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A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide
Sensors 2005, 5(4), 277-283; doi:10.3390/s5040277
Article

A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix

, ,  and *
Received: 8 June 2004; Accepted: 14 November 2004 / Published: 4 May 2005
(This article belongs to the Special Issue Papers presented at I3S2004, Nanjing)
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Abstract: A simple and promising H2O2 biosensor has been developed by successfulentrapment of horseradish peroxidase (HRP) in a gelatine matrix which was cross-linkedwith formaldehyde. The large microscopic surface area and porous morphology of thegelatine matrix lead to high enzyme loading and the enzyme entrapped in this matrix canretain its bioactivity. This biosensor exhibited a fast amperometric response to hydrogenperoxide (H2O2). The linear range for H2O2 determination was from 2.5×10-5 to2.5×10-3 M, with a detection limit of 2.0×10-6 M based on S / N = 3. This biosensorpossessed very good reproducibility.
Keywords: H2O2 biosensor; horseradish peroxidase; gelatine matrix. H2O2 biosensor; horseradish peroxidase; gelatine matrix.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yao, H.; Li, N.; Wei, Y.-L.; Zhu, J.-J. A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix. Sensors 2005, 5, 277-283.

AMA Style

Yao H, Li N, Wei Y-L, Zhu J-J. A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix. Sensors. 2005; 5(4):277-283.

Chicago/Turabian Style

Yao, Hui; Li, Nan; Wei, Yan-Li; Zhu, Jun-Jie. 2005. "A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix." Sensors 5, no. 4: 277-283.


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