Surface Plasmon Resonance and Bending Loss-Based U-Shaped Plastic Optical Fiber Biosensors
AbstractEscherichia coli (E. coli) is a large and diverse bacteria group that inhabits the intestinal tract of many mammals. Most E. coli strains are harmless, however some of them are pathogenic, meaning they can make one sick if ingested. By being in the feces of animals and humans, its presence in water and food is used as indicator of fecal contamination. The main method for this microorganism detection is the bacterial culture medium that is time-consuming and requires a laboratory with specialized personnel. Other sophisticated methods are still not fast enough because they require sending samples to a laboratory and with a high cost of analysis. In this paper, a gold-coated U-shaped plastic optical fiber (POF) biosensor for E. coli bacteria detection is presented. The biosensor works by intensity modulation principle excited by monochromatic light where the power absorption is imposed by predominant effect of either bending loss or surface plasmon resonance (SPR), depending on the gold thickness. Bacterial selectivity is obtained by antibody immobilization on the fiber surface. The biosensor showed a detection limit of 1.5 × 103 colony-forming units (CFU)/mL, demonstrating that the technology can be a portable, fast response and low-cost alternative to conventional methodologies for quality analysis of water and food. View Full-Text
Share & Cite This Article
Arcas, A.S.; Dutra, F.S.; Allil, R.C.S.B.; Werneck, M.M. Surface Plasmon Resonance and Bending Loss-Based U-Shaped Plastic Optical Fiber Biosensors. Sensors 2018, 18, 648.
Arcas AS, Dutra FS, Allil RCSB, Werneck MM. Surface Plasmon Resonance and Bending Loss-Based U-Shaped Plastic Optical Fiber Biosensors. Sensors. 2018; 18(2):648.Chicago/Turabian Style
Arcas, Ariadny S.; Dutra, Fábio S.; Allil, Regina C.S.B.; Werneck, Marcelo M. 2018. "Surface Plasmon Resonance and Bending Loss-Based U-Shaped Plastic Optical Fiber Biosensors." Sensors 18, no. 2: 648.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.