(11):1513-1522.

Chicago/Turabian Style

Kulys, Juozas; Baronas, Romas. 2006. "Modelling of Amperometric Biosensors in the Case of Substrate Inhibition." Sensors 6, no. 11: 1513-1522.

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