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Sensors 2018, 18(7), 2277; https://doi.org/10.3390/s18072277

A Sensitive Potentiometric Sensor for Isothermal Amplification-Coupled Detection of Nucleic Acids

1
Daegu Research Center for Medical Devices, Korea Institute of Machinery and Materials, Daegu 42994, Korea
2
Nano-Mechanical Systems, Korea Institute of Machinery and Materials, Daejeon 34103, Korea
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 1 June 2018 / Revised: 11 July 2018 / Accepted: 12 July 2018 / Published: 14 July 2018
(This article belongs to the Special Issue Label-Free Biosensors)
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Abstract

A disposable potentiometric sensor was newly developed for the amplification-coupled detection of nucleic acids. The hydrogen-ion is generally released during isothermal amplification of nucleic acids. The surface potential on the oxide-functionalized electrode of the extended gate was directly measured using full electrical circuits with the commercial metal-oxide semiconductor field-effect transistors (MOSFETs) and ring oscillator components, which resulted in cost-effective, portable and scalable real-time nucleic acid analysis. The current-starved ring oscillator changes surface potential to its frequency depending on the square of the variation in pH with a high signal-to-noise ratio during isothermal amplification. The device achieves a conversion rate of 20.5 kHz/mV and a detection resolution of 200 µV for the surface potential. It is demonstrated that the sensor successfully monitors in real-time isothermal amplification of the extracted nucleic acids from Salmonella pathogenic bacteria. The in situ variations in the frequency of the pH-sensitive sensor were compared with the results of both a conventional optical device and pH-meter during isothermal amplification. View Full-Text
Keywords: potentiometric sensor; surface potential; pH-sensitive sensor; full electrical readout; isothermal amplification; pathogenic bacteria; label-free potentiometric sensor; surface potential; pH-sensitive sensor; full electrical readout; isothermal amplification; pathogenic bacteria; label-free
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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    Description: Figure S1: Electropherograms of LAMP in the commercial or developed buffer, Figure S2: Temperature profiles after setting temperature at 65 oC
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Lee, K.-H.; Lee, D.; Yoon, J.; Kwon, O.; Lee, J. A Sensitive Potentiometric Sensor for Isothermal Amplification-Coupled Detection of Nucleic Acids. Sensors 2018, 18, 2277.

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