Next Article in Journal
A (Bio-)Chemical Field-Effect Sensor with Macroporous Si as Substrate Material and a SiO2 / LPCVD-Si3N4 Double Layer as pH Transducer
Previous Article in Journal
A Disposable Sensor For Assessing Artocarpus heterophyllus L. (Jackfruit) Maturity
Sensors 2002, 2(1), 1-10; doi:10.3390/s20100001
Article

Detection of Antioxidative Activity of Plant Extracts at the DNA-Modified Screen-Printed Electrode

1,* , 1, 2, 1, 1, 3 and 3
1 Department of Analytical Chemistry, Faculty of Chemical and Food Technology, Slovak University of Technology, 81237 Bratislava, Slovakia 2 Food Research Institute Bratislava, Biocentre Modra, 90001 Modra, Slovakia 3 UFZ Centre for Environmental Research Leipzig-Halle, D-04318 Leipzig, Germany
* Author to whom correspondence should be addressed.
Received: 12 October 2001 / Accepted: 26 October 2001 / Published: 15 January 2002
Download PDF [140 KB, uploaded 20 June 2014]

Abstract

A simple procedure for the voltammetric detection of antioxidative activity of plant extracts based on the protection from DNA damage at the electrode surface is reported. A disposable electrochemical DNA biosensor fabricated as a carbon-based screen-printed electrode modified by a surface layer of the calf thymus double stranded (ds) DNA was used as a working electrode in combination with a silver/silver chloride reference electrode and a separate platinum auxiliary electrode. The [Co(phen)3]3+ ion served as the dsDNA redox marker and the [Fe(EDTA)]- complex with hydrogen peroxide under the electrochemical reduction of the iron atom were used as the DNA cleavage mixture. A remarkable antioxidative activity of phenolic antioxidants such as rosmarinic and caffeic acids as standards and the extracts of lemon balm, oregano, thyme and agrimony was found which is quite in agreement with an antiradical activity determined spectrophotometrically using 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical.
Keywords: DNA biosensor; Screen-printed electrode; Damage to DNA; Antioxidants; Plant extracts DNA biosensor; Screen-printed electrode; Damage to DNA; Antioxidants; Plant extracts
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Labuda, J.; Bučková, M.; Heilerová, L.; Čaniová-Žiaková, A.; Brandšteterová, E.; Mattusch, J.; Wennrich, R. Detection of Antioxidative Activity of Plant Extracts at the DNA-Modified Screen-Printed Electrode. Sensors 2002, 2, 1-10.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Sensors EISSN 1424-8220 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert