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Sensors 2005, 5(4), 266-276; doi:10.3390/s5040266
Article

A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide

* ,  and
Department of Chemistry, Institute of Analytical Chemistry, Liaocheng University, Liaocheng, 252059, China
* Author to whom correspondence should be addressed.
Received: 25 June 2004 / Accepted: 14 November 2004 / Published: 4 May 2005
(This article belongs to the Special Issue Papers presented at I3S2004, Nanjing)
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Abstract

An amperometric biosensor for hydrogen peroxide (H2O2) was developed viaan easy and effective enzyme immobilization method with the “sandwich” configuration:ferrocene-chitosan: HRP: chitosan-glyoxal using a glassy carbon electrode as the basicelectrode. In order to prevent the loss of immobilized HRP under optimized conditions,the biosensor surface was cross-linked with glyoxal. Ferrocene was selected andimmobilized on the glassy carbon electrode surface as a mediator. The fabricationprocedure was systematically optimized to improve the biosensor performance. Thebiosensor had a fast response of less than 10 s to H2O2, with a linear range of 3.5×10-5 to1.1×10-3 M, and a detection limit of 8.0×10-6 M based on S/N = 3.
Keywords: horseradish peroxidase; biosensor; hydrogen peroxide; chitosan horseradish peroxidase; biosensor; hydrogen peroxide; chitosan
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Wang, H.-S.; Pan, Q.-X.; Wang, G.-X. A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide. Sensors 2005, 5, 266-276.

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