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• Li, N
• Wei, Y
• Zhu, J
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• Li, N
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Sensors 2005, 5(4), 277-283; doi:10.3390/s5040277

Article

A H₂O₂ Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix

Hui Yao, Nan Li, Yan-Li Wei and Jun-Jie Zhu*

Key Lab. of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, P.R. China

* Author to whom correspondence should be addressed.

Received: 8 June 2004 / Accepted: 14 November 2004 / Published: 4 May 2005

(This article belongs to the Special Issue Papers presented at I3S2004, Nanjing)

Download PDF Full-Text [235 KB, uploaded 20 June 2008 16:51 CEST]

Abstract: A simple and promising H₂O₂ biosensor has been developed by successfulentrapment of horseradish peroxidase (HRP) in a gelatine matrix which was cross-linkedwith formaldehyde. The large microscopic surface area and porous morphology of thegelatine matrix lead to high enzyme loading and the enzyme entrapped in this matrix canretain its bioactivity. This biosensor exhibited a fast amperometric response to hydrogenperoxide (H₂O₂). The linear range for H₂O₂ determination was from 2.5×10^-5 to2.5×10^-3 M, with a detection limit of 2.0×10^-6 M based on S / N = 3. This biosensorpossessed very good reproducibility.

Keywords: H2O2 biosensor; horseradish peroxidase; gelatine matrix.

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