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Sensors 2003, 3(7), 248-262; doi:10.3390/s30700248
Article

The Influence of the Enzyme Membrane Thickness on the Response of Amperometric Biosensors

1,* , 1,2 and 3
1 Faculty of Mathematics and Informatics, Vilnius University, Naugarduko 24, 2600 Vilnius, Lithuania 2 Institute of Mathematics and Informatics, Akademijos 4, 2600 Vilnius, Lithuania 3 Institute of Biochemistry, Mokslininku 12, 2600 Vilnius, Lithuania
* Author to whom correspondence should be addressed.
Received: 22 May 2003 / Accepted: 10 July 2003 / Published: 27 July 2003
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Abstract

A mathematical model of amperometric biosensors has been developed. The model is based on non-stationary diffusion equations containing a non-linear term related to Michaelis-Menten kinetics of the enzymatic reaction. Using digital simulation, the influence of the thickness of enzyme membrane on the biosensor response was investigated. The digital simulation of the biosensor operation showed the non-monotonous change of the maximal biosensor current versus the membrane thickness at the various maximal enzymatic rates. Digital simulation was carried out using the finite difference technique. Results of the numerical simulation was compared with known analytical solutions. This paper presents a framework for selection of the membrane thickness, ensuring the sufficiently stable sensitivity of a biosensor in a required range of the maximal enzymatic rate.
Keywords: Amperometric biosensor; enzyme membrane; diffusion; modelling; simulation Amperometric biosensor; enzyme membrane; diffusion; modelling; simulation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Baronas, R.; Ivanauskas, F.; Kulys, J. The Influence of the Enzyme Membrane Thickness on the Response of Amperometric Biosensors. Sensors 2003, 3, 248-262.

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