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Sensors 2005, 5(12), 537-545; doi:10.3390/s5120537

Amperometric Detection of Nitric Oxide with Microsensor in the Medium of Seawater and Its Applications

Institute of Marine Chemistry, Ocean University of China, Qingdao 266003, China
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Received: 8 June 2005 / Accepted: 10 December 2005 / Published: 15 December 2005
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Abstract

Amperometric detection of nitric oxide with ISO-NOPMC NO microsensors(WPI) is systemically studied in the six media including seawater. The linear range of themicrosensor for NO was from 10-6 to 10-9 mol/l and the detection limit was4.2×10-10 mol/L(S/N=2). With this method, we provide preliminary evidence that NOproduction could be a general attribute of marine alga (Haeterosigma akashiwo).Experiment conducted with inhibitor of uncoupler 2,4-DNP (2,4-dinitropheno) revealed thatNR (nitrate reductase) activity is responsible for NO formation.
Keywords: Amperometric detection; ISO-NOPMC NO microsensors; Nitric oxide; Seawater; Haeterosigma akashiwo Amperometric detection; ISO-NOPMC NO microsensors; Nitric oxide; Seawater; Haeterosigma akashiwo
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Xing, L.; Zhang, Z.-B.; Liu, C.-Y.; Wu, Z.-Z.; Lin, C. Amperometric Detection of Nitric Oxide with Microsensor in the Medium of Seawater and Its Applications. Sensors 2005, 5, 537-545.

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