Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection
AbstractThe cytokine interleukin-13 (IL-13) plays a major role in airway inflammation and is a target of new anti-asthmatic drugs. Hence, IL-13 determination could be interesting in assessing therapy success. Thus, in this work an electrochemical immunosensor for IL-13 was developed and integrated into a fluidic system with temperature control for read-out. Therefore, two sets of results are presented. First, the sensor was set up in sandwich format on single-walled carbon nanotube electrodes and was read out by applying the hydrogen peroxide–hydroquinone–horseradish peroxidase (HRP) system. Second, a fluidic system was built up with an integrated heating function realized by Peltier elements that allowed a temperature-controlled read-out of the immunosensor in order to study the influence of temperature on the amperometric read-out. The sensor was characterized at the temperature optimum of HRP at 30 °C and at 12 °C as a reference for lower performance. These results were compared to a measurement without temperature control. At the optimum operation temperature of 30 °C, the highest sensitivity (slope) was obtained compared to lower temperatures and a limit of detection of 5.4 ng/mL of IL-13 was calculated. Taken together, this approach is a first step towards an automated electrochemical immunosensor platform and shows the potential of a temperature-controlled read-out. View Full-Text
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Metzner, J.; Luckert, K.; Lemuth, K.; Hämmerle, M.; Moos, R. Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection. Sensors 2018, 18, 1309.
Metzner J, Luckert K, Lemuth K, Hämmerle M, Moos R. Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection. Sensors. 2018; 18(5):1309.Chicago/Turabian Style
Metzner, Julia; Luckert, Katrin; Lemuth, Karin; Hämmerle, Martin; Moos, Ralf. 2018. "Towards an Electrochemical Immunosensor System with Temperature Control for Cytokine Detection." Sensors 18, no. 5: 1309.
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