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Detection of Antioxidative Activity of Plant Extracts at the DNA-Modified Screen-Printed Electrode
Department of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, 81237 Bratislava, Slovakia
Food Research Institute Bratislava, Biocentre Modra, 90001 Modra, Slovakia
UFZ Centre for Environmental Research Leipzig-Halle, D-04318 Leipzig, Germany
* Author to whom correspondence should be addressed.
Received: 12 October 2001; in revised form: / Accepted: 26 October 2001 / Published: 15 January 2002
Abstract: A simple procedure for the voltammetric detection of antioxidative activity of plant extracts based on the protection from DNA damage at the electrode surface is reported. A disposable electrochemical DNA biosensor fabricated as a carbon-based screen-printed electrode modified by a surface layer of the calf thymus double stranded (ds) DNA was used as a working electrode in combination with a silver/silver chloride reference electrode and a separate platinum auxiliary electrode. The [Co(phen)3]3+ ion served as the dsDNA redox marker and the [Fe(EDTA)]- complex with hydrogen peroxide under the electrochemical reduction of the iron atom were used as the DNA cleavage mixture. A remarkable antioxidative activity of phenolic antioxidants such as rosmarinic and caffeic acids as standards and the extracts of lemon balm, oregano, thyme and agrimony was found which is quite in agreement with an antiradical activity determined spectrophotometrically using 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical.
Keywords: DNA biosensor; Screen-printed electrode; Damage to DNA; Antioxidants; Plant extracts
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MDPI and ACS Style
Labuda, J.; Bučková, M.; Heilerová, L.; Čaniová-Žiaková, A.; Brandšteterová, E.; Mattusch, J.; Wennrich, R. Detection of Antioxidative Activity of Plant Extracts at the DNA-Modified Screen-Printed Electrode. Sensors 2002, 2, 1-10.
Labuda J, Bučková M, Heilerová L, Čaniová-Žiaková A, Brandšteterová E, Mattusch J, Wennrich R. Detection of Antioxidative Activity of Plant Extracts at the DNA-Modified Screen-Printed Electrode. Sensors. 2002; 2(1):1-10.
Labuda, J.; Bučková, M.; Heilerová, L.; Čaniová-Žiaková, A.; Brandšteterová, E.; Mattusch, J.; Wennrich, R. 2002. "Detection of Antioxidative Activity of Plant Extracts at the DNA-Modified Screen-Printed Electrode." Sensors 2, no. 1: 1-10.