Next Article in Journal / Special Issue
A H2O2 Biosensor Based on Immobilization of HorseradishPeroxidase in a Gelatine Network Matrix
Previous Article in Journal / Special Issue
Bio-nanocomposite Photoelectrode Composed of the BacteriaPhotosynthetic Reaction Center Entrapped on a NanocrystallineTiO2 Matrix
Sensors 2005, 5(4), 266-276; doi:10.3390/s5040266
Article

A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide

* ,  and
Received: 25 June 2004; Accepted: 14 November 2004 / Published: 4 May 2005
(This article belongs to the Special Issue Papers presented at I3S2004, Nanjing)
View Full-Text   |   Download PDF [166 KB, uploaded 20 June 2014]   |   Browse Figures
Abstract: An amperometric biosensor for hydrogen peroxide (H2O2) was developed viaan easy and effective enzyme immobilization method with the “sandwich” configuration:ferrocene-chitosan: HRP: chitosan-glyoxal using a glassy carbon electrode as the basicelectrode. In order to prevent the loss of immobilized HRP under optimized conditions,the biosensor surface was cross-linked with glyoxal. Ferrocene was selected andimmobilized on the glassy carbon electrode surface as a mediator. The fabricationprocedure was systematically optimized to improve the biosensor performance. Thebiosensor had a fast response of less than 10 s to H2O2, with a linear range of 3.5×10-5 to1.1×10-3 M, and a detection limit of 8.0×10-6 M based on S/N = 3.
Keywords: horseradish peroxidase; biosensor; hydrogen peroxide; chitosan horseradish peroxidase; biosensor; hydrogen peroxide; chitosan
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Wang, H.-S.; Pan, Q.-X.; Wang, G.-X. A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide. Sensors 2005, 5, 266-276.

AMA Style

Wang H-S, Pan Q-X, Wang G-X. A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide. Sensors. 2005; 5(4):266-276.

Chicago/Turabian Style

Wang, Huai-Sheng; Pan, Qian-Xiu; Wang, Gui-Xiang. 2005. "A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide." Sensors 5, no. 4: 266-276.


Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert