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Sensors 2005, 5(4), 171-184; doi:10.3390/s5040171
Article

A Novel Supramolecular Assembly Film of Porphyrin Bound DNA: Characterization and Catalytic Behaviors Towards Nitric Oxide

1, 2 and 1,*
1 Department of Chemistry, Faculty of Science, Kanazawa University, Natural Science Hall 1, Kakuma, Kanazawa, Ishikawa 920-1192, Japan 2 State Key Laboratory of Coordination Chemistry, Department of Chemistry, Institute of Chemical Biology, Nanjing University, Nanjing 210093, PR China
* Author to whom correspondence should be addressed.
Received: 28 May 2004 / Accepted: 29 September 2004 / Published: 26 April 2005
(This article belongs to the Special Issue Papers presented at I3S2004, Nanjing)
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Abstract

A stable Fe(4-TMPyP)-DNA-PADDA (FePyDP) film was characterized onpyrolytic graphite electrode (PGE) or an indium-tin oxide (ITO) electrode through thesupramolecular interaction between water-soluble iron porphyrin (Fe(4-TMPyP)) and DNAtemplate, where PADDA (poly(acrylamide-co-diallyldimethylammonium chloride) isemployed as a co-immobilizing polymer. Cyclic voltammetry of FePyDP film showed a pairof reversible FeIII/FeII redox peaks and an irreversible FeIV/FeIII peak at –0.13 V and 0.89vs. Ag|AgCl in pH 7.4 PBS, respectively. An excellent catalytic reduction of NO wasdisplayed at –0.61 V vs. Ag|AgCl at a FePyDP film modified electrode.Chronoamperometric experiments demonstrated a rapid response to the reduction of NOwith a linear range from 0.1 to 90 μM and a detection limit of 30 nM at a signal-to-noiseratio of 3. On the other hand, it is the first time to apply high-valent iron porphyrin ascatalyst at modified electrode for NO catalytic oxidation at 0.89 vs. Ag|AgCl. The sensorshows a high selectivity of some endogenous electroactive substances in biological systems.The mechanism of response of the sensors to NO is preliminary studied.
Keywords: nitric oxide determination; supramolecular assembly; porphyrin bound DNA; Elechtrochemical sensor nitric oxide determination; supramolecular assembly; porphyrin bound DNA; Elechtrochemical sensor
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Lei, J.; Ju, H.; Ikeda, O. A Novel Supramolecular Assembly Film of Porphyrin Bound DNA: Characterization and Catalytic Behaviors Towards Nitric Oxide. Sensors 2005, 5, 171-184.

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