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Sensors 2004, 4(5), 71-83; doi:10.3390/s40500071

Supermolecular Interaction of Ferrocenium with Yeast DNA and Application in Electrochemical Sensing for Hybridization Recognition of Yeast DNA

Department of Chemistry, State Key Laboratory of Coordination Chemistry, Institute of Chemical Biology, Nanjing University, Nanjing 210093, China
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Received: 30 April 2004 / Accepted: 30 May 2004 / Published: 20 July 2004
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Abstract

The supermolecular interactions of single-stranded yeast DNA (ssDNA) and double-stranded DNA (dsDNA) with ferrocenium (Fc+) both in solution and at the electrode/solution interface were studied with UV spectroscopy and electrochemical method. The interaction of covalently immobilized dsDNA with Fc+ resulted in a change of the electrode behavior from diffusion-controlled to surface-controlled and an increase in the current response of Fc+ reduction, which has been used to develop a novel electrochemical yeast DNA sensor for hybridization recognition of immobilized yeast ssDNA to its complementary ssDNA (cDNA). The adsorption constants of Fc+ on ssDNA and dsDNA modified gold electrode surface were (3.38±0.04)×103 M-1 and (2.02±0.02)×104 M-1, respectively, indicating a higher affinity of dsDNA to Fc+. UV spectra and the influence of ion strength showed that the interaction mode between Fc+ and dsDNA in solution might be a groove binding. The interaction between Fc+ and immobilized DNA was mainly an electrostatic model. View Full-Text
Keywords: DNA Biosensors; supermolecular interaction; yeast DNA; ferrocenium; hybridization recognition DNA Biosensors; supermolecular interaction; yeast DNA; ferrocenium; hybridization recognition
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Ju, H.; Ye, B.; Gu, J. Supermolecular Interaction of Ferrocenium with Yeast DNA and Application in Electrochemical Sensing for Hybridization Recognition of Yeast DNA. Sensors 2004, 4, 71-83.

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