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Sensors 2005, 5(4), 171-184; doi:10.3390/s5040171
Article

A Novel Supramolecular Assembly Film of Porphyrin Bound DNA: Characterization and Catalytic Behaviors Towards Nitric Oxide

1, 2 and 1,*
Received: 28 May 2004; Accepted: 29 September 2004 / Published: 26 April 2005
(This article belongs to the Special Issue Papers presented at I3S2004, Nanjing)
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Abstract: A stable Fe(4-TMPyP)-DNA-PADDA (FePyDP) film was characterized onpyrolytic graphite electrode (PGE) or an indium-tin oxide (ITO) electrode through thesupramolecular interaction between water-soluble iron porphyrin (Fe(4-TMPyP)) and DNAtemplate, where PADDA (poly(acrylamide-co-diallyldimethylammonium chloride) isemployed as a co-immobilizing polymer. Cyclic voltammetry of FePyDP film showed a pairof reversible FeIII/FeII redox peaks and an irreversible FeIV/FeIII peak at –0.13 V and 0.89vs. Ag|AgCl in pH 7.4 PBS, respectively. An excellent catalytic reduction of NO wasdisplayed at –0.61 V vs. Ag|AgCl at a FePyDP film modified electrode.Chronoamperometric experiments demonstrated a rapid response to the reduction of NOwith a linear range from 0.1 to 90 μM and a detection limit of 30 nM at a signal-to-noiseratio of 3. On the other hand, it is the first time to apply high-valent iron porphyrin ascatalyst at modified electrode for NO catalytic oxidation at 0.89 vs. Ag|AgCl. The sensorshows a high selectivity of some endogenous electroactive substances in biological systems.The mechanism of response of the sensors to NO is preliminary studied.
Keywords: nitric oxide determination; supramolecular assembly; porphyrin bound DNA; Elechtrochemical sensor nitric oxide determination; supramolecular assembly; porphyrin bound DNA; Elechtrochemical sensor
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lei, J.; Ju, H.; Ikeda, O. A Novel Supramolecular Assembly Film of Porphyrin Bound DNA: Characterization and Catalytic Behaviors Towards Nitric Oxide. Sensors 2005, 5, 171-184.

AMA Style

Lei J, Ju H, Ikeda O. A Novel Supramolecular Assembly Film of Porphyrin Bound DNA: Characterization and Catalytic Behaviors Towards Nitric Oxide. Sensors. 2005; 5(4):171-184.

Chicago/Turabian Style

Lei, Jianping; Ju, Huangxian; Ikeda, Osamu. 2005. "A Novel Supramolecular Assembly Film of Porphyrin Bound DNA: Characterization and Catalytic Behaviors Towards Nitric Oxide." Sensors 5, no. 4: 171-184.


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