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Sensors 2018, 18(2), 648; https://doi.org/10.3390/s18020648

Surface Plasmon Resonance and Bending Loss-Based U-Shaped Plastic Optical Fiber Biosensors

1
Nanotechnology Engineering Program, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-901, Brazil
2
Petrobras’ Research and Development Center, CENPES, Rio de Janeiro 21941-915, Brazil
3
Electrical Engineering Program, COPPE, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-901, Brazil
*
Author to whom correspondence should be addressed.
Received: 22 November 2017 / Revised: 30 January 2018 / Accepted: 8 February 2018 / Published: 22 February 2018
(This article belongs to the Special Issue Optical Biochemical Sensor Systems and Applications)
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Abstract

Escherichia coli (E. coli) is a large and diverse bacteria group that inhabits the intestinal tract of many mammals. Most E. coli strains are harmless, however some of them are pathogenic, meaning they can make one sick if ingested. By being in the feces of animals and humans, its presence in water and food is used as indicator of fecal contamination. The main method for this microorganism detection is the bacterial culture medium that is time-consuming and requires a laboratory with specialized personnel. Other sophisticated methods are still not fast enough because they require sending samples to a laboratory and with a high cost of analysis. In this paper, a gold-coated U-shaped plastic optical fiber (POF) biosensor for E. coli bacteria detection is presented. The biosensor works by intensity modulation principle excited by monochromatic light where the power absorption is imposed by predominant effect of either bending loss or surface plasmon resonance (SPR), depending on the gold thickness. Bacterial selectivity is obtained by antibody immobilization on the fiber surface. The biosensor showed a detection limit of 1.5 × 103 colony-forming units (CFU)/mL, demonstrating that the technology can be a portable, fast response and low-cost alternative to conventional methodologies for quality analysis of water and food. View Full-Text
Keywords: biosensor; bacteria detection; bending loss; U-shaped fiber sensor; Escherichia coli; plastic optical fiber; refractive index; surface plasmon resonance biosensor; bacteria detection; bending loss; U-shaped fiber sensor; Escherichia coli; plastic optical fiber; refractive index; surface plasmon resonance
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Arcas, A.S.; Dutra, F.S.; Allil, R.C.S.B.; Werneck, M.M. Surface Plasmon Resonance and Bending Loss-Based U-Shaped Plastic Optical Fiber Biosensors. Sensors 2018, 18, 648.

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